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[1]张富美,侯瑞.一株槭射脉革菌MY51的分离鉴定及对染料的脱色能力[J].应用与环境生物学报,2020,26(02):332-338.[doi:10.19675/j.cnki.1006-687x.2019.05052]
 ZHANG Fumei & HOU Rui.Isolation and identification of strain Phlebia acerina MY51 and its decolorization to dyes[J].Chinese Journal of Applied & Environmental Biology,2020,26(02):332-338.[doi:10.19675/j.cnki.1006-687x.2019.05052]
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一株槭射脉革菌MY51的分离鉴定及对染料的脱色能力
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
26卷
期数:
2020年02期
页码:
332-338
栏目:
研究论文
出版日期:
2020-04-25

文章信息/Info

Title:
Isolation and identification of strain Phlebia acerina MY51 and its decolorization to dyes
作者:
张富美侯瑞
贵州大学林学院 贵阳 550025
Author(s):
ZHANG Fumei & HOU Rui?
College of Forestry, Guizhou University, Guiyang 550025, China
关键词:
内生真菌白腐真菌槭射脉革菌木质素降解酶染料脱色
Keywords:
endophytic fungi white rot fungi Phlebia acerina lignin-degrading enzyme decolorization
DOI:
10.19675/j.cnki.1006-687x.2019.05052
摘要:
通过对兔眼蓝莓叶片组织中分离得到的内生真菌MY51进行鉴定和产木质素降解酶活性检测,探讨该菌株对染料的脱色能力. 通过形态学和分子生物学分类法对菌株MY51进行鉴定;对菌株MY51所产3种木质素降解酶酶活曲线进行测定;利用菌株MY51对固体条件下8种染料进行脱色能力的检测,筛选出较易脱色的染料后,以脱色效果较好的染料模拟污水染料,研究菌株MY51在静态条件下对不同浓度固体染料的脱色能力. 结果表明,菌株MY51为白腐真菌——槭射脉革菌(Phlebia acerina),该菌株可以产木质素过氧化物酶、漆酶和锰过氧化物酶等木质素降解酶;且木质素过氧化物酶和锰过氧化物酶活性均在培养第8天达到最大值,分别为11.05、1.21 U/L;漆酶活性在第10天达到最大值18.52 U/L;菌株MY51对不同的染料均有脱色效果,对活性红和活性黑的脱色效果最显著;但染料浓度较高时会对菌株MY51的脱色产生一定抑制作用,脱色15 d后,MY51对质量浓度为10、50、100、250和500 mg/L活性红脱色率分别为98.2%、94.5%、87.8%、88.3%和85.6%;对质量浓度为10、50、100、250和500 mg/L的活性黑脱色率分别为98%、93%、83.3%、74.5%和65.5%. 本研究表明菌株MY51对活性染料具有较好脱色能力,在染料废水处理领域具有一定的应用前景. (图10 参31)
Abstract:
The endophytic fungus MY51 isolated from Vaccinium ashei Reade leaves was identified and the activity of its lignin-degrading enzyme was monitored to explore the decolorizing ability of the strain to dyes. MY51 was identified by morphological and molecular biological classification methods and the activity curves of three lignin-degrading enzymes produced by the MY51 strain were determined. The decolorization ability of MY51 to eight dyes was tested. The dye that was the easiest to be decolorized was screened out and used to simulate sewage dyes. The results showed that MY51 is a white rot fungus Phlebia acerina, which produces lignin-degrading enzymes such as lignin peroxidase, laccase, and manganese peroxidase. The lignin peroxidase and manganese peroxidase activities reached their maximum values, 11.05 U/L and 1.21 U/L, respectively, on the 8th day of culturing and the laccase activity reached its maximum value of 18.52 U/L on the 10th day. MY51 decolorized eight different dyes and had the most significant decolorization effect on reactive red and reactive black. However, higher dye concentration inhibited the decolorization ability of MY51. After 15 days of decolorization, the decolorization rates of 10 mg/L, 50 mg/L, 100 mg/L, 250 mg/L, and 500 mg/L reactive red were 98.2%, 94.5%, 87.8%, 88.3%, and 85.6%, respectively. The decolorization rates of reactive black with mass concentrations of 10 mg/L, 50 mg/L, 100 mg/L, 250 mg/L, and 500 mg/L were 98%, 93%, 83.3%, 74.5%, and 65.5%, respectively. Therefore, MY51 showed good decolorization ability toward reactive dyes and displayed a specific application prospect for dye wastewater treatment.

参考文献/References:

1 郭顺星. 药用植物内生真菌研究现状和发展趋势[J]. 菌物学报, 2018, 37 (1): 1-13 [Guo SX. The recent progress and prospects of research on endophytic fungi in medicinal plants [J]. Mycosystema, 2018, 37 (1): 1-13]
2 Schulz B, Boyle C, Draeger S, R?mmert AK, Krohn K. Endophytic fungi: a source of novel biologically active secondary metabolites [J]. Mycol Res, 2002, 106 (9): 996-1004
3 Lin X, Lu C, Huang Y, Zheng Z, Su W, Shen Y. Endophytic fungi from a pharmaceutical plant, Camptotheca acuminata: isolation, identification and bioactivity [J]. World J Microbiol Biotechnol, 2007, 23 (7): 1037-1040
4 Stierle A, Strobel GA, Stierle DB. Taxol and taxane production by Taxomyces andreanae, an endophytic fungus of pacific yew [J]. Science, 1993, 260 (5105): 214-216
5 Tan RX, Zou WX. Endophytes: a rich source of functional metabolites [J]. Nat Prod Rep, 2011, 18 (4): 448 -459
6 李永, 朴春根, 郭利民, 常聚普, 王海明, 贺伟, 谢守江, 郭民伟. 两种杨树树皮内生真菌多样性及优势种群动态变化[J]. 林业科学研究, 2013, 26 (3): 292-298 [Li Y, Pu CG, Guo LM, Chang JP, Wang HM, He W, Xie SJ, Guo MW. Predominant species dynamic and diversity of fungal endophytesin barks of two Populus cultivars [J]. For Res, 2013, 26 (3): 292-298]
7 袁继鑫, 侯智霞. 蓝莓菌根研究进展[J]. 中国果树, 2012 (4): 65-68+71 [Yuan JX, Hou ZX. Research progress of blueberry mycorrhizal fungi [J]. Chin Fruits, 2012 (4): 65-68+71]
8 刘静, 刘凤红, 宿红艳, 宋方圆, 程显好. 接种深色有隔内生真菌对蓝莓果实品质的影响[J]. 北方园艺, 2016 (20): 33-36 [Liu J, Liu FH, Su HY, Song FY, Cheng XH. Effect of inoculation DSE fungi on blueberry fruit quality [J]. North Hortic, 2016 (20): 33-36 ]
9 刘凤红. 蓝莓根系内生真菌多样性的研究[J]. 北方园艺, 2015 (21): 25-30 [Liu FH. Diversity of endophytic fungi in roots of blueberry [J]. Nor Hortic, 2015 (21): 25-30]
10 Li ZJ, Shen XY, Hou CL. Fungal endophytes of South China blueberry (Vaccinium dunalianum var. urophyllum) [J]. Let Appl Microbiol, 2016, 63 (6): 482-487
11 Céline L, Arthur QMM, Eliane L, Nathalie SD. How significant are endophytic fungi in bromeliad seeds and seedlings? effects on germination, survival and performance of two epiphytic plant species [J]. Fung Ecol, 2019, 39: 296-306
12 刘军, 刘艳明, 徐在超, 王卓娅, 黄雅丽,邓祖军. 檀香内生真菌多样性及其抗菌与促生特性的研究[J]. 中国中药杂志, 2018, 43 (17): 3477-3483 [Liu J, Liu YM, Xu C, Wang ZY, Huang YL, Deng ZJ. Diversity, antibacterial activites and growth promoting characteristics of endophytic fungi from sandal (Santalum album) [J]. Chin J Chin Mat Med, 2018, 43 (17): 3477-3483]
13 Waqas M, Khan AL, Kamran M, Hamayun M, Kang SM, Kim YH, Lee IJ. Endophytic fungi produce gibberellins and indoleacetic acid and promotes host-plant growth during stress [J]. Molecules, 2012, 17 (12): 10754-10773
14 Terhonen E, Susanna K, Sun H, Asiegbu FO. Endophytic fungi of Norway spruce roots in boreal pristine mire, drained peatland and mineral soil and their inhibitory effect on Heterobasidion parviporum in vitro [J]. Fung Ecol, 2014, 9 (1): 17-26
15 Li P, Wu Z, Liu T, Wang YA. Biodiversity, phylogeny, and antifungal functions of endophytic fungi associated with Zanthoxylum bungeanum [J]. Inter Jf Mol Sci, 2016, 17 (9): 1541
16 Passari A. Distribution and antimicrobial potential of endophytic fungi associated with ethnomedicinal plant Melastoma malabathricum L. [J]. J Environ Biol, 2016, 37 (2): 229
17 Navada KK, Sanjeev G, Kulal A. Enhanced biodegradation and kinetics of anthraquinone dye by laccase from an electron beam irradiated endophytic fungus [J]. Inter Biodeter Biodegr, 2018, 132: 241-250
18 Zhang ZC, Wang J, Fan YJ, Liu L, Shun QS, Shi WJ, Liu XC, Wang F. The synergistic effect of lignin peroxidase and cellulase in Aspergillus oryzae solid‐state fermentation substrate on enzyme‐catalyzed oxidative degradation of lignin [J]. J Chem Technol Biotechnol, 2019, 94 (5): 1480-1487
19 Heidary M, Khoobi M, Ghasemi S, Habibi Z, Faramarzi MA. Cheminform abstract: synthesis of quinazolinones from alcohols via laccase-mediated tandem oxidation [J]. Adv Synth Catal, 2014, 36: 1789-1794
20 Ngieng NS, Zulkharnain A, Roslan HA, Husaini A. Cell thermolysis—a simple and fast approach for isolation of bacterial laccases with potential to decolorize industrial dyes [J]. Process Biochem, 2017, 56: 171-176
22 刘忠川, 王刚刚.真菌漆酶结构与功能硏究进展[J]. 生物物理学报, 2013, 29 (9): 629-6445 [Liu ZC, Wang GG. Advances in structure and function of fungal Laccase [J]. Acta Biophy Sin, 2013, 29 (9): 629-6445]
23 Corrêa, Rúbia CG, Rhoden SA, Mota TR, Azevedo JLA, Pamphile JA, De Souza CGM. Endophytic fungi: expanding the arsenal of industrial enzyme producers [J]. J Ind Microbiol Biotechnol, 2014, 41 (10): 1467-1478
24 Sun J, Guo N, Niu LL, Wang QF, Zang YP, Zu YG, Fu YJ. Production of laccase by a new Myrothecium verrucaria MD-R-16 isolated from pigeon pea [Cajanus cajan (L.) Millsp.] and its application on dye decolorization [J]. Molecules, 2017, 22 (4): e673
25 Wesenberg D, Kyriakides I, Agathos SN. White-rot fungi and their enzymes for the treatment of industrial dye effluents [J]. Biotechnol Adv, 2003, 22 (1-2): 161-187
26 胡渤洋, 王寿南, 陈青君, 张国庆, 杨佳玥, 徐鑫, 韩鹏, 张宇轩, 李兵. 一种白腐真菌的分离、鉴定、培养及产漆酶条件[J]. 应用与环境生物学报, 2018, 24 (2): 367-373 [Hu BY, Wang SN, Chen QJ, Zhang GQ, Yang JY, Xu X, Han P, Zhang YX, Li B. isolation, identification, culture conditions, and laccase production of white rot fungus [J]. Chin J Appl Environ Biol, 2018, 24 (2): 367-373]
27 罗鑫, 覃育贤, 于存. 白囊耙齿菌产锰过氧化物酶条件优化及其对染料的脱色[J]. 菌物学报, 2018, 37 (9): 1233-1242 [Luo X, Qin YX, Yu C. Optimization of Irpex lacteus culture conditions for manganese peroxidase production and dye decolorization ability of the enzyme [J]. Mycosystema, 2018, 37 (9): 1233-1242]
28 Kumara R, Negia S, Sharmaa P, Prasher IB, Chaudhary S, Dhau JS, Umar A. Wastewater cleanup using Phlebia acerina fungi: an insight into mycoremediation [J]. J Environ Manage, 2018, 228: 130-139
29 贾晓, 朱小佩, 罗春芳, 叶佑丕, 卢东升. 板栗内生真菌B2菌株鉴定及其抑菌特征研究[J]. 信阳师范学院学报 (自然科学版),2011, 24 (2): 209-211 [Jia X, Zhu XP, Luo CF, Ye YP, Lu DS. Research of the B2 of endophytic fungi from Castanea millissima and its inhibition of fungi characteristics [J]. J Xinyang Nor Univ (Nat Sci Ed), 2011, 24 (2): 209-211]
30 袁海生, 戴玉成, 魏玉莲. 一株槭射脉革菌菌株及其在甲霜灵农药残留降解中的应用:中国专利, CN 103122316 A [P]. 2013-05-29 [Yuan HS, Dai YC, Wei YL. Application on degradation of strain Phlebia acerina in metalaxyl pesticide residues: China, CN 103122316 A [P]. 2013-05-29]
31 于存, 池玉杰.一色齿毛菌CB1对活性染料的脱色研究[J]. 菌物学报, 2017, 36 (6): 743-751 [Yu C, Chi YJ. Cerrena unicolor CB1 capability in reactive dye decolorization [J]. Mycosystema, 2017, 36 (6): 743-751]

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更新日期/Last Update: 2020-04-25