|本期目录/Table of Contents|

[1]田娜,刘范,孙雪丽,等.水培炼苗对香蕉组培移栽苗生长及光合特性的影响[J].应用与环境生物学报,2020,26(03):582-589.[doi:10.19675/j.cnki.1006-687x.2019.06029]
 TIAN Na,LIU Fan,SUN Xueli,et al.Effects of hydroponic culture hardening on the growth and photosynthetic characteristics of banana seedlings[J].Chinese Journal of Applied & Environmental Biology,2020,26(03):582-589.[doi:10.19675/j.cnki.1006-687x.2019.06029]
点击复制

水培炼苗对香蕉组培移栽苗生长及光合特性的影响
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
26卷
期数:
2020年03期
页码:
582-589
栏目:
研究论文
出版日期:
2020-06-25

文章信息/Info

Title:
Effects of hydroponic culture hardening on the growth and photosynthetic characteristics of banana seedlings
作者:
田娜刘范孙雪丽车婧如项蕾蕾赖钟雄程春振
福建农林大学园艺学院,园艺植物生物工程研究所 福州 350002
Author(s):
TIAN Na LIU Fan SUN Xueli CHE Jingru XIANG Leilei LAI Zhongxiong? & CHENG Chunzhen?
College of Horticulture/Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
关键词:
水培炼苗香蕉植株生长根系活力光合特性
Keywords:
hydroponic culture hardening banana plant growth root activity photosynthetic characteristics
DOI:
10.19675/j.cnki.1006-687x.2019.06029
摘要:
为了解采用改良Hoagland溶液进行水培炼苗对香蕉组培苗生长和光合特性的影响,以‘巴西蕉’组培苗为材料,进行直接移栽(G1)、正常炼苗移栽(G2)和水培一周后移栽(G3)处理,比较3组香蕉苗形态、生理特性和生长相关指标的差异. 结果显示,与G1和G2组相比,G3组香蕉幼苗根长、地上部和地下部干重、根冠比极显著增加(P < 0.01),根数显著增多(P < 0.05). 移栽后4个月,G3组株高、叶宽、叶长极显著大于G2组(P < 0.01),大于G1组,但差异不显著. 植株叶片叶绿素含量和根系活力由高到低均为G3组、G2组、G1组,且G3组极显著高于其他两组(P < 0.01). 移栽后4个月,G3组叶片叶绿素a、叶绿素b、总叶绿素和类胡萝卜素含量与G1组相比分别增加49.0%、50.1%、49.4%和58.5%,与G2组相比分别增加23.98%、28.38%、25.43%和26.77%;G3组根系活力分别约为G1和G2组的3.04倍和1.86倍;G3组植株净光合速率、蒸腾速率、气孔导度、实际光合效率(YII)、电子传递速率(ETR)、光化学猝灭系数(qP)也均极显著高于其他两组(P < 0.01). 本研究表明水培炼苗可以提高香蕉苗根系活力,加快植株生长,提高叶片叶绿素含量和光合效率,增强植株生物量的积累,说明水培炼苗具有应用于香蕉种苗繁育的巨大潜力. (图7 表2 参33)
Abstract:
The aim of this study is to investigate the effects of hydroponic culture hardening using modified Hoagland solution on the growth and photosynthetic characteristics of tissue cultured banana seedlings. Micro-propagated ‘Brazil’ banana seedlings were divided into three groups: direct transplanting (G1), transplanting after normal hardening (G2), and one week of hydroponic culture before transplanting (G3). Following transplantation, the morphological and physiological characteristics and growth related indexes of banana seedlings from these three groups were compared. Results showed that some growth and development indexes of G3 seedlings, including root length, aboveground and underground dry weight, and root-shoot ratio were significantly higher than those of G1 and G2 (P < 0.01). Furthermore, the root number of G3 seedlings was significantly greater than that of the other two groups (P < 0.05). Four months after transplanting, the plant height, leaf width, and leaf length of G3 plants were significantly higher than those of G2 (P < 0.01). They were also higher than those of G1, although not significantly. The leaf chlorophyll content and root activity indexes were highest in G3, followed by G2, then G1, with these two indexes being significantly higher in G3 than in the other two groups (P < 0.01). At four months post-transplantation, the chlorophyll a, chlorophyll b, total chlorophyll, and carotenoid contents in G3 leaves were 49.0%, 50.1%, 49.4%, and 58.5% higher, respectively, than G1 leaf contents. Similarly, they were 23.98%, 28.38%, 25.43%, and 26.77% higher, respectively, than contents of G2 leaves. The root activity of G3 plants was approximately 3.04 times and 1.86 times that of G1 and G2, respectively. Additionally, the net photosynthetic rate, transpiration rate, stomatal conductance, actual photosynthetic rate (YII), electron transport rate (ETR), and photochemical quenching coefficient (qP) of G3 seedlings were all found to be significantly higher than in the other two groups (P < 0.01). Our results show that hydroponic culture prior to transplantation can increase root activity, enhance plant growth, and increase leaf chlorophyll content, and significantly promote plant photosynthetic efficiency and biomass accumulation in banana seedlings. This indicates that hydroponic culture hardening has great potential to be used in banana seedling propagation.

参考文献/References:

1 Lee SJ, Lee JY. Beneficial bacteria and fungi in hydroponic systems: types and characteristics of hydroponic food production methods [J]. Sci Hortic, 2015, 195: 206-215
2 李心悦, 张道远, 李进. 一种快速获得野苹果组培苗的方法[J]. 北方园艺, 2018 (14): 31-37 [Li XY, Zhang DY, Li J. A quick method for acquiring Malus sieversii’s tissue culture seedlings [J]. North Hortic, 2018 (14): 31-37]
3 王娜, 窦恺, 王志英, 张荣沭, 王玉成, 刘志华. 山新杨组培苗生根移栽方法[J]. 植物研究, 2014, 34 (3): 380-385 [Wang N, Dou K, Wang ZY, Zhang RS, Wang YC, Liu ZH. Rooting and transplanting method of Populus davidiana × P. bolleana tissue culture seedlings [J]. Plant Res, 2014, 34 (3): 380-385]
4 胡凤, 杨万年. 大豆组培苗水培生根与培养基生根比较研究[J]. 大豆科学, 2013, 32 (3): 333-335 [Hu F, Yang WN. Tap water can be used as medium for root induction and growth during tissue culture of soybean [J]. Soybean Sci, 2013, 32 (3): 333-335]
5 Buah JN, Tachie-Menson JW. Suitability of bud manipulation technique as an alternative to tissue culture in the production of suckers for plantains and bananas [J]. Biotechnology, 2015, 14 (1): 41-46
6 Khoozani AA, Birch J, Bekhit AEDA. Production, application and health effects of banana pulp and peel flour in the food industry [J]. J Food Sci Technol, 2019, 56 (2): 548-559
7 Waman AA, Bohra P, Sathyanarayana BN, Gourish RK, Ashok TH. Micropropagules can profitably save the choicest silk banana from extinction [J]. PNAS, 2014, 84 (3): 847-854
8 Lee TJ, Zobayed SMA, Firmani F, Park EJ. A novel automated transplanting system for plant tissue culture [J]. Biosyst Eng, 2019, 181: 63-72
9 邝瑞彬, 魏岳荣, 邓贵明, 李春雨, 左存武, 胡春华, 易干军. 香蕉高效组培快繁技术的研究[J]. 果树学报, 2016, 33 (10): 1315-1320 [Kuang RB, Wei YR, Deng GM, Li CY, Zuo CW, Hu CH, Yi GJ. Efficient micropropagation technology for banana seedling production [J]. J Fruit Sci, 2016, 33 (10): 1315-1320]
10 刘建平, 王芳, 杜彩娴, 韩秀香, 张珂恒, 何建齐, 王悦萍. 香蕉新品种东蕉1号的组织培养技术研究[J]. 现代农业科技, 2018 (4): 68-69+72 [Liu JP, Wang F, Du CX, Han XX, Zhang KH, He JQ, Wang YP. Study on tissue culture technology of new banana variety Dongjiao No.1 [J]. Mod Agric Sci Technol, 2018 (4): 68-69+72]
11 李谦盛, 樊晓亮, 方俊, 邓敏. 牛耳朵组培苗驯化移栽基质配方试验[J]. 上海应用技术学院学报(自然科学版), 2016, 16 (2):184-188 [Li QS, Fan XL, Fang J, Deng M. Experiment on the formula of growth media for Chirita eburnean tissue culture plantlet transplant [J]. J Shanghai Inst Technol (Nat Sci Ed), 2016, 16 (2): 184-188]
12 韦绍龙, 杨柳, 韦莉萍, 韦弟, 李朝生, 李小泉, 黄素梅, 覃柳燕, 田丹丹. 不同组培方法对香蕉组培苗假植阶段生理特征的影响[J]. 西南农业学报, 2016, 29 (6): 1285-1290 [Wei SL, Yang L, Wei LP, Wei D, Li CS, Li XQ, Huang SM, Qin LY, Tian DD. Effect of different tissue culture methods on physiological characteristics of banana plantlets at transplanting stage [J]. Southwest Agric J, 2016, 29 (6): 1285-1290]
13 王甲水, 徐碧云, 金志强, 刘菊华,贾彩红, 张建斌. 香蕉苗的水培方法和系统: CN102845287A [P]. 20130102 [Wang JS, Xu BY, Jin ZQ, Liu JH, Jia CH, Zhang JB. A hydroponic culture method and system for banana: CN102845287A [P]. 20130102]
14 Badr-Elden, Nower A, Elezaby A. Minimal cost production of banana (Musa spp.) using modified MS medium in vitro and acclimatization in hydroponics system ex vitro [J]. Biosci Res, 2017, 14 (3): 548-557
15 黄俊生,刘磊,梁昌聪,杨腊英,黄小娟. 一种水培香蕉组培苗的方法: CN102613053A [P]. 20120801 [Huang JS, Liu L, Liang CC, Yang LM, Huang XJ. A banana hydroponic culture method: CN102613053A [P]. 20120801 ]
16 符少萍, 李瑞梅, 惠杜娟, 冀乙萌, 郭建春. 木薯试管苗炼苗移栽技术研究[J]. 广东农业科学, 2011, 38 (11): 39-40+47 [Fu SP, Li RM, Hui DJ, Ji YM, Guo JC. Study on transplanting techniques of tissue cultural shoots of cassava [J]. Guangdong Agric Sci, 2011, 38 (11): 39-40+47]
17 程春振, 马文昇, 刘炜婳, 张梓浩, 齐全, 孙雪丽, 张永艳, 赖钟雄. 三明野生蕉和天宝蕉对Foc TR4侵染早期应答的差异[J]. 福建农林大学学报 (自然科学版), 2017, 46 (4): 397-401 [Cheng CZ, Ma WS, Liu WH, Zhang ZH, Qi Q, Sun XL, Zhang YY, Lai ZX. Comparisons of early responses of ‘Tianbaojiao’ banana and Sanming wild banana to Foc TR4 infection [J]. J Fujian Agric For Univ (Nat Sci Ed), 2017, 46 (4): 397-401]
18 Zhang X, Huang G, Bian X, Zhao Q. Effects of root interaction and nitrogen fertilization on the chlorophyll content, root activity, photosynthetic characteristics of intercropped soybean and microbial quantity in the rhizosphere [J]. Plant Soil Environ, 2013, 59 (2): 80-88
19 邱念伟, 王修顺, 杨发斌, 杨晓刚, 杨文, 刁润洁, 王秀, 崔静, 周峰. 叶绿素的快速提取与精密测定[J]. 植物学报, 2016, 51 (5): 667-678 [Qiu NW, Wang XS, Yang FB, Yang XG, Yang W, Diao RJ, Wang X, Cui J, Zhou F. Fast extraction and precise determination of chlorophyll [J]. Plant J, 2016, 51 (5): 667-678]
20 Casta?eda-Méndez O, Ogawa S, Medina A, Chavarriaga P, Selvaraj MG. A simple hydroponic hardening system and the effect of nitrogen source on the acclimation of in vitro cassava (Manihot esculenta Crantz) [J]. In Vitro Cell Dev Biol Plant, 2017, 53 (2): 75-85
21 郑姗, 张小艳, 张立杰, 谢丽雪, 李韬. 水培对蓝莓幼苗生长及光合性能的影响[J]. 福建农业学报, 2019, 34 (3): 306-312 [Zheng S, Zhang XY, Zhang LJ, Xie LX, Li D. Effects of hydroponic cultivation on growth and photosynthesis of blueberry seedlings [J]. Fujian J Agric Sci, 2019, 34 (3): 306-312]
22 刘世全, 曹红霞, 张建青, 胡笑涛. 不同水氮供应对小南瓜根系生长、产量和水氮利用效率的影响[J]. 中国农业科学, 2014, 47 (7): 1362-1371 [Liu SQ, Cao HX, Zhang JQ, Hu XT. Effects of different water and nitrogen supplies on root growth, yield and water and nitrogen use efficiency of small pumpkin [J]. Chin Agric Sci, 2014, 47 (7): 1362-1371]
23 生利霞, 束怀瑞. 低氧胁迫对平邑甜茶根系活力及氮代谢相关酶活性的影响[J]. 园艺学报, 2008, 25 (1): 7-12 [Sheng LX, Shu HR. Effects of hypoxia stress on root activity, respiratory rate and the activities of enzymes involved in nitrogen metabolism in roots of Malus hupehensis Rehd. [J]. Acta Hortic Sin, 2008, 25 (1): 7-12]
24 张晓勇, 唐道彬, 王季春, 蒋玉春, 敬夫, 卢会翔, 罗玉龙, 黄廷荣, 舒进康. 水培营养液离子浓度对脱毒马铃薯生长发育和光合性能的影响[J]. 西南农业学报, 2017, 30 (2): 345-353 [Zhang XY, Tang DB, Wang JC, Jiang YC, Jing F, Lu HX, Luo YL, Huang YR, Shu JR, Effect of different ionic concentration on plant growth and photosynthetic characteristisin virus-free potato by hydroponic system [J]. Southwest Agric J, 2017, 30 (2): 345-353]
25 黄小娟, 杨腊英, 谢德啸, 周端咏, 魏巍, 郭利佳, 黄俊生. 巴西香蕉苗静置水培营养液配方的初步筛选[J]. 热带农业科学, 2012, 32 (9): 49-52 [Huang XJ, Yang LM, Xie DX, Zhou DY, Wei W, Guo LJ, Huang JS. Screening of the nutrient solution formulas for static hydroponics of banana (Musa AAA cavendish subgroup ‘Brazil’) plantlets [J]. Trop Agric Sci, 2012, 32 (9): 49-52]
26 王文琳, 周长芳, 周屿, 朱洪光, 沈文燕. 沼液对水培蕹菜生长和光合特性的影响[J]. 江苏农业科学, 2018, 46 (12): 114-117 [Wang WL, Zhou CF, Zhou Y, Zhu HG, Shen WY. Effects of biogas slurry on growth and photosynthesis of hydroponic Ipomoea aquatica Fork. [J]. Jiangsu Agric Sci, 2008, 46 (12): 114-117]
27 蔡文博, 段虹, 王军, 朱元娣. 4个鲜食葡萄品种组培快繁体系的建立[J]. 核农学报, 2019, 33 (2): 248-254 [Cai WB, Duan H, Wang J, Zhu YD. Establishment of in vitro rapid micropropagation of four table grape cultivars [J]. J Nucl Agric, 2019, 33 (2): 248-254]
28 李福慧, 毛娟, 陈佰鸿, 米宝琴, 谢永东. 甜樱桃试管苗移栽过程中根系活力、叶片光合特性及超微结构的变化[J]. 甘肃农业大学学报, 2016, 51 (1): 77-83 [Li FH, Mao J, Chen BH, Mi BQ, Xie YD. Changes in root vigor, photosynthetic properties and ultrastructures of Prunus aviumtube seedlings during transplantation [J]. J Gansu Agric Univ, 2016, 51 (1): 77-83]
29 洪森荣. 南城淮山脱毒苗移栽驯化期的光合生理[J]. 基因组学与应用生物学, 2017, 36 (11): 4823-4828 [Hong SR. Photosynthetic physiology of virus-free plantlets of Dioscorea opposite thunb. cv. Nancheng during transplanting and domestication [J]. Genomics Appl Biol, 2017, 36 (11): 4823-4828]
30 李丹, 黄玉吉, 朱宁, 刘范, 田娜, 徐涵, 程春振, 赖钟雄. 三明野生蕉抗寒特性的形态学与生理生化研究[J]. 果树学报, 2019, 36 (1): 78-87 [Li D, Huang YJ, Zhu N, Liu F, Tian N, Xu H, Cheng CZ, Lai ZX. A study on the phenotypical, physiological and biochemical characteristics of the cold resistant Sanming wild banana (Musa itinerans) [J]. J Fruit Sci, 2019, 36 (1): 78-87]
31 柏祥, 温瑀, 魏国印, 古小治. 湿地水深梯度对芦竹 (Arundo donax) 生长及叶绿素荧光特性的影响[J]. 应用与环境生物学报, 2015, 21 (6): 1157-1161 [Bai X, Wen Y, Wei GY, Gu XZ. Effects of water depth gradient on growth and chlorophyll fluorescence characteristics of Arundo donax [J]. Chin J Appl Environ Biol, 2015, 21 (6): 1157-1161]
32 李键, 刘奕, 吴承祯, 洪滔, 林勇明, 林晗, 陈灿, 洪伟. 木麻黄的两种化感物质对其水培幼苗叶绿素荧光参数的影响[J]. 应用与环境生物学报, 2013, 19 (5): 781-786 [Li J, Liu Y, Wu CZ, Hong T, Lin YM, Lin H, Chen C, Hong W. Effect of two allelochemicals of Casuarina equisetifolia L. on chlorophyll fluorescence parameters in hydroponically cultured seedling [J]. Chin J Appl Environ Biol, 2013, 19 (5): 781-786]
33 徐德聪, 吕芳德, 栗彬, 袁维风, 钱玉梅. 不同品种美国山核桃叶绿素荧光特性的比较[J]. 果树学报, 2008, 25 (5): 671-676 [Xu DC, Lv FD, Li B, Yuan WF, Qian YM. Comparison of chlorophyll fluorescence characteristics of different varieties of Pecan [J]. J Fruit Sci, 2008, 25 (5): 671-676]

相似文献/References:

[1]袁先福,孙玉菡,朱成之,等.轮作联用生物有机肥促进香蕉生长[J].应用与环境生物学报,2018,24(01):60.[doi:10.19675/j.cnki.1006-687x.2017.03022]
 YUAN Xianfu,SUN Yuhan,ZHU Chengzhi,et al.Rotation combined with bio-organic fertilizer application to promote banana growth[J].Chinese Journal of Applied & Environmental Biology,2018,24(03):60.[doi:10.19675/j.cnki.1006-687x.2017.03022]
[2]黄玉吉,赖钟雄.香蕉几丁质酶基因ChiI2超表达载体和干涉表达载体的构建[J].应用与环境生物学报,2019,25(03):672.[doi:10.19675/j.cnki.1006-687x.201809019]
 HUANG Yuji LAI Zhongxiong**.Over-expression and RNAi expression vector construction for the ChiI2 gene of banana[J].Chinese Journal of Applied & Environmental Biology,2019,25(03):672.[doi:10.19675/j.cnki.1006-687x.201809019]
[3]孙雪丽,刘范,田娜,等.香蕉MaMPK1基因的克隆与表达模式[J].应用与环境生物学报,2019,25(04):985.[doi:10.19675/j.cnki.1006-687x.2018.10027]
 SUN Xueli,LIU Fan,TIAN Na,et al.Cloning and expression pattern analysis of the banana MaMPK1 gene[J].Chinese Journal of Applied & Environmental Biology,2019,25(03):985.[doi:10.19675/j.cnki.1006-687x.2018.10027]
[4]项蕾蕾,李丹,孙雪丽,等.LED补光对香蕉组培苗增殖和生理生化指标的影响[J].应用与环境生物学报,2020,26(03):590.[doi:10.19675/j.cnki.1006-687x.2019.08038]
 XIANG Leilei,LI Dan,SUN Xueli,et al.Effects of LED supplementation on proliferation, and physiological and biochemical indices of tissue-cultured banana plantlets[J].Chinese Journal of Applied & Environmental Biology,2020,26(03):590.[doi:10.19675/j.cnki.1006-687x.2019.08038]
[5]刘彦英,倪珊珊,王晓,等.香蕉MaPFK基因家族鉴定、MaPFK3克隆和表达分析[J].应用与环境生物学报,2020,26(05):1.[doi:10.19675/j.cnki.1006-687x.2019.12042]
 LIU Yanying,NI Shanshan,WANG Xiao,et al.Identication and expression Analysis of MaPFK Gene Family, and MaPFK3 Cloning in Banana[J].Chinese Journal of Applied & Environmental Biology,2020,26(03):1.[doi:10.19675/j.cnki.1006-687x.2019.12042]
[6]刘嘉鹏# 刘萍元# 孙雪丽 刘范 田娜 伍俊为 程春振**.香蕉含VQ基序蛋白基因MaVQ1的克隆与表达[J].应用与环境生物学报,2021,27(02):1.[doi:10.19675/j.cnki.1006-687x.2020.03026]
 LIU Jiapeng#,LIU Pingyuan#,SUN Xueli,et al.Cloning and expression analysis of a VQ motif containing protein gene in banana[J].Chinese Journal of Applied & Environmental Biology,2021,27(03):1.[doi:10.19675/j.cnki.1006-687x.2020.03026]

更新日期/Last Update: 2020-06-25