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[1]葛娜,杨玲,陈军文.不同浓度赤霉素和脱落酸对顽拗性三七种子后熟种胚发育和内源激素的影响[J].应用与环境生物学报,2020,26(03):574-581.[doi:10.19675/j.cnki.1006-687x.2019.08033]
 GE Na,,et al.Effects of varying gibberellin and abscisic acid levels on embryo development and endogenous hormones in recalcitrant Panax notoginseng seeds during the after-ripening process[J].Chinese Journal of Applied & Environmental Biology,2020,26(03):574-581.[doi:10.19675/j.cnki.1006-687x.2019.08033]
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不同浓度赤霉素和脱落酸对顽拗性三七种子后熟种胚发育和内源激素的影响
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
26卷
期数:
2020年03期
页码:
574-581
栏目:
研究论文
出版日期:
2020-06-25

文章信息/Info

Title:
Effects of varying gibberellin and abscisic acid levels on embryo development and endogenous hormones in recalcitrant Panax notoginseng seeds during the after-ripening process
作者:
葛娜杨玲陈军文
1云南农业大学农学与生物技术学院 昆明 650201 2云南农业大学云南省药用植物生物学重点实验室 昆明 650201 3云南农业大学西南中药材种质创新与利用国家地方联合工程研究中心 昆明 650201
Author(s):
GE Na1 2 3 YANG Ling1 2 3 & CHEN Junwen1 2 3?
1 College of Agriculture and Biotechnology, Yunnan Agricultural University, Kunming 650201, China 2 Yunnan Provincial Key Laboratory of Medicinal Plant Biology, Yunnan Agricultural University, Kunming 650201, China 3 National & Local Joint Engineering Research Center on Germplasms Utilization & Innovation of Chinese Medicinal Materials in Southwest, Yunnan Agricultural University, Kunming 650201, China
关键词:
三七顽拗性种子植物激素后熟种胚发育
Keywords:
Panax notoginseng recalcitrant seed phytohormone after-ripening embryo development
DOI:
10.19675/j.cnki.1006-687x.2019.08033
摘要:
为研究外源激素赤霉素(GA3)和脱落酸(ABA)对顽拗性三七种子后熟休眠和萌发的作用机理,用不同浓度的GA3和ABA处理三七种子,观察处理后种子后熟过程的种胚发育,并测定6种与调控种子发育相关的内源激素含量. 结果显示:三七种胚和胚乳长度的百分比(即胚率)随着后熟期延长逐渐增加,外源激素GA3 500 mg/L的处理显著提高种子的萌发率;ABA 10 mg/L的浓度处理则明显抑制种子的萌发. 经外源激素ABA和GA3处理后,三七种子内源激素ABA、GA3含量在后熟初始期显著高于对照组,分别为2.83 ng/g 和27.85 ng/g,随着后熟时间的延长逐渐降低;吲哚乙酸(IAA)含量也随后熟时间的推移逐渐降低,但与对照组无显著差异;水杨酸(SA)在三七后熟过程中含量基本保持不变;茉莉酸甲酯(JA)在对照组和ABA处理组中表现为逐渐升高的趋势,并在后熟50 d时达到最大值(1.25 ng/g),而在GA3处理组中表现为先升高后降低的趋势;反式玉米素(tZT)含量在对照组中基本保持不变,在GA3处理组中随后熟的延长逐渐升高,而在ABA处理组中则逐渐降低. 上述结果表明,外源激素处理可有效调控三七种子内源激素,从而间接调控三七种子的后熟休眠与萌发;其中,高浓度的GA3处理可以促进种子萌发、缩短后熟期,低浓度的ABA处理则显著延长顽拗性三七种子休眠期. (图6 表1 参43)
Abstract:
To understand the mechanisms behind the dormancy and germination of recalcitrant seed from Panax notoginseng (Burk.) F. H. Chen, seeds were treated by exogenous hormone gibberellin (GA3) and abscisic acid (ABA). Embryo development was then observed and six endogenous hormone levels were analyzed during the after-ripening process. The results showed that the ratio of embryos to endosperm increases gradually during the after-ripening process. Germination rate significantly increased when seeds were treated with exogenous hormone GA3 at a concentration of 500 mg/L, and it was significantly inhibited with treatment by ABA at a concentration of 10 mg/L. The concentration of endogenous hormones ABA and GA3 reached 2.83 ng/g and 27.85 ng/g, respectively. These concentrations were significantly higher in the treated seeds than in the control groups at the initial after-ripening stage and the two hormones gradually decreased during the after-ripening process. The IAA concentration also gradually decreased during the after-ripening process, but there was no significant difference between the two groups. The concentration of salicylic acid (SA) varied little during the process. Methyl jasmonate (JA) gradually increased in both the control groups and the ABA treated groups, with the maximum value reaching 1.25 ng/g in the ABA treatment group at 50 d of the after-ripening process. However, in the GA3 treatment group, JA initially increased and then decreased. The concentration of trans-zeicin (tZT) was generally unchanged in the control group, but gradually increased in the GA3 treatment group and gradually decreased in the ABA treatment group. Our results indicate that the application of exogenous phytohormones may effectively regulate endogenous phytohormones, thereby indirectly influencing the after-ripening process and germination of P. notoginseng seeds. In addition, high GA3 concentration may break dormancy and shorten the after-ripening process, while low ABA concentration may significantly prolong the dormancy of P. notoginseng seeds.

参考文献/References:

1 Roberts EH. Predicting the storage life of seeds [J]. Seed Sci Technol, 1973, 1: 499-514
2 Umarani R, Adhavan EK, Faisal MM. Understanding poor storage potential of recalcitrant seeds [J]. Curr Sci, 2015, 108: 2023-2034
3 Pammenter NW, Berjak, P. Physiology of desiccation-sensitive (recalcitrant) seeds and the implications for cryopreservation [J]. Int J Plant Sci, 2014, 175 (1): 21-28
4 Pritchard HW, Daws MI, Fletcher BJ, Gamane CS, Msanga HP, Omondi W. Ecological correlates of seed desiccation tolerance in tropical African dryland trees [J]. Amer J Bot, 2004, 6: 863-870
5 Graeber K, Nakabayashi K, Miatton E, Leubner-metzger G, Soppe WJJ. Molecular mechanisms of seed dormancy [J]. Plant Cell Environ, 2012, 35 (10): 1769-1786
6 Liu Y, Qiu YP, Zhang L. Dormancy breaking and storage behavior of Garcinia cowa Roxb. (Guttiferae) seeds: implication for ecological function and gemplasm conservation [J]. J Integra Plant Biol, 2005, 47: 38-49
7 彭业芳, 傅家瑞. 荔枝种子成熟后的萌发与超微结构观察研究[J]. 科学通报, 1995, 40 (5): 450-452 [Peng YF, Fu JR. Study on germination and ultrastructure of Litchi chinensis seeds after ripening [J]. Chin Sci Bull, 1995, 40 (5): 450-452]
8 宗梅, 蔡永平, 范志强. 板栗种子发育期间ABA等生理指标与脱水耐性的相关性研究[J]. 广西植物, 2011, 31 (6): 818-822 [Zong M, Cai YP, Fang ZQ. Study on correlation between physiological indexes as ABA, etc and desiccation tolerance in developing chestnut seeds [J]. Guihaia, 2011, 31 (6): 818-822]
9 Gasparin E, Faria JMR, JOS? AC, Hilhorst HWM. Physiological and ultrastructural responses during drying of recalcitrant seeds of Araucaria angustifolia [J]. Seed Sci Technol, 2019, 45 (1): 112-129
10 Yan A, Chen Z. The pivotal role of abscisic acid signaling during transition from seed maturation to germination [J]. Plant Cell Rep, 2016, 36 (5): 698-703
11 Shuai H, Meng Y, Luo X, Chen F, Zhou W, Dai Y, Qi Y, Du J, Yang F, Liu J, Yang W, Shu K. Exogenous auxin represses soybean seed germination through decreasing the gibberellin/abscisic acid (GA/ABA) ratio [J]. Sci Rep, 2017, 7 (1): 12620
12 Toh S, Kamiya Y, Kawakami N, Nambara E, Mccourt P, Tsuchiya Y. Thermo inhibition uncovers a role for strigolactones in Arabidopsis seed germination [J]. Plant Cell Physiol, 2012, 53 (1): 107-117
13 田美华, 赵正武, 唐安军. 苏铁种子的脱水敏感性及温湿条件对种子后熟的影响[J]. 植物生理学报, 2016, 52 (2): 225-233 [Tian MH, Zhao ZW, Tang AJ. Effects of dehydration sensitivity and temperature and humidity conditions of Cycas revoluta seeds on seed ripening [J]. Acta Phytophysiol Sin, 2016, 52 (2): 225-233]
14 苏立遥, 黄倏祺, 蒋梦琦, 厉雪, 徐小萍, 陈旭, 赖钟雄, 林玉玲. 龙眼MiR403及其候选靶标对外源激素的响应模式以及在龙眼体胚中的表达模式 [J]. 应用与环境生物学报, 2019, 25 (4): 977-984 [Su LY, Huang HQ, Jiang MQ, Li X, Chen X, Lai ZX, Lin YL. The response patterns of miR403 and its candidate targets to exogenous hormones and their expression profiles in longan somatic embryo [J]. Chin J Appl Environ Biol, 2019, 25 (4): 977-984]
15 张旺凡. 不同植物生长调节剂打破多花黄精种子休眠试验[J]. 中医药学报,2008, 36 (6): 42-43 [Zhang WF. Different plant growth regulators to break Palygunaum cyrtonema seed dormancy test [J]. J Chin Med Pharmacol, 2008, 36 (6): 42-43]
16 李磊, 孙雪婷, 张广辉, 龙光强, 孟珍贵, 杨生超. 脱水速率对顽拗性三七种子脱水敏感性和抗氧化酶活性的影响[J]. 种子, 2014, 33 (12): 1-5 [Li L, Sun XT, Zhang GH, Long GQ, Meng ZG, Yang SC. Effect of drying rates on the desiccation sensitivity and antioxidant enzyme activities of recalcitrant Panax notoginseng seeds [J]. Seed, 2014, 33 (12): 1-5]
17 崔秀明, 张燕. 三七种子生物学特性研究[J]. 中药材, 1993, 16 (12): 3-4 [Cui XM, Zhang Y. Study on biological characteristics of Panax notoginseng seeds [J]. J Chin Med Mater, 1993, 16 (12): 3-4]
18 崔秀明, 王朝梁, 李伟, 陈永明. 植物激素及生调节剂对三七种子的效应[J]. 中药材, 1994, 17 (2): 3-5 [Cui XM, Wang CL, Li W, Chen YM. Effects of phytohormones and growth regulators on Panax notoginseng seeds [J]. J Chin Med Mater, 1994, 17 (2): 3-5]
19 段承俐, 杨莉, 萧凤回. 三七种胚形态发育的解剖观察[J]. 种子, 2010, 29 (1): 1-7 [Duan CL, Yang L, Xiao FH. Anatomical observation on morphological development of Panax notoginseng embryo [J]. Seed, 2010, 29 (1): 1-7]
20 段承俐, 李章田, 丁金玲, 萧凤回. 三七种子的后熟生理特性研究[J]. 中国中药杂志, 2010, 35 (20): 2652-2656 [Duan CL, Li ZT, Ding JL, Xiao FH. Physiologic characteristics of Panax notoginseng seeds during after-ripening process [J]. China J Chin Mater Med, 2010, 35 (20): 2652-2656]
21 安娜, 崔秀明, 黄璐琦, 段承俐, 萧凤回. 三七种子后熟期的生理生化动态研究 Ⅱ. 代谢物质含量变化分析[J]. 西南农业学报, 2010, 23 (4): 1090-1093 [An N, Cui XM, Huang LQ, Duan CL, Xiao FH. Studies on variation of physiology and biochemistry during post-maturation of seeds in Panax notoginseng II. Analysis of variation of metabolic products in seeds. SW China J Agric Sci, 2010, 23 (4): 1090-1093]
22 付长珍, 郭宝林. 药用植物种子形态生理休眠研究方法概述[J]. 中国现代中药, 2013, 15 (10): 856-859 [Fu CL, Guo BL. Review on morphophysiological dormancy research method of medicinal plant seeds [J]. Mod Chin Med, 2013, 15 (10): 856-859]
23 杨凯, 李磊, 龙光强, 孟珍贵, 李龙根, 陈军文. 顽拗性三七种子后熟过程超微结构和抗氧化酶变化[J]. 广西植物, 2016, 36 (12): 1519-1525 [Yang K, Li L, Long GQ, Meng ZG, Li LG, Chen JW. Changes of antioxidant enzyme and ultrastructure in recalcitrant seeds of Panax notoginseng during after-ripening process [J]. Guihaia, 2016, 36 (12): 1519-1525]
24 李磊, 孙雪婷, 张广辉, 龙光强, 孟珍贵, 杨生超, 陈军文. 脱水速率对顽拗性三七种子脱水敏感性和抗氧化酶活性的影响 [J]. 种子, 2014, 33 (12): 1-5 [Li L, Sun XT, Zhang GH, Long GQ, Meng ZG, Yang SC, Chen JW. Effect of drying rates on the desiccation sensitivity and antioxidant enzyme activities of recalcitrant Panax notoginseng seeds [J]. Seed, 2014, 33 (12): 1-5]
25 杨凯, 杨景煌, 刘绍伟, 孟珍贵, 谢世清, 陈军文. 三七种子后熟过程种胚发育和6种内源激素的动态变化[J]. 中药材, 2018, 41 (3): 519-523 [Yang K, Yang JH, Liu SW, Meng ZG, Xie SQ, Chen JW. Embryo development and dynamic changes of six endogenous phytohormones during after-ripening process of Panax notoginseng seeds [J]. J Chin Med Mater, 2018, 41 (3): 519-523]
26 Yang K, Yang L, Fan W, Long GQ, Xie SQ, Meng ZG, Zhang GH, Yang SC, Chen JW. Illumina-based transcriptomic analysis on recalcitrant seeds of Panax notoginseng for the dormancy release during the after-ripening process [J]. Physiol Plant, 2019, 167: 597-612
27 Pan X, Welti R, Wang X. Quantitative analysis of major plant hormones in crude plant extracts by high-performance liquid chromatography-mass spectrometry [J]. Nat Prot, 2010, 5 (6): 986-992
28 Graeber K, Linkies A, Müller K, Wunchova A, Rott A, Leubner-Metzger G. Cross-species approaches to seed dormancy and germination: conservation and biodiversity of ABA-regulated mechanisms and the Brassicaceae DOG1 genes [J]. Plant Mol Biol, 2010, 73 (1-2): 67-87
29 Gazzarrini S, Tsai AYL. Hormone cross-talk during seed germination [J]. Essays Biochem, 2015, 58: 151-164
30 Zhou L, Wu J, Wang S. Low-temperature stratification strategies and growth regulators for rapid induction of Paris polyphylla var. yunnanensis seed germination [J]. Plant Growth Regul, 2003, 41 (2): 179-183
31 Yamaguchi S, Kamiya Y. Gibberellins and light-stimulated seed germination [J]. J Plant Growth Regul, 2001, 20 (4): 369-376
32 安娜. 三七果实发育和种子后熟特性研究[D]. 昆明: 云南农业大学, 2006 [An N. Studies on fruit development and seed after-ripening characteristics of Panax notoginseng [D]. Kunming: Yunnan Agricultural University, 2006]
33 伍静辉, 谢楚萍, 田长恩, 周玉萍. 脱落酸调控种子休眠和萌发的分子机制 [J]. 植物学报, 2018, 53 (4): 542-555 [Wu JH, Xie CP, Tian CE, Zhou YP. Molecular mechanism of abscisic acid regulation during seed dormancy and germination. Chin Bull Bot, 2018, 53 (4): 542-555]
34 郑郁善. ABA对米槠和格氏栲种子发育的影响[J]. 应用与环境生物学报, 1999, 5 (5): 444-449 [Zheng YS. Effect of ABA on seed development of Castanopsis Carlesii and C. kawakamii [J]. Chin J Appl Environ Biol, 1999, 5 (5): 444-449]
35 Paw?owski TA, Staszak AM. Analysis of the embryo proteome of sycamore (Acer pseudoplatanus L.) seeds reveals a distinct class of proteins regulating dormancy release. J Plant Physiol, 2016, 195: 9-22
36 Li L, Zhao Y, McCaig BC, Wingerd BA, Wang J, Whalon ME, Pichersky E, Howea GA. The tomato homolog of CORONATINE-INSENSITIVE1 is required for the maternal control of seed maturation, jasmonate-signaled defense responses, and glandular trichome development [J]. Plant Cell, 2004, 16 (1): 126-143
37 朱霞, 胡勇, 王晓丽, 陈诗. 水杨酸浸种对三七种子萌发的影响[J]. 甘肃农业大学学报, 2013, 48 (2): 55-57 [Zhu X, Hu Y, Wang XL, Chen S. Effects of seed soaking with SA on germination of Panax notoginseng seeds. J Gansu Agric Univ, 2013, 48 (2): 55-57]
38 宋松泉, 刘军, 徐恒恒, 张琪, 黄荟, 伍贤进. 乙烯的生物合成与信号及其对种子萌发和休眠的调控[J]. 作物学报, 2019, 45 (7): 969-981 [Song SQ, Liu J, Zhang Q, Huang H, Wu XJ. Biosynthesis and signaling of ethylene and their regulation on seed germination and dormancy [J]. Acta Agron Sin, 2019, 45 (7): 969-981]
39 陈明权, 黄海波, 蒲立立. 猴耳环种子萌发过程中内源激素动态变化的研究[J]. 种子, 2014, 33 (3): 66-68 [Chen MQ, Huang HB, Pu LL. Dynamic changes of endogenous phytohormones during germination process of Pithecellobium clypearia seeds [J]. Seed, 2014, 33 (3): 66-68]
40 Wang JG, Gao XM, Ma ZL, Chen J, Liu YN, Shi WQ. Metabolomic and transcriptomic profiling of three types of litchi pericarps reveals that changes in the hormone balance constitute the molecular basis of the fruit cracking susceptibility of Litchi chinensis cv. Baitangying [J]. Mol Biol Rep, 2019, 46 (5): 5295-5308
41 Bicalho EM, Pintó-Marijuan M, Morales M, Müller M, Munné-Bosch S, Garcia QS. Control of macaw palm seed germination by the gibberellin/abscisic acid balance [J]. Plant Biol, 2015, 17 (5): 990-995
42 赵永华, 杨世林, 刘惠卿, 刘铁城. 西洋参种胚形态后熟过程中种子内源激素变化动态及其对种胚发育的调节[J]. 中草药, 2001, 32 (2): 159-162 [Zhao YH, Yang SL, Liu HQ, Liu TC. Dynamics of endogenous hormone in American ginseng seed during morphological after-ripening period and their regulation to embryo development [J]. China Tradit Herb Drugs, 2001, 32 (2): 159-162]
43 司倩倩, 臧德奎, 刘丹, 褚泽龙. 玉铃花种子休眠原因及其内源激素含量变化[J]. 北方园艺, 2017 (6): 91-95 [Si QQ, Zang DK, Chu ZL. Causes of dormancy and change of endogenous hormone content in Styrax obassia seeds [J]. North Hort, 2017 (6): 91-95]

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更新日期/Last Update: 2020-06-25