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[1]吴玲.富营养化太湖沉积物中微生物群落及对环境因子的响应[J].应用与环境生物学报,2019,25(06):1470-1476.[doi:10.19675/j.cnki.1006-687x.2019.02007]
 WU Ling,**.Response of microbial community structure in eutrophic Taihu sediments to environmental factors[J].Chinese Journal of Applied & Environmental Biology,2019,25(06):1470-1476.[doi:10.19675/j.cnki.1006-687x.2019.02007]
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富营养化太湖沉积物中微生物群落及对环境因子的响应
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年06期
页码:
1470-1476
栏目:
研究论文
出版日期:
2019-12-30

文章信息/Info

Title:
Response of microbial community structure in eutrophic Taihu sediments to environmental factors
作者:
吴玲
1常州工程职业技术学院制药与环境工程学院 常州 213164 2南京师范大学地理科学学院,江苏省物质循环与污染控制重点实验室 南京 210023
Author(s):
WU Ling1 2**
1 Institute of Pharmaceutical and Environmental Engineering, Changzhou Vocational Institute of Engineering, Changzhou 213164, China 2 Jiangsu Provincial Key Laboratory of Materials Cycling and Pollution Control, School of Geography Sciences, Nanjing Normal University, Nanjing 210023, China
关键词:
微生物多样性宏基因组测序技术富营养太湖沉积物环境因子
Keywords:
microbial diversity metagenomic sequencing technology eutrophic Taihu Lake sediment environmental factor
分类号:
X826 : X524
DOI:
10.19675/j.cnki.1006-687x.2019.02007
摘要:
沉积物是水生态系统重要的组成部分,研究沉积物中的微生物多样性及群落结构有助于从侧面了解水体的水质状况. 采集太湖中具有不同富营养化水平的梅梁湾(ML)与湖心区(HX)表层沉积物(0 - -2 cm),测定沉积物样品中的总有机碳(TOC)、总氮(TN)、无机氮(NH4+-N与NO3--N)、pH、氧化还原电位(Eh)与溶解氧(DO),利用基于16S rRNA基因的Illumina Miseq宏基因组测序技术研究沉积物样品中的微生物群落结构,并分析沉积物中微生物群落结构与环境因子的潜在关系. 从太湖两处采样点6个沉积物样品中共获得234 408条有效序列,Sobs指数在1 811-2 442之间,Shannon指数在6.16-6.49之间,Coverage值在96.2%-97.7%之间,说明序列信息量足够大且微生物多样性较高. 沉积物中细菌相对丰度为99%以上,共检测到48个门118个纲. 其中变形菌门(Proteobacteria)为优势菌门,在ML和HX沉积物中的相对丰度分别为40.5%和35.4%,主要包括δ-proteobacteria和β-proteobacteria纲. 其他较为丰富的门类包括绿弯菌门(Chloroflexi)、硝化螺菌门(Nitrospirae)和拟杆菌门(Bacteroidetes)等. 将沉积物中纲水平微生物群落组成与理化因子进行Spearman相关分析可知,Eh、DO、pH、NH4+-N和TOC含量能显著影响纲水平优势微生物类群(OTU > 1%),且以Eh影响的微生物类群种类最多. 上述研究表明,太湖沉积物中微生物资源丰富,不同富营养湖区理化因子是影响其中微生物群落结构的重要因素. (图4 表3 参42)
Abstract:
Sediment is an important component of the aquatic ecosystem, and the study of microbial diversity in the sediment is an important method of evaluating water quality. This paper aimed to study the structure of the microbial community in the sediment from areas of Taihu Lake (China) with different levels of eutrophication as well as the effects of main physicochemical indices on bacterial community structures. The surface sediments (0 - –2 cm) were collected from Meiliang Bay (ML) and Huxin (HX) of Taihu Lake. The total organic carbon (TOC), total nitrogen (TN), inorganic nitrogen (NH4+-N and NO3--N), pH, redox potential (Eh), and dissolved oxygen (DO) were measured. Illumina Miseq metagenomic sequencing technology was used to analyze the microbial community structure in different sediment samples, and the potential relationship between microbial community structure and physicochemical factors was also studied. A total of 234 408 valid reads from six sediment samples were obtained, with a Sobs index of 1 811-2 442, a Shannon index of 6.16-6.49, and coverage between 96.2% and 97.7%. These results suggested sufficient sequencing data and a high level of community diversity. The relative abundance of bacterial species in the sediment from sampling areas of Taihu Lake contributed to more than 99% of the total sequences, which included a total of 48 phyla and 113 classes. The total number of reads in the six sediment samples was mainly affiliated with proteobacteria, which were dominated by two classes of δ-proteobacteria and β-proteobacteria. The relative proportions of proteobacteria in ML and HX were 40.5% and 35.4%, respectively. Other abundant bacterial groups include species from the phyla Chloroflexi, Nitrospirae, and Bacteroidetes. We also found that the Eh, DO, pH, ammonium nitrogen, and TOC values can significantly affect the number of bacterial classes (OTU > 1%). Among these factors, Eh is the factor that affects the largest number of microbial groups based on the Spearman correlation analyses of microbial community structures and the physiochemical indices of sediments. Studies showed that there are abundant microbial resources in sediment samples from Taihu Lake, and the physiochemical indices from different eutrophic areas are important factors affecting the microbial community structure.

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更新日期/Last Update: 2019-12-25