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[1]项蕾蕾,李丹,孙雪丽,等.LED补光对香蕉组培苗增殖和生理生化指标的影响[J].应用与环境生物学报,2020,26(03):590-596.[doi:10.19675/j.cnki.1006-687x.2019.08038]
 XIANG Leilei,LI Dan,SUN Xueli,et al.Effects of LED supplementation on proliferation, and physiological and biochemical indices of tissue-cultured banana plantlets[J].Chinese Journal of Applied & Environmental Biology,2020,26(03):590-596.[doi:10.19675/j.cnki.1006-687x.2019.08038]
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LED补光对香蕉组培苗增殖和生理生化指标的影响
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
26卷
期数:
2020年03期
页码:
590-596
栏目:
研究论文
出版日期:
2020-06-25

文章信息/Info

Title:
Effects of LED supplementation on proliferation, and physiological and biochemical indices of tissue-cultured banana plantlets
作者:
项蕾蕾李丹孙雪丽田娜刘范付帅陈裕坤林玉玲程春振王天池?赖钟雄
福建农林大学园艺学院,园艺植物生物工程研究所 福州 350002
Author(s):
XIANG Leilei LI Dan SUN Xueli TIAN Na LIU Fan FU Shuai CHEN Yukun LIN Yuling CHENG Chunzhen WANG Tianchi? & LAI Zhongxiong?
College of Horticulture/Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University, Fuzhou 350002, China
关键词:
香蕉LED补光增殖系数生理生化指标叶绿素含量叶绿素荧光
Keywords:
banana LED supplementation proliferation coefficient physiology and biochemistry index chlorophyll content chlorophyll fluorescence
DOI:
10.19675/j.cnki.1006-687x.2019.08038
摘要:
为研究补充不同光质的LED光源对香蕉组培苗增殖和相关生理生化指标的影响,以‘天宝蕉’为材料,通过设置普通荧光 + LED红光(R)、普通荧光 + LED蓝光(B)、普通荧光 + LED绿光(G)、普通荧光+LED白光(W)、普通荧光 + LED暖白光(WW)、普通荧光 + LED暖黄光(WY),对照组普通荧光7种处理,比较不同LED补光对香蕉组培苗的增殖率、相关生理指标、叶绿素含量及叶绿素荧光参数的影响. 结果显示,WY处理下香蕉组培苗的增殖系数最大,B处理下香蕉苗的增殖系数最小;R处理下,植株高度最大,B处理下,植株假茎最粗,WW处理下,有最大的根长,W处理下根数、根粗、叶数、叶宽、叶长的数值最大,同时鲜重和含水量最高,壮苗系数最优. 与CK相比,R、B和G处理会降低叶绿素以及类胡萝卜素含量,而补充LED白光可以提高叶绿素以及类胡萝卜素含量. 此外,LED补光,尤其是R、W和G补光会引起香蕉组培苗叶片Fv/Fo、Fv/Fm和NPQ参数的显著增加和Y(Ⅱ)、ETR、和qP等参数的降低. 本研究表明在增殖培养阶段补充LED暖黄光,香蕉组培苗的增殖率最高;在生根培养阶段补充LED白光,香蕉组培苗的叶绿素含量、chla/chlb比值、类胡萝卜素含量最大,同时光合效率和壮苗系数最高;结果可为香蕉组培苗增殖和生根阶段的光质选择奠定基础. (图5 表4 参34)
Abstract:
This study aimed to investigate the effects of LED supplementation on the proliferation, and physiological and biochemical indexes of tissue-cultured banana plantlets. ‘Tianbaojiao’ bananas were subjected to seven different LED treatments: ordinary fluorescence plus LED red light (R), ordinary fluorescence plus LED blue light (B), ordinary fluorescence plus LED green light (G), ordinary fluorescence plus LED white light (W), ordinary fluorescence plus LED warm white light (WW), ordinary fluorescence plus LED warm yellow light (WY), and ordinary fluorescence (CK). Treatment effects on growth rate, physiological indexes, chlorophyll content, and chlorophyll fluorescence of banana plantlets were then compared. The multiplication coefficient of banana plantlets was found to be the highest under WY treatment and the smallest under B treatment. Plantlets were tallest under R treatment, the largest pseudostem diameter was recorded under B treatment, and the root length was greatest under WW treatment. The maximum root length, root number, root diameter, leaf number, leaf width, and leaf length were greatest under W treatment. Moreover, CK, R, B, and G treatments resulted in inhibited chlorophyll and carotenoid accumulation, while white light supplementation increased chlorophyll and carotenoid content. Plants in the W group also showed the highest fresh weight, water content, and optimum seedling coefficient. Furthermore, R and G treatments with monochromatic light decreased chlorophyll and carotenoid content, while white LED supplementation increased chlorophyll and carotenoid content. In addition, supplemental LED light, especially red, white, and green light significantly increases the parameters of Fv/Fo, Fv/Fm, and NPQ, but inhibits the Y(Ⅱ), ETR, and qP parameters. During multiplication culture processes, warm yellow light supplementation resulted in the highest plantlet growth rate. During rooting, white light LED supplementation is helpful for chlorophyll and carotenoid accumulation and provides the highest chla/chlb ratio, photosynthetic efficiency, and seedling-strengthening coefficient. Our study provides a basis for future LED light supplementation in banana tissue culture.

参考文献/References:

1 Wang YC, Zhang HX, Zhao B, Yun XF. Improved growth of Artemisia annua L. hairy roots and artemisinin production under red light conditions [J]. Biotechnol Let, 2001, 23 (23): 1971-1973
2 李青竹, 蔡友铭, 杨贞, 杨柳燕, 郑玉红, 孙翊, 李心, 许俊旭, 张永春. 不同LED光质对石蒜幼苗生长、生理和生物碱积累的影响[J]. 应用与环境生物学报, 2019, 25 (6): 1-10 [Li QZ, Cai YM, Yang Z, Yang LY, Zheng YH, Sun Y, Li X, Xu JX, Zhang YC. Effects of LED light quality on growth, physiology characteristics and alkaloids accumulations of Lycoris radiata [J]. Chin J Appl Environ Biol, 2019, 25 (6): 1-10]
3 Escobar MA, Franklin KA, Svenson AS, Salter MG. Light regulation of the Arabidopsis respiratory chain. Multiple discrete photoreceptor responses contribute to induction of type II NAD (P) H dehydrogenase genes [J]. Plant Physiol, 2004, 136 (1): 2710-2721
4 Li Q, Kubota C. Effects of supplemental light quality on growth and phytochemicals of baby leaf lettuce [J]. Environ Exper Bot, 2009, 67 (1): 59-64
5 Fan XX, Zang J, Xu ZG, Guo SR, Jiao XL. Effects of different light quality on growth, chlorophyll concentration and chlorophyll biosynthesis precursors of non-heading Chinese cabbage [J]. Acta Physiol Plant, 2013, 35 (9): 2721-2726
6 Dierck R, Dhooghe E, Van HJ, Dominique VDS, De KE. Light quality regulates plant architecture in different genotypes of, chrysanthemum morifolium, ramat [J]. Sci Hortic, 2017, 218: 177-186
7 Vogel IN, Macedo AF. Influence of IAA, TDZ, and light quality on asymbiotic germination, protocorm formation, and plantlet development of Cyrtopodium glutiniferum Raddi., a medicinal orchid [J]. Plant Cell Tissue Organ Cult, 2011, 104 (2): 147-155
8 Cope KR, Bugbee B. Spectral effects of light-emitting diodes on plant growth and development, the importance of green and blue light [C]. Hortic Sci, 2013, 48 (4): 504–509
9 Son KH, Park JH, Kim D, Oh MM. Leaf shape index, growth, and phytochemicals in two leaf lettuce cultivars grown under monochromatic light-emitting diodes [J]. Korean J Hortic Sci Technol, 2012, 30 (6): 664-672
10 Son KH, Oh MM. Growth, photosynthetic and antioxidant parameters of two lettuce cultivars as affected by red, green, and blue light-emitting diodes [J]. Hortic Environ Biotechnol, 2015, 56 (5): 639-653
11 Kim EY, Park SA, Park BJ, Lee Y, Oh MM. Growth and antioxidant phenolic compounds in cherry tomato seedlings grown under monochromatic light-emitting diodes [J]. Hortic Environ Biotechnol, 2015, 55 (6): 506-513
12 Zheng L, Van LMC. Chrysanthemum morphology, photosynthetic efficiency and antioxidant capacity are differentially modified by light quality [J]. J Plant Physiol, 2017, 213: 66-74
13 Wang H, Gu M, Cui J, Shi K, Zhou YH, Yu JQ. Effects of light quality on CO2 assimilation, chlorophyll-fluorescence quenching, expression of Calvin cycle genes and carbohydrate accumulation in Cucumis sativus [J]. J Photochem Photobiol B Biol, 2009, 96 (1): 30-37
14 Nhut DT, Hong LTA, Watanabe H, Goi M, Tanaka M. Growth of banana plantlets cultured in vitro under red and blue light-emitting diode (LED) irradiation source [J]. Acta Hortic, 2002 (575): 117-124
15 石颖. LED光源在天宝高蕉试管苗工厂化育苗上的应用[D]. 福州: 福建农林大学, 2015 [Shi Y. Application of LED light source in industrial seedling raising of plantain plantlets in vitro in Tianbao [D]. Fuzhou: Fujian Agricultural and Forestry University, 2015]
16 梁学芬, 蚁伟南, 颜梓兴, 肖安裕, 黄剑波. 不同光质的辅助光对香蕉组培苗的影响[J]. 中国南方果树, 2001, 30 (4): 34 [Liang XF, Yi WN, Yan ZX, Xiao AY, Huang JB. Effect of the supplement by different illumination on the growth of banana plantlets in vitro [J]. South Chin Fruits, 2001, 30 (4): 34]
17 余义强. 固体、液体培养和LED光源对粉蕉工厂化试管育苗的影响[D]. 福州: 福建农林大学, 2015 [Yu YQ. Effects of solid, liquid culture and LED light source on plantain seedling in vitro [D]. Fuzhou: Fujian Agricultural and Forestry University, 2015]
18 周成波, 张旭, 崔青青, 李曼, 张文东, 艾希珍, 毕焕改, 刘彬彬, 李清明. LED补光光质对小白菜生长及光合作用的影响[J]. 植物生理学报. 2017, 53 (6): 1030-1038 [Zhou CB, Zhang X, Cui QQ, Li M, Zhang WD, Ai XZ, Bi HG, Liu BB, Li QM. Effects of LED light supplementation on growth and photosynthesis of pakchoi [J]. Plant PhysiolJ, 2017, 53 (6): 1030- 1038]
19 刘建平, 王芳, 杜彩娴, 韩秀香, 张珂恒, 何建齐, 王悦萍. 香蕉新品种东蕉1号的组织培养技术研究[J]. 现代农业科技, 2018 (4): 68-69 [Liu JP, Wang F, Du CX, Han XX, Zhang KH, He JQ, Wang YP. Study on tissue culture techniques of new banana variety Dongjiao 1 [J]. Mod Agric Sci Technol, 2018 (4): 68-69]
20 薛婷婷, 韩梅琳, 孙晓红, 王继华. 菌糠西瓜、甜瓜育苗试验[J]. 江苏农业科学. 2015, 43 (4): 191-192 [Xue TT, Han ML, Sun XH, Wang JH. Experiment on seedling raising of fungi watermelon and melon [J]. Jiangsu Agric Sci, 2015, 43 (4): 191-192]
21 Yuan GF, Sun B, Yuan J, Wang QM. Effect of 1-methylcyclopropene on shelf life, visual quality, antioxidant enzymes and health-promoting compounds in broccoli florets [J]. Food Chemistry, 2010, 118 (3): 774-781
22 张真, 李胜, 李唯, 刘媛, 吴兵, 张青松, 李婷. 不同光质光对葡萄愈伤组织增殖和白藜芦醇含量的影响[J]. 植物生理学报, 2008, 44 (1): 106-108 [Zhang Z, LI Sheng, Li W, Liu Y, Wu B, Zhang QS, Li Ting. Effects of different light quality on callus proliferation and resveratrol content in grape [J]. Plant Physiol J, 2008, 44 (1): 106-108]
23 周吉源, 赵洁, 程井辰. 油菜花序轴组织培养中不同光质对形态发生的效应[J]. 植物学报, 1992 (s1): 24-24 [Zhou JY, Zhao J, Cheng JC. Effects of different light quality on morphology in cauliflower inflorescence axis tissue culture [J]. Plant J, 1992 (S1): 24]
24 王丽伟, 李岩, 辛国凤, 魏珉, 米庆华, 杨其长. 不同比例红蓝光对番茄幼苗生长和光合作用的影响[J]. 应用生态学报, 2017, 28 (5): 1595-1602 [Wang LW, Li Y, Xin GF, Wei M, Mi QH, Yang QC. Effects of different proportions of red and blue light on the growth and photosynthesis of tomato seedlings [J]. Chin J Appl Ecol, 2017, 28 (5): 1595-1602]
25 Yu WW, Liu Y, Song LL, Jacobs DF, Du XH, Ying YQ, Shao QS, Wu JS. Effect of differential light quality on morphology, photosynthesis, and antioxidant enzyme activity in Camptotheca acuminata seedlings [J]. J Plant Growth Reg, 2016, 36: 1-13
26 周成波, 张旭, 刘彬彬, 艾希珍, 毕焕改, 李清明. 补光光质对叶用莴苣光合特性的影响[J]. 植物生理学报, 2015, 51 (12): 2255-2262 [Zhou CB, Zhang X, Liu BB, Ai XZ, Bi HG, Li QM. Effects of light supplementation and light quality on photosynthetic characteristics of leaf lettuce [J]. Plant Physiol J, 2015, 51 (12): 2255-2262]
27 杜洪涛, 刘世琦, 蒲高斌,. 光质对彩色甜椒幼苗生长及叶绿素荧光特性的影响[J]. 西北农业学报, 2005, 14 (1): 41-45 [Du HT, Liu SQ, Pu GB. Effects of light qualities on growth and chlorophyll fluorescence parameters in leaves of color pepper seedling [J]. Acta Agric Bor-occid Sin, 2005, 14 (1): 41-45]
28 梁浩虹, 牟英辉, 方临志, 黄启良, 温志鹏, 卢文艺, 李蕊, 马稚昱. LED光质对台湾金线莲生长及品质的影响[J]. 现代农业装备, 2018, 236 (5): 28-33+39 [Liang HH, Mou YH, Fang LZ, Huang QL, Wen ZP, Lu WY, Li R, Ma ZY. Influence of LED on the Growth and Quality of Taiwan [J]. Mod Agric Equip, 2018, 236 (5): 28-33+39]
29 Yorio NC, Goins GD, Kagie HR, Wheeler RM, Sager JC. Improving spinach, radish, and lettuce growth under red light-emitting diodes (leds) with blue light supplementation [J]. Hortic Sci, 2001, 36 (2), 380-383.
30 刘晓英, 徐志刚, 常涛涛, 郭世荣. 不同光质LED弱光对樱桃番茄植株形态和光合性能的影响[J]. 西北植物学报, 2010, 30 (4): 725-732 [Liu XY, Xu ZG, Chang TT, Guo SR. Effects of different light quality LED weak light on plant morphology and photosynthetic performance of cherry tomato [J]. Acta Bot Bor-Occid Sin, 2010, 30 (4): 725-732]
31 徐凯, 郭延平, 张上隆. 不同光质对草莓叶片光合作用和叶绿素荧光的影响[J]. 中国农业科学, 2005, 38 (2): 369-375 [Xu K, Guo YP, Zhang SL. Effect of light quality on photosynthesis and chlorophyll fluorescence in strawberry leaves [J]. Sci Agric Sin, 2005, 38 (2): 369-375]
32 Zheng L, Van LMC. Effects of different irradiation levels of light quality on chrysanthemum [J]. Sci Hortic, 2018, 233: 124-131
33 赵占娟, 李光, 王秀生, 唐志远. 光质对绿豆幼苗叶片超微弱发光及叶绿素含量的影响[J]. 西北植物学报. 2009, 29 (7): 1465-1469 [Zhao ZJ, Li G, Wang XS, Tang ZY. Ultraweak biophoton emission and chlorophyll contents in leaves of mung bean seedlings under different spectrum lights [J]. Acta Bot Bor-Occid Sin, 2009, 29 (7): 1465-1469]
34 陈韵, 朱国胜, 郭巧生, 王长林, 刘作易. 光质对半夏生长及其药材品质的影响[J]. 中国中药杂志, 2013, 38 (24): 4257-4262 [Chen Y, Zhu GS, Guo QS, Wang CL, Liu ZY. Effect of light quality on the growth and quality of Pinellia ternata [J]. Chin J Chin Mat Med, 2013, 38 (24): 4257-4262]

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更新日期/Last Update: 2020-06-25