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[1]侯颖,裴韬,王维宇,等.一株氰氟草酯降解新菌株Sphingopyxis sp. WP9的分离鉴定及降解特性[J].应用与环境生物学报,2019,25(06):1464-1469.[doi:10.19675/j.cnki.1006-687x.2019.03005]
 HOU Ying**,PEI Tao,WANG Weiyu,et al.Isolation, identification, and characterization of a new cyhalofop-butyl (CyB)-degrading strain, Sphingopyxis sp. WP9[J].Chinese Journal of Applied & Environmental Biology,2019,25(06):1464-1469.[doi:10.19675/j.cnki.1006-687x.2019.03005]
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一株氰氟草酯降解新菌株Sphingopyxis sp. WP9的分离鉴定及降解特性
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年06期
页码:
1464-1469
栏目:
研究论文
出版日期:
2019-12-30

文章信息/Info

Title:
Isolation, identification, and characterization of a new cyhalofop-butyl (CyB)-degrading strain, Sphingopyxis sp. WP9
作者:
侯颖裴韬王维宇尤晓颜牛明福黄天晨
河南科技大学食品与生物工程学院 洛阳 471023
Author(s):
HOU Ying** PEI Tao WANG Weiyu YOU Xiaoyan NIU Mingfu** & HUANG Tianchen
College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China
关键词:
氰氟草酯鞘氨醇盒菌分离鉴定降解特性
Keywords:
cyhalofop-butyl Sphingopyxis isolation and identification degradation characteristics
分类号:
X172
DOI:
10.19675/j.cnki.1006-687x.2019.03005
摘要:
氰氟草酯是应用于稻田的一种除草剂,其广泛使用给水生生态带来了一定威胁. 利用连续富集培养的方法,从农药厂活性污泥中筛选出一株氰氟草酯高效降解菌WP9. 采用16S rRNA基因序列分析并结合菌落菌体形态和生理生化特性,将菌株WP9鉴定为鞘氨醇盒菌(Sphingopyxis). 在以100 mg/L氰氟草酯为唯一碳源的无机盐培养基中,菌株WP9在120 h内可将其降解97.03%. 菌株WP9降解炔草酯的最适温度和pH分别为30 ℃和7.0;当菌株WP9的接种量≥ 5%时,其对100 mg/L氰氟草酯的降解效果较好;当氰氟草酯浓度≤100 mg/L时,菌株WP9对氰氟草酯的平均降解速率随着底物浓度的增加而呈指数增长. 通过超高效液相色谱-质谱联用技术(UPLC-MS)鉴定降解产物为氰氟草酸和氰氟草酚,推测氰氟草酯首先通过酯键水解生成氰氟草酸,然后氰氟草酸通过醚键水解生成氰氟草酚. 本研究关于鞘氨醇盒菌对氰氟草酯降解的报道结果,可为开展氰氟草酯污染环境的生物修复提供新菌种和理论基础. (图8 参30)
Abstract:
Cyhalofop-butyl (CyB) is a herbicide that is often applied in rice paddy fields; however, its widespread agricultural use poses a threat to the aquatic ecology. An efficient CyB-degrading strain, WP9, was isolated from the active sludge from a pesticide factory via continuous enrichment and cultivation. Strain WP9 was identified as Sphingopyxis via 16S rRNA gene sequencing as well as its morphological, physiological and biochemical characteristics. Strain WP9 could degrade 97.03% of 100 mg/L CyB in inorganic salt medium CyB was used as the sole carbon source. The optimum temperature and pH for the degradation of CyB were 37 ℃ and 8.0, respectively. When the concentration of CyB ≤ 100 mg/L, the average degradation rate of CyB by strain WP9 increased exponentially as the substrate concentration increased. When the inoculation size of strain WP9 was ≥ 5%, the degradation of 100 mg/L CyB by strain WP9 was efficient. The metabolites of cyB were identified as cyhalofop acid (CyA) and cyhalofop phenol by Ultra-performance Liquid Chromatography - Mass Spectrometry (UPLC-MS) analysis. We proposed the following possible mechanism of CyB degradations as follows: CyB was transformed to CyA quickly via hydrolysis of the ester bond, and then CyA was converted to cyhalofop phenol. This study reports the biological degradation of CyB by Sphingopyxis and it provides a new strain and potential method of removing pesticide pollution from the environment.

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相似文献/References:

[1]聂志娟,顾谢军,蔡舒,等.一株除草剂氰氟草酯降解菌的分离鉴定与降解特性[J].应用与环境生物学报,2011,17(06):869.[doi:10.3724/SP.J.1145.2011.00869]
 NIE Zhijuan,GU Xiejun,CAI Shu,et al.Isolation of a Cyhalofop-butyl-degrading Bacterium and Its Degradation Characteristics[J].Chinese Journal of Applied & Environmental Biology,2011,17(06):869.[doi:10.3724/SP.J.1145.2011.00869]

更新日期/Last Update: 2019-12-25