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[1]顾李成,黄彩怡,黄淑芬,等.土壤栽培和无土栽培菜心中内生细菌比较及分离鉴定[J].应用与环境生物学报,2019,25(06):1420-1427.[doi:10.19675/j.cnki.1006-687x.2019.0305]
 GU Licheng,HUANG Caiyi,HUANG Shufen,et al.Comparison, identification, and characterization of endophytic bacteria from Chinese flowering cabbage (Brassica parachinensis) using soil and soilless culture[J].Chinese Journal of Applied & Environmental Biology,2019,25(06):1420-1427.[doi:10.19675/j.cnki.1006-687x.2019.0305]
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土壤栽培和无土栽培菜心中内生细菌比较及分离鉴定
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年06期
页码:
1420-1427
栏目:
研究论文
出版日期:
2019-12-30

文章信息/Info

Title:
Comparison, identification, and characterization of endophytic bacteria from Chinese flowering cabbage (Brassica parachinensis) using soil and soilless culture
作者:
顾李成黄彩怡黄淑芬江宝茹谭淑雅陈俊毅彭桂香谭志远
1华南农业大学农学院 广州 510642 2华南农业大学资源环境学院 广州 510642
Author(s):
GU Licheng1 HUANG Caiyi1 HUANG Shufen1 JIANG Baoru1 TAN Shuya1 CHEN Junyi1 PENG Guixiang2 & TAN Zhiyuan1**
1 College of Agriculture, South China Agricultural University, Guangzhou 510642, China 2 College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China
关键词:
无土栽培土壤栽培菜心内生细菌相对叶绿素含量可溶性糖含量
Keywords:
soilless culture soil culture Brassica parachinensis endophytic bacteria relative chlorophyll content (SPAD) soluble sugar content
分类号:
Q939.11 : S634.5
DOI:
10.19675/j.cnki.1006-687x.2019.0305
摘要:
菜心(Brassica parachinensis)是华南地区的一种特色蔬菜. 为探究无土栽培和土壤栽培菜心内生细菌的差异,以这两种栽培方式的菜心为材料,分离内生细菌. 根据插入序列指纹图谱(IS-PCR)结果进行聚类,对代表性菌株的16S rRNA基因序列进行系统发育分析,并将菌株加入无土栽培营养液中,收获后测定菜心的的相对叶绿素含量(SPAD)和可溶性糖含量. 结果显示,从两种菜心中共分离到31株内生细菌,IS-PCR结果将其聚为9个类群,16S rRNA基因鉴定其分别属于Bacillus sp.(芽孢杆菌属)、Rhizobium sp.(根瘤菌属)、Microbacterium sp.(微杆菌属)、Leifsonia sp.(雷夫松氏菌属)、Sphingomonas sp.(鞘氨醇单胞菌属)、Alcaligenes sp.(产碱菌属)和Agrobacterium sp.(农杆菌属). 土壤栽培菜心中可培养细菌数目多于无土栽培菜心,且土壤栽培菜心中内生细菌的碳代谢更多样化. 生理生化实验显示,部分菌株能分泌生长素和铁载体. 加入土壤栽培菜心中分离得到的菌株SCG14和SCG0802后,无土栽培菜心的相对叶绿素含量和可溶性糖含量比对照显著提高. 本研究表明无土栽培和土壤栽培的菜心中的内生细菌在种类、数目、分布部位和碳源利用能力上有差异;此外,分离得到的菌株能提高无土栽培菜心的相对叶绿素以及可溶性糖含量,有开发为微生物制剂的潜力. (图4 表6 参35)
Abstract:
Chinese flowering cabbage (Brassica parachinensis) is a type of special vegetable in southern China. In the present study, several endophytic bacterial strains were respectively isolated from soil and soilless cultivated Chinese flowering cabbage to identify the difference between the two materials. The isolated strains were divided into several groups according to their IS-PCR DNA fingerprinting patterns. Thereafter, 16S rRNA gene sequences of representative strains were aligned for phylogenetic analysis. The bacterial fluid of the representative strains was added into the soilless culture nutrient solution during planting. The relative chlorophyll content (SPAD) and soluble sugar content of each group were muesured after harvest. As a result, 31 isolates were respectively acquired from the two materials and were divided into 9 groups by the IS-PCR fingerprinting method. Through a subsequent analysis of the 16S rRNA gene sequences of the representative strains, they were implied to belong to Bacillus sp., Rhizobium sp., Microbacterium sp., Leifsonia sp., Sphingomonas sp., Alcaligenes sp., and Agrobacterium sp. By using the plate counting method, we confirmed the presence of more culturable endophytic bacteria inside the soil cultured than the soilless cultured Chinese flowering cabbage. Furthermore, the Bio-BIQA board test result indicated that carbon metabolism of microbes from the soil cultured Chinese flowering cabbage had more diversity than those from the soilless cultured cabbage. The physiological and biochemical tests revealed that some strains could secrete indole acetic acid and produce siderophore. After adding the bacterial fluid of the strains, SCG14 and SCG0802, into the soilless culture nutrient solution, the relative chlorophyll content and soluble sugar content of Chinese flowering cabbage caused a significant promotion relative to that recognized in the control group. These findings demonstrate that the endophytic bacteria in soilless cultured Chinese flowering cabbage and those in soil cultured Chinese flowering cabbage differ in type, number, distribution, and carbon metabolism. In addition, the isolated strains could enhance the relative chlorophyll content (SPAD) and soluble sugar content of soilless cultured Chinese flowering cabbage, which have a prospect in the development of new microbial preparations.

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更新日期/Last Update: 2019-12-25