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[1]胡泳华,周媛,严重玲,等.铅胁迫对双孢蘑菇积累铅及抗氧化酶活性的影响[J].应用与环境生物学报,2020,26(02):312-318.[doi:10.19675/j.cnki.1006-687x.2019.05041]
 HU Yonghua,ZHOU Yuan,et al.Effect of lead stress on lead accumulation ability and antioxidant enzyme activity of the mushroom Agaricus bisporus[J].Chinese Journal of Applied & Environmental Biology,2020,26(02):312-318.[doi:10.19675/j.cnki.1006-687x.2019.05041]
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铅胁迫对双孢蘑菇积累铅及抗氧化酶活性的影响
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
26卷
期数:
2020年02期
页码:
312-318
栏目:
研究论文
出版日期:
2020-04-25

文章信息/Info

Title:
Effect of lead stress on lead accumulation ability and antioxidant enzyme activity of the mushroom Agaricus bisporus
作者:
胡泳华周媛严重玲刘俊敏张维友王勤
1闽南师范大学生物科学与技术学院 漳州 363000 2厦门大学生命科学学院,滨海湿地生态系统教育部重点实验室 厦门 361102
Author(s):
HU Yonghua1 2 ZHOU Yuan2 YAN Chongling2 LIU Junmin2 ZHANG Weiyou2 & WANG Qin2?
1 School of Biological Science and Biotechnology, Minnan Normal University, Zhangzhou 363000, China 2 Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen 361102, China
关键词:
铅胁迫双孢蘑菇积累抗氧化酶
Keywords:
lead stress Agaricus bisporus accumulation antioxidant enzyme
DOI:
10.19675/j.cnki.1006-687x.2019.05041
摘要:
大型真菌具有强的金属富集能力及较高的相对年生物量,因此在对铅(Pb)污染的修复中有一定优势. 以双孢蘑菇菌丝为研究对象,采用马铃薯葡萄糖琼脂培养的方法,研究不同浓度Pb胁迫对双孢蘑菇菌丝体的生长状况、形态以及机体内抗氧化酶系统的影响. 结果表明,在试验浓度下Pb对双孢蘑菇菌丝体的生长率无显著性影响,且菌丝体中Pb含量随着培养基中Pb浓度的增加而升高,达到10.40 μg/g. 扫描电镜和透射电镜结果显示,Pb胁迫下菌丝体的细胞结构受到一定破坏,细胞膜透性增加. X射线能谱分析观察得到细胞中的黑色颗粒为铅,表明Pb在细胞内的富集. 菌丝体中丙二醛含量随Pb浓度的增加而增加,超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)等抗氧化酶活性随着Pb浓度的增加有不同程度的反应. 在酶活力达到最大时,相比对照组分别增加了56.45%、61.36%、13.08%和10.59%. 本研究表明双孢蘑菇通过调节自身抗氧化酶系统适应Pb胁迫环境,在Pb污染的生物修复中具有应用潜力. (图5 参30)
Abstract:
Lead (Pb) is one of the most common heavy metal pollutants in the environment. It not only directly poisons soil organisms and destroys the ecosystem structure, but it can also migrate and transform through the food chain and harm human health. Therefore, the treatment of Pb pollution is extremely urgent. Owing to their strong metal enrichment ability and high relative annual biomass, macrofungi have certain advantages for the remediation of heavy metal pollution. In the present study, the mycelium of Agaricus bisporus was exposed to different Pb concentrations to evaluate its growth, morphology, and activity of antioxidant enzymes. The results showed that for the experimental Pb concentrations applied, mycelium growth had no significant effect and the accumulation of Pb increased in a concentration-dependent manner to 10.40 μg/g. The results from the scanning and transmission electron microscopes showed that the cell structure of mycelium was damaged, and the membrane permeability was increased under Pb treatment. The black particles present in the cells were identified as Pb from the X-ray energy dispersive microanalysis, which indicated that Pb accumulated in the cells. Malondialdehyde content increased with increasing Pb concentration. The activities of antioxidant enzymes such as superoxide dismutase, peroxidase, catalase, and ascorbate peroxidase had different degrees of response with increasing Pb concentrations. When the enzyme activity reached its maximum, these activities increased by 56.45%, 61.36%, 13.08%, and 10.59%, respectively, compared with the control. This study showed that A. bisporus can adjust its own antioxidant enzyme system to adapt to Pb stress, suggesting that A. bisporus has application potential for Pb remediation.

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更新日期/Last Update: 2020-04-25