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[1]孙悦,胡渤洋,徐鑫,等.二年残孔菌的培养条件优化及其液体发酵产物的抗氧化及漆酶活性[J].应用与环境生物学报,2020,26(02):306-311.[doi:10.19675/j.cnki.1006-687x.2019.05039]
 SUN Yue,HU Boyang,XU Xin,et al.Culture condition optimization, antioxidant capacity, and laccase production of Abortiporus biennis[J].Chinese Journal of Applied & Environmental Biology,2020,26(02):306-311.[doi:10.19675/j.cnki.1006-687x.2019.05039]
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二年残孔菌的培养条件优化及其液体发酵产物的抗氧化及漆酶活性
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
26卷
期数:
2020年02期
页码:
306-311
栏目:
研究论文
出版日期:
2020-04-25

文章信息/Info

Title:
Culture condition optimization, antioxidant capacity, and laccase production of Abortiporus biennis
作者:
孙悦胡渤洋徐鑫刘子璐李小凤张国庆
1北京农学院生物与资源环境学院,农业农村部华北都市农业重点实验室 北京 102206 2北京农学院植物科学技术学院,北京林木分子设计育种高精尖创新中心,农业应用新技术北京市重点实验室 北京 102206
Author(s):
SUN Yue1 HU Boyang1 XU Xin1 LIU Zilu1 LI Xiaofeng1 & ZHANG Guoqing1 2?
1 College of Bioscience and Resource Environment, Beijing University of Agriculture, Key Laboratory of Urban Agriculture (North China) of Ministry of Agriculture and Rural Affairs, Beijing 102206, China 2 College of Plant Science and Technology, Beijing University of Agriculture, Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Key Laboratory for Agricultural Application and New Technique, Beijing 102206, China
关键词:
二年残孔菌培养条件抗氧化活性漆酶
Keywords:
Abortiporus biennis culture condition antioxidant activity laccase
DOI:
10.19675/j.cnki.1006-687x.2019.05039
摘要:
二年残孔菌(Abortiporus biennis)是一种具有抗氧化、抗肿瘤功效的药用白腐真菌,可降解木质素、造成木材白色腐朽. 以分离自江苏无锡的二年残孔菌WX-02菌株为材料,开展菌丝最适培养条件、液体发酵产物抗氧化活性及Cu2+诱导产漆酶活性研究. 基于单因子试验的最适生长条件结果表明,葡萄糖是WX-02菌株菌丝体生长的最适碳源,牛肉膏和黄豆粉为最适氮源,C/N比为40/1时长势最佳,玉米粉和VC为最适生长因子,而菌丝生长最适pH和温度分别为7.0和32 ℃ 10%(V/V)接种量、28 ℃、150 r/min培养72 h条件下,PD培养基发酵液的总抗氧化活性为2.76 ± 0.31 mmol/L(FeSO4)和0.40 ± 0.01 mmol/L(Trolox),DPPH自由基清除率为100.00% ± 0.00%,总SOD活力为15.75 ± 最适培养条件液体培养基发酵液的总抗氧化活性为0.20 ± 0.03 mmol/L(FeSO4)和0.16 ± 0.01 mmol/L(Trolox),DPPH自由基清除率为59.06% ± 0.61%,总SOD活力为15.65 ± 0.62 U/mL. 0.25 mmol/L Cu2+对胞外漆酶诱导效果最佳,PD培养基、0.25 mmol/L Cu2+、28 ℃、150 r/min培养96 h,胞外漆酶活性最高,为(1.82 ± 0.02)× 105 U/mL,是对照组的1.72倍. 本研究表明WX-02菌株具有良好的抗氧化和产漆酶活性,具有药用和环境治理开发应用潜能. (图4 表4 参34)
Abstract:
Abortiporus biennis is a medicinal white rot fungus with antioxidation and anti-tumor effects, which can degrade lignin and cause white decay of wood. In the present study, A. biennis strain WX-02 isolated from Wuxi, Jiangsu Province, was used as the study material. The optimal culture conditions for mycelia, antioxidant activities, and Cu2+-induced laccase production of liquid fermentation were investigated. Culture conditions based on single factor experiments revealed that the optimum carbon source for strain WX-02 mycelial growth was glucose, whereas the optimum nitrogen sources were beef extract and soybean powder. When the C/N ratio was 40/1, the mycelia demonstrated the highest growth vigor. The optimum growth factors were corn flour and vitamin C, and the optimal pH and temperature were 7.0 and 32 ℃ respectively. With a 10% (V/V) inoculation concentration at 28 ℃ and 150 r/min for 72 h, the fermentation broth using PD medium manifested a total antioxidant activity of 2.76 ± 0.31 mmol/L (FeSO4) and 0.40 ± 0.01 mmol/L (Trolox), DPPH radical scavenging ratio of 100.00 ± 0.00%, and SOD activity of 15.75 ± 0.25 U/mL. The fermentation broth using the optimum culture condition medium demonstrated a total antioxidant activity of 0.20 ± 0.03 mmol/L (FeSO4) and 0.16 ± 0.01 mmol/L (Trolox), DPPH radical scavenging ratio of 59.06 ± 0.61%, and SOD activity of 15.65 ± 0.62 U/mL. Further study of Cu2+ on extracellular laccase production revealed that 0.25 mmol/L of Cu2+ significantly enhanced the enzyme production with the highest activity of 1.82 ± 0.02 × 105 U/mL after liquid fermentation at 28 ℃ at 150 r/min for 96 h, which was 1.72 times higher than that of the control group. The WX-02 strain demonstrated considerably high antioxidant and laccase activities, suggesting its potential application in medicinal and environmental management.

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更新日期/Last Update: 2020-04-25