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[1]王东梅,周敏,刘燕,等.聚花野丁香的化学成分及其抗氧化活性[J].应用与环境生物学报,2019,25(06):1492-1496.[doi:10.19675/j.cnki.1006-687x.2019.03049]
 WANG Dongmei,ZHOU Min,et al.Chemical components from Leptodermis glomerata and their antioxidant activity[J].Chinese Journal of Applied & Environmental Biology,2019,25(06):1492-1496.[doi:10.19675/j.cnki.1006-687x.2019.03049]
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聚花野丁香的化学成分及其抗氧化活性
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年06期
页码:
1492-1496
栏目:
研究论文
出版日期:
2019-12-30

文章信息/Info

Title:
Chemical components from Leptodermis glomerata and their antioxidant activity
作者:
王东梅周敏刘燕戚华溢张国林罗应刚
1中国科学院成都生物研究所 成都 610041 2中国科学院大学 北京 100049
Author(s):
WANG Dongmei1 2 ZHOU Min1 LIU Yan1 QI Huayi1 ZHANG Guolin1 & LUO Yinggang1**
1 Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China 2 University of Chinese Academy of Sciences, Beijing 100049, China
关键词:
聚花野丁香杨梅素蒽醌抗氧化活性
Keywords:
Leptodermis glomerata myricetin anthraquinone antioxidant activity
分类号:
R284.1
DOI:
10.19675/j.cnki.1006-687x.2019.03049
摘要:
为研究聚花野丁香(Leptodermis glomerata)的生物活性,综合利用多种色谱方法,对该植物95%乙醇提取物的化学成分进行了分离、纯化,从中共得到15个化合物. 通过波谱数据分析及比对,将其分别鉴定为杨梅素(1)、(2R,3R)-二氢杨梅素(2)、槲皮素(3)、pinostrobin(4)、1,3-二甲氧基-2-羟基蒽醌(5)、2-甲氧基-1,3-二羟基蒽醌(6)、1,3,4-三甲氧基-2-羟基蒽醌(7)、1,2,3,4-四甲氧基蒽醌(8)、1,2,3-三甲氧基蒽醌(9)、5,7-二羟基色原酮(10)、3S-faramol(11)、(2R,4S)-catalponol(12)、(2S,4S)-catalponol(13)、3β-乙酰基齐墩果酸(14)、齐墩果酸(15). 采用DPPH、ABTS、NO三种自由基清除活性测试模型,对上述化合物的抗氧化活性进行测试. 结果表明,(2R,3R)-二氢杨梅素(2)、pinostrobin(4)和5,7-二羟基色原酮(10)表现出显著的自由基清除活性:当浓度为1 mg/mL时,化合物2对DPPH、NO自由基的清除率为91.2%、67.8%;当浓度为2 mg/mL时,化合物4对DPPH自由基的清除率为102.8%;当浓度为1 mg/mL时,化合物10对ABTS、NO自由基的清除率分别为104.7%、95.7%. 本研究表明聚花野丁香富含多羟基酚类化合物,其中部分化合物显示较强的抗氧化活性,具有开发利用价值. (图1 表1 参23)
Abstract:
Fifteen natural products were isolated from the aqueous ethanol extract of Leptodermis glomerata using various chromatographic methods. Based on spectrum data, these compounds were identified as follows: myricetin (1), (2R,3R)-dihydromyricetin (2), quercetin (3), pinostrobin (4), 1,3-demethoxy-2-hydroxyanthraquinone (5), 2-methoxy-1,3-dehydroxyanthraquinone (6), 1,3,4-trimethoxy-2-hydroxyanthraquinone (7), 1,2,3,4-tetramethoxy anthraquinone (8), 1,2,3-trimethoxyanthraquinone (9), 5,7-dihydroxyprimary ketone (10), 3S-faramol (11), (2R, 4S)-catalponol (12), (2S,4S)-catalponol (13), 3β-acetyloleanolic acid (14), and oleanolic acid (15). (2R,3R)-Dihydromyricetin (2), pinostrobin (4), and 5,7-dihydroxyprimaryketone (10) exhibited significant radical scavenging effects according to the results of 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), and nitric oxide (NO) activity assays. Compound 2 (1 mg/mL) showed 91.2% and 67.8% radical scavenging activities against DPPH and NO radicals, respectively. Compound 4 (2 mg/mL) showed 102.8% DPPH radical scavenging activity. Compound 10 (1 mg/mL) was able to scavenge ABTS and NO radicals with 104.7% and 95.7% radical scavenging activity, respectively.

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更新日期/Last Update: 2019-12-25