|本期目录/Table of Contents|

[1]周应敏,詹明晔,张姝,等.亚成体大熊猫肠道微生物结构的季节差异及其与肠道纤维素酶活性的相关性[J].应用与环境生物学报,2020,26(03):499-505.[doi:10.19675/j.cnki.1006-687x.2019.07027]
 ZHOU Yingmin,ZHAN Mingye,ZHANG Shu,et al.Difference in intestinal microbial structure of sub-adult giant pandas in different seasons and its correlation with cellulase activity[J].Chinese Journal of Applied & Environmental Biology,2020,26(03):499-505.[doi:10.19675/j.cnki.1006-687x.2019.07027]
点击复制

亚成体大熊猫肠道微生物结构的季节差异及其与肠道纤维素酶活性的相关性
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
26卷
期数:
2020年03期
页码:
499-505
栏目:
研究论文
出版日期:
2020-06-25

文章信息/Info

Title:
Difference in intestinal microbial structure of sub-adult giant pandas in different seasons and its correlation with cellulase activity
作者:
周应敏詹明晔张姝王磊黄炎刘晓强王一张志忠
1中国大熊猫保护研究中心大熊猫国家公园珍稀动物保护生物学国家林业和草原局重点实验室 都江堰 611830 2同济大学环境科学与工程学院,上海市污染控制与生态安全研究院 上海 200092 3上海动物园 上海 200335 4沈阳动物园管理有限公司 沈阳 110000
Author(s):
ZHOU Yingmin1 ZHAN Mingye2 ZHANG Shu3 WANG Lei2 HUANG Yan1 LIU Xiaoqiang1 WANG Yi4 & ZHANG Zhizhong1?
1 Key Laboratory of State Forestry and Grassland Administration on Conservation Biology of Rare Animals in the Giant Panda National Park, China Conservation and Research Center for the Giant Panda, Dujiangyan 611830, China 2 College of Environmental Science and Engineering in Tongji University, Institute of Pollution Control and Ecological Safety in Shanghai, Shanghai 200092, China 3 Shanghai Zoo, Shanghai 200335, China 4 Shenyang Zoo Management Co. Ltd., Shengyang 110000, China
关键词:
亚成体大熊猫季节肠道微生物结构纤维素酶活性Pearson相关性分析
Keywords:
sub-adult giant panda season gut microbial structure cellulose activity Pearson correlation analysis
DOI:
10.19675/j.cnki.1006-687x.2019.07027
摘要:
大熊猫以低营养高纤维的竹子作为主要食物来源,但其消化系统结构保留了肉食动物的特点,不具备消化纤维素的能力,因此肠道菌群对其消化纤维素具有重要意义. 为了解不同季节亚成体大熊猫肠道微生物结构的变化及其与肠道纤维素消化能力的内在联系,通过高通量测序技术分析4只亚成体大熊猫肠道细菌和真菌多样性的季节差异,并检测不同季节的肠道纤维素酶活性. 结果发现不同季节亚成体大熊猫肠道细菌、真菌结构和丰富度存在较大差异. 复杂的肠道微生物组成,特别是优势细菌结构主要决定了亚成体大熊猫消化利用纤维素的能力. 冬季(2月)肠道微生物结构较复杂,微生物丰富度也相对较高,细菌的优势度高于其他季节. 秋季(11月)和冬季(2月),亚成体大熊猫的纤维素酶活性较高,冬季纤维素酶活性约为其他季节的5倍. 亚成体大熊猫的纤维素酶活与其肠道优势细菌Streptococcus丰度的季节变化具有相似的趋势,相关性系数为0.582,是大熊猫分解利用纤维素的重要贡献者. 因此,亚成体大熊猫不同季节的肠道微生物结构演变对其纤维素消化具有重要影响. (图3 表6 参28)
Abstract:
Giant pandas consume low nutrition and high fiber bamboo as their main food, but their digestive system structure retains the characteristics of carnivores and does not possess the ability to digest cellulose. Therefore, the gut microorganisms of giant pandas are important for the digestion of cellulose. In this study, we aimed to investigate the changes in intestinal microbial structure of sub-adult giant pandas in different seasons and their relationship with the ability to digest cellulose. We used high-throughput sequencing technology to analyze the bacterial and fungal diversity of four sub-adult giant pandas in different seasons, and simultaneously their cellulase activity was assessed. We further investigated the correlation between the gut microbial structure of these sub-adult giant pandas in different seasons and their cellulase activities. The results showed that there were significant differences in the intestinal bacterial and fungal structure and richness of sub-adult giant pandas in different seasons. The structures of complex intestinal microbes, especially the predominant bacteria, are more conducive to the ability of sub-adult giant panda to digest cellulose. The microbial structure in winter (February) was complicated and the microbial richness was relatively high. The dominant degree of bacteria in winter was significantly higher than those in other seasons. In autumn (November) and winter (February), the cellulase activity of sub-adult giant pandas was generally high, and in winter it was about 5 times higher than those in other seasons. It was closely related to the changes in the abundance of intestinal dominant bacterial strain, Streptococcus, for which the correlation coefficient is 0.582. In general, the evolution of intestinal microbial structure of sub-adult giant pandas in different seasons has an important impact on their ability to digest cellulose.

参考文献/References:

1 詹明晔, 付小花, 张姝, 张鑫, 杨海迪, 俞锦华, 王爱善, 王磊. 不同地区成体大熊猫肠道微生物结构差异性及其与纤维素消化能力的相关性[J]. 应用与环境生物学报, 2019, 25 (3): 736-742 [Zhan MY, Fu XH, Zhang S, Zhang X, Yang HD, Yu JH, Wang AS, Wang L. Differences of the intestinal microbial structure of adult giant panda in different regions and its correlation with the digestibility of cellulose [J]. Chin J Appl Environ Biol, 2019, 25 (3): 736-742]
2 何东阳. 大熊猫取食竹选择、消化率及营养和能量对策的研究[D]. 北京: 北京林业大学, 2010 [He DY. Study on bamboo selection, digestibility, nutrition and energy countermeasures for giant pandas [D]. Beijing: Beijing Forestry University, 2010]
3 Zhang W, Liu W, Hou R, Zhang L, Schmitz ES, Sun HB, Xie JJ, Zhang YF, Wang CD, Li LF, Yue BS, Huang H, Wang HR, Shen FJ, Zhang ZH. Age-associated microbiome shows the giant panda lives on hemicelluloses, not on cellulose [J]. ISME J, 2018, 12: 1319-1328
4 Guo W, Mishra S, Zhao J. Metagenomic study suggests that the gut microbiota of the giant panda (Ailuropoda melanoleuca) may not be specialized for fiber fermentation [J]. Front Microbiol, 2018, 9: doi 10.3389/fmicb.2018.00229
5 张文平, 张志和. 大熊猫肠道宏基因组发育分析[R].成都: 第十三届全国野生动物生态与资源保护学术研讨会暨第六届中国西部动物学学术研讨会, 2017 [Zhang WP, Zhang ZH. Macro genome development analysis of giant panda’s intestine [R]. Chengdu: The 13th National Symposium on Wildlife Conservation and the 6th Western China Zoological Symposium, 2017]
6 Zhu L, Wu Q, Dai JY, Zhang BS, Wei FW. Evidence of cellulose metabolism by the giant panda gut microbiome [J]. PNAS, 2011, 108 (43): 17714-17719
7 Wei F, Wang X, Wu Q . The giant panda gut microbiome [J]. Trends Microbiol, 2015, 23 (8): 450-452
8 Guo L. PGRP-SC2 promotes gut immune homeostasis to limit commensal dysbiosis and extend lifespan [J]. Cell, 2014, 156 (1-2): 109-122
9 Chu H. Gene-microbiota interactions contribute to the pathogenesis of inflammatory bowel disease [J]. Science, 2016, 352 (6289): 1116
10 Perry RJ. Acetate mediates a microbiome-brain-β cell axis promoting metabolic syndrome [J]. Nature, 2016, 534 (7606): 213-217
11 李蓓, 郭莉娟, 龙梅, 汤纯香, 周晓平, 罗燕, 邹立扣. 圈养大熊猫肠道微生物分离、 鉴定及细菌耐药性研究[J]. 四川动物, 2014, 33 (2): 161-166 [Li B, Guo LJ, Long M, Tang CX. Zhou XP, Luo Y, Zou LK. Isolation and antimicrobial resistance of bacteria from intestinal tract of giant panda [J]. Sichuan Zoo, 2014, 33 (2): 161-166]
12 曹涵文, 吴珑韬, 甘乾福, 鲍若虹, 梁学武. 熊猫粪便中纤维素降解菌的筛选与鉴定[J]. 家畜生态学报, 2015, 36 (6): 19-25 [CAO HW, Wu LT, Gan QF, Bao RH, Liang XW. Isolation and identification of cellulose-degradation bacteria from panda dung [J]. Acta Ecol Anim Dom, 2015, 36 (6): 19-25]
13 马缨, 殷红涛. 大熊猫源纤维素分解菌的分离及产酶条件研究[J]. 黑龙江畜牧兽医, 2017 (2): 172-174 [Ma Y, Yin HY. Isolation and enzyme production of giant panda derived cellulolytic bacteria [J]. Heilongjiang Anim Husb, 2017 (2): 172-174]
14 刘艳红, 钟志军, 艾生权, 王承东, 夏玉, 吴虹林, 李才武, 杨海迪, 林姗姗, 彭广能. 亚成体大熊猫肠道纤维素降解真菌的分离与鉴定[J]. 中国兽医科学, 2015 (1): 43-49 [Liu YH, Zhong ZJ, Ai SQ, Wang CD, Xia Y, Wu HL, Li CW, Yang HD, Lin SS, Peng GN. Isolation and identification of intestinal cellulose-degrading fungi in sub-adult giant pandas [J]. Chin Vet Sci, 2015 (1): 43-49]
15 李倜, 赵珂, 黄炎, 王梦婷, 李静, 张潇月, 李玥, 李德生. 不同年龄大熊猫肠道可培养芽孢杆菌的多样性及部分功能特性分析[J]. 微生物学通报, 2019, 46 (10): 2719-2729 [Li T, Zhao K, Huang Y, Wang MT, Li J, Zhang XY, Li Y, Li DS. Diversity and functional characteristics of bacillus isolated from gut of giant panda (Ailuropoda melanoleuca) at different age [J]. Microbiol China, 2019, 46 (10): 2719-2729]
16 刘海波, 绳秀珍, 唐小千, 邢婧, 战文斌. 培养条件对海豚链球菌的生长及其胞外产物蛋白组成的影响[J]. 中国动物检疫, 2016, 33 (5): 85-89 [Liu HB, Sheng XZ, Tang XQ, Xing J, Zhan WB. Effects of culture conditions on the growth of Streptococcus iniae and the protein composition of extracellular products [J]. Chin Anim Health Insp, 2016, 33 (5): 85-89]
17 Carey HV, Walters WA, Knight R. Seasonal restructuring of the ground squirrel gut microbiota over the annual hibernation cycle [J]. Am J Physiol Reg Integ Comp Physiol, 2013, 304 (1): R33-R42
18 王燚, 何廷美, 钟志军, 王承东, 何永果, 程安春, 李才武, 崔婷婷, 李德生, 彭广能. 不同季节亚成体大熊猫肠道菌群ERIC-PCR指纹图谱分析[J]. 中国兽医科学, 2011, 41 (8): 778-783 [Wang Y, He TM, Zhong ZJ, Wang CD, He YG, Cheng AC, Li CW, Cui TT, Li DS, Peng GN. ERIC-PCR Fingerprint analysis of intestinal microflora of sub-adult giant pandas in different seasons [J]. Chin Vet Sci, 2011, 41 (8): 778-783]
19 Xue Z, Zhang W, Wang L. The bamboo-eating giant panda harbors a carnivore-like gut microbiota, with excessive seasonal variations [J]. mBio, 2015, 6 (3): 2-15
20 徐昶, 龙敏南, 邬小兵, 徐惠娟, 陈重安, 张凤章, 许良树. 高产纤维素酶菌株的筛选及产酶条件研究[J]. 厦门大学学报(自然科学版), 2005, 44 (1): 107-111 [Xu C, Long MN, Wu XB, Xu HJ, Chen ZA, Zhang FZ, Xu LS. Screening and characterization of a high cellulase producing strain Aspergillus glaucus XC9 [J]. J Xiamen Univ (Nat Sci), 2005, 44 (1): 107-111]
21 袁楠, 亢宗静, 卢圣鄂, 王莹燕, 张小平, 辜运富. 富集培养下的若尔盖高原湿地低温纤维素降解细菌群落结构[J]. 应用与环境生物学报, 2016, 22 (3): 402-408 [Yuan N, Kang ZJ, Lu SE, Wang YY, Zhang XP, Gu YF. Community structures of the cold-adapted cellulose-degrading bacteria in the Zoige plateau wetland under enrichment culture conditions [J]. Chin J Appl Environ Biol, 2016, 22 (3): 402-408]
22 曾文慧, 钟俊鸿, 李秋健, 刘炳荣. 反应温度对台湾乳白蚁内外源纤维素酶活力的差异影响[J]. 江苏农业科学, 2017, 45 (8): 91-94 [Zeng WH, ZhongJH, Li QJ, Liu BR. Effects of reaction temperature on endogenous and exogenous cellulase activities of C. formosanus in Taiwan [J]. Jiangsu Agric Sci, 2017, 45 (8): 91-94]
23 Dutilleul P. The Mantel test versus Pearson’s correlation analysis: assessment of the differences for biological and environmental studies [J]. J Agric Biol Environ Stat, 2000, 5 (2): 131-150
24 Huang L, Gao X, Liu M. Correlation among soil microorganisms, soil enzyme activities, and removal rates of pollutants in three constructed wetlands purifying micro-polluted river water [J]. Ecol Eng, 2012, 46: 98-106
25 彭广能, 熊焰, 李德生, 张和民, 何廷美. 亚成体大熊猫肠道正常菌群变化的初步研究[J]. 四川畜牧兽医, 1999 (5): 23 [Peng GN, Xiong Y, Li DS, He TM. A Preliminary study on the changes of normal intestinal flora in sub-adult giant panda [J]. Sichuan Anim Husb Vet Med, 1999 (5): 23]
26 王燚. 基于ERIC-PCR和16S rDNA-RFLP技术对亚成体大熊猫肠道菌群结构的研究[D]. 雅安: 四川农业大学, 2011 [Wang W. Study on the intestinal flora of sub-adult giant pandas based on ERIC-PCR and 16S rDNA-RFLP technology [D]. Ya′an: Sichuan Agricultural University, 2011]
27 艾生权, 夏玉, 钟志军, 王承东, 刘艳红, 吴虹林, 李才武, 杨海迪, 林姗姗, 石锦江, 彭广能. 亚成体大熊猫肠道真菌的分离与鉴定[J]. 四川动物, 2014, 33 (4): 522-527 [Ai SQ, Xia Y, Zhong ZJ, Wang CD, Liu YH, Wu HL, Li CW, Yang HD, Lin SS, Shi JJ, Peng GN. Isolation and identification of intestinal fungi from sub-adult giant panda [J]. Sichuan Anim, 2014, 33 (4): 522-527]
28 王卫云, 李大彪, 于永强, 邢媛媛, 李子健. 瘤胃4株纤维降解细菌的分离鉴定及其纤维降解特性[J]. 畜牧兽医学报, 2016, 47 (11): 2294-2300 [Wang WY, Li DB, Yu YQ, Xin YY, Li ZJ. Isolation and identification of four fiber degrading bacteria from rumen and their fiber degradation characteristics [J]. J Anim Husb Vet Med, 2016, 47 (11): 2294-2300]

更新日期/Last Update: 2020-06-25