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[1]王祎,杨文浩,毛艳玲,等.水稻生育期对不同施肥条件下黄泥田土壤无机氮及细菌群落的影响[J].应用与环境生物学报,2019,25(06):1352-1358.[doi:10.19675/j.cnki.1006-687x.2019.01046]
 WANG Yi,YANG Wenhao,et al.Effect of the phenological stage of rice growth on soil-soluble inorganic nitrogen and bacterial communities in a yellow clayey soil under different fertilization patterns[J].Chinese Journal of Applied & Environmental Biology,2019,25(06):1352-1358.[doi:10.19675/j.cnki.1006-687x.2019.01046]
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水稻生育期对不同施肥条件下黄泥田土壤无机氮及细菌群落的影响
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年06期
页码:
1352-1358
栏目:
研究论文
出版日期:
2019-12-30

文章信息/Info

Title:
Effect of the phenological stage of rice growth on soil-soluble inorganic nitrogen and bacterial communities in a yellow clayey soil under different fertilization patterns
作者:
王祎杨文浩毛艳玲周碧青聂三安邢世和
1福建农林大学资源与环境学院 福州 350002 2福建农林大学土壤生态系统健康与调控福建省高校重点实验室 福州 350002 3福建农林大学生命科学学院 福州 350002
Author(s):
WANG Yi1 2 YANG Wenhao1 2 MAO Yanling1 2 ZHOU Biqing1 2 NIE San’an1 3 & XING Shihe1 2**
1 College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China 2 Key Laboratory of Soil Ecosystem Health and Regulation, Fujian Agriculture and Forestry University, Fuzhou 350002, China 3 College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
关键词:
施肥模式水稻生育期土壤细菌可溶性无机氮
Keywords:
fertilization pattern rice growth stage soil bacteria soluble inorganic nitrogen
分类号:
S154.3
DOI:
10.19675/j.cnki.1006-687x.2019.01046
摘要:
为揭示不同施肥条件下水稻生育期黄泥田土壤细菌群落的动态变化及对可溶性无机氮的影响,以33年不同施肥处理(不施肥CK、单施无机肥NPK、无机肥配施牛粪NPKM和无机肥配施秸秆NPKS)的黄泥田为研究对象,采用实时荧光定量PCR和高通量测序等技术分析水稻幼苗期、分蘖期、拔节期、扬花期和成熟期的黄泥田耕层土壤细菌数量、群落组成和结构. 结果显示,黄泥田可溶性无机氮以铵态氮为主,含量为6.01-30.93 mg/kg,占可溶性无机氮总量的95%以上. 细菌16S rRNA基因拷贝数为3.03 × 107 - 14.33 × 107/g干土. 土壤优势细菌群落为变形菌门、绿弯菌门、放线菌门和酸杆菌门. 偏最小二乘法判别分析表明,不同施肥处理细菌群落差异以拔节期最为明显;不同生育期细菌群落差异以无机有机肥配施处理最为明显. 多元方差分析表明,水稻生育期可以解释细菌群落结构59.79%的变异,施肥处理可以解释细菌群落结构10.44%的变异,土壤铵态氮与细菌16S rRNA基因拷贝数呈显著正相关(P < 0.05). 本研究表明土壤细菌群落与土壤铵态氮存在一定的相关性,且水稻不同生育期引起的土壤细菌群落结构差异比不同施肥处理引起的细菌群落结构差异更为明显. (图3 表4 参26)
Abstract:
The aim of this study was to reveal the dynamics of soil bacterial communities and their effects on the levels of soil-soluble inorganic nitrogen in paddy soil during the growth period of rice under different fertilization patterns. A yellow clayey paddy soil in the Fujian province with 33 years’ worth of different fertilization treatments including control (CK), chemical fertilizer (NPK), chemical fertilizer combined with cow manure (NPKM), and chemical fertilizer combined with rice straw (NPKS), was used in this study. The abundance and diversity of bacterial species, bacterial community structure, and levels of soil-soluble inorganic nitrogen in the paddy field during the seedling, tilling, booting, flowering and ripening stages were analyzed by real-time quantitative PCR and high-throughput sequencing. The results showed that the concentration of ammonium in the yellow clayey paddy soil ranged from 6.01 to 30.93 mg/kg and was the main source of soluble inorganic nitrogen in the soil. The 16S rRNA gene copy number ranged from 3.03 × 107 to 14.33 × 107 per g of dry soil, which showed that the abundance of soil bacteria varied greatly during the rice growth stages. The dominant bacterial communities in yellow clayey paddy soil were Proteobacteria, Chloroflexi, Actinobacteria, and Acidobacteria. Partial Least Squares Discriminant Analyses (PLS-DA) showed that the booting stage induces great variation in bacteria communities compared with other growth stages of rice. Also, inorganic fertilizers combined with organic fertilizers (NPKS and NPKM) showed great variation in comparison to different treatments. The permutational multivariate analysis of variance (PERMANOVA) results indicated that the rice growth stages accounted for 59.79% of the variation in the structure of the bacterial community while the treatment explained the 10.44% variation in the bacterial community structure. The soil ammonium concentration was positively correlated with the abundance of bacteria (P < 0.05). We concluded that the structure of the bacterial community in the soil was related to the concentration of ammonium nitrogen in paddy soil. Differences in the bacterial community structure were more likely to be influenced by the growth stages of rice rather than fertilization patterns.

参考文献/References:

1. Fierer N. Embracing the unknown: disentangling the complexities of the soil microbiome [J]. Nat Rev Microb, 2017, 15 (10): 579-590
2. 张立成, 肖卫华, 彭沛宇, 廖健程, 丁鑫, 胡德勇. 稻-稻-油菜轮作土壤细菌群落的特征[J]. 应用与环境生物学报, 2018, 24 (2): 1-9 [Zhang CL, Xiao WH, Peng PY, Liao JC, Ding X, Hu DY. Soil bacterial community characteristics of rice-rice-rape crop rotation [J]. Chin J Appl Environ Biol, 2018, 24 (2): 1-9]
3. Geisseler D, Horwath WR, Joergensen RG, Ludwig B. Pathways of nitrogen utilization by soil microorganisms - a review [J]. Soil Biol Biochem, 2010, 42 (12): 2058-2067
4. Geisseler D, Linquist BA, Lazicki PA. Effect of fertilization on soil microorganisms in paddy rice systems – a meta-analysis [J]. Soil Biol Biochem, 2017, 115: 452-460
5. Guo J, Liu W, Zhu C, Luo, Gong W, Kong YL, Ling N, Wang M, Dai JY, Shen QR, Guo SW. Bacterial rather than fungal community composition is associated with microbial activities and nutrient-use efficiencies in a paddy soil with short-term organic amendments [J]. Plant Soil, 2018, 424 (1): 335-349
6. Kumar U, Nayak AK, Shahid M, Gupta VVSR, Panneerselvam P, Mohanty S, Kaviraj M, Kumar A, Chatterjee D, Lal B, Gautam P, Tripathi R, Panda BB. Continuous application of inorganic and organic fertilizers over 47 years in paddy soil alters the bacterial community structure and its influence on rice production [J]. Agric Ecosyst Environ, 2018, 262: 65-75
7. Zhai YM, Hou MM, Nie SA. Variance of microbial composition and structure and relation with soil properties in rhizospheric and non-rhizospheric soil of a flooded paddy [J]. Paddy Water Environ, 2018, 16 (1): 163-172
8. Bossio DA, Scow KM, Gunapala N, Graham KJ. Determinants of soil microbial communities: effects of agricultural management, season, and soil type on phospholipid fatty acid profiles [J]. Microb Ecol, 1998, 36 (1): 1-12
9. Luo X, Han S, Fu X, Li X, Wang L, Peng SB, Chen WL, Huang QY. The microbial network in naturally fertile paddy soil possibly facilitates functional recruitment in the rice mature stage [J]. Appl Soil Ecol, 2019, 135: 174-181
10. Li H, Su JQ, Yang XR, Zhu YG. Distinct rhizosphere effect on active and total bacterial communities in paddy soils [J]. Sci Total Environm, 2019, 649: 422-430
11. 吴朝晖, 刘清术, 孙继民, 周建群, 李鸿波, 袁隆平. 基于高通量测序的超级稻不同生育期土壤细菌和古菌群落动态变化[J]. 农业现代化研究, 2018, 39 (2): 342-351 [Wu ZH, Liu QS, Sun JM, Zhou JQ, Li HB, Yuan LP, Variations of soil bacterial and archaeal communities during super hybrid rice cultivation based on high throughput sequencing [J]. Res Agric Mod, 2018, 39 (2): 342-351]
12. Breidenbach B, Pump J, Dumont MG. Microbial community structure in the rhizosphere of rice plants [J]. Front Microbiol, 2016, 6 (1537): 1-12
13. 袁红朝, 吴昊, 葛体达, 李科林, 吴金水, 王久荣. 长期施肥对稻田土壤细菌、古菌多样性和群落结构的影响[J]. 应用生态学报, 2015, 26 (6): 1807-1813 [Yuan HZ, Wu H, Ge TD, Li KL, Wu JS, Wang JR [J]. Chin J Appl Ecol, 2015, 26 (6): 1807-1813]
14. Nie SA, Lei XM, Zhao LX, Brookes PC, Wang F, Chen CR, Yang WH, Xing SH. Fungal communities and functions response to long-term fertilization in paddy soils [J]. Appl Soil Ecol, 2018, 130: 251-258
15. 佘冬立, 王凯荣, 谢小立, 彭英湘, 陈敏. 稻草还田与施氮水平对土壤氮素供应和水稻产量的影响[J]. 土壤通报, 2007, 38 (2): 296-300 [She DL, Wang KR, Xie XL, Peng YX, Chen M. Impact of rice straw incorporation and N rates on soil nitrogen supply and rice yield [J]. Chin J Soil Sci, 2007, 38 (2): 296-300]
16. Ramanathan KM, Krishnamoorthy KK. Nutrient uptake by paddy during the main three stages of growth [J]. Plant Soil, 1973, 39 (1): 29-33
17. Geisseler D, Linguist BA, Lazicki PA. Effect of fertilization on soil microorganisms in paddy rice systems - A meta-analysis [J]. Soil Biol Biochem, 2017, 115: 452-460
18. 杨静, 聂三安, 杨文浩, 陈成榕, 张黎明, 周碧青, 邢世和. 不同施肥水稻土可溶性有机氮组分差异及影响因素[J]. 土壤学报, 2018, 55 (4): 955-966 [Yang J, Nie SA, Yang WH, Chen CR, Zhang LM, Zhou BQ, Xing SH. Effects of various influencing factors of soil soluble organic nitrogen components under different long-term fertilization treatments in paddy soil [J]. Acta Pedol Sin, 2018, 55 (4): 955-966]
19. Yang CH, Crowley DE. Rhizosphere microbial community structure in relation to root location and plant iron nutritional status [J]. Appl Environ Microbiol, 2000, 66 (1): 345-351
20. Li H, Yang XR, Weng BS, Su JQ, Nie SA, Gilbert JA, Zhu YG. The phenological stage of rice growth determines anaerobic ammonium oxidation activity in rhizosphere soil [J]. Soil Biol Biochem, 2016, 100: 59-65
21. Yu HL, Ling N, Wang TT, Zhu C, Wang Y, Wang SJ, Gao Q. Responses of soil biological traits and bacterial communities to nitrogen fertilization mediate maize yields across three soil types [J]. Soil Tilla Res, 2019, 185: 61-69
22. Carson CM, Zeglin LH. Long-term fire management history affects N-fertilization sensitivity, but not seasonality, of grassland soil microbial communities [J]. Soil Biol Biochem, 2018, 121: 231-239
23. Sun YP, Guan YT, Wang HY, Wu GX. Autotrophic nitrogen removal in combined nitritation and Anammox systems through intermittent aeration and possible microbial interactions by quorum sensing analysis [J]. Biores Technol, 2019, 272: 146-155
24. Fierer N, Lauber CL, Ramirez KS, Zaneveld J, Bradford MA, Knight R. Comparative metagenomic, phylogenetic and physiological analyses of soil microbial communities across nitrogen gradients [J]. ISME J, 2011, 6 (5): 1007–1017
25. Zhao FZ, Bai L, Wang JY, Deng J, Ren CJ, Han XH, Yang GH, Wang J. Change in soil bacterial community during secondary succession depend on plant and soil characteristics [J]. Catena, 2019, 173: 246-252
26. Jiménez-Bueno NG, Valenzuela-Encinas C, Marsch R, Ortiz-Gutiérrez D, Verhulst N, Govaerts B, Dendooven L, Navarro-Noya YE. Bacterial indicator taxa in soils under different long-term agricultural management [J]. J Appl Microbiol, 2016, 120 (4): 921-933
27.

更新日期/Last Update: 2019-12-25