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[1]史楠,史小可,Jovliqizi Azimova Bahtigul,等.Eryxin通过抑制芳香化酶表达调控雌激素生物合成[J].应用与环境生物学报,2019,25(05):1205-1210.[doi:10.19675/j.cnki.1006-687x.2019.01028]
 SHI Nan,SHI Xiaoke,et al.Eryxin inhibits estrogen biosynthesis by suppression of aromatase[J].Chinese Journal of Applied & Environmental Biology,2019,25(05):1205-1210.[doi:10.19675/j.cnki.1006-687x.2019.01028]
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Eryxin通过抑制芳香化酶表达调控雌激素生物合成
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年05期
页码:
1205-1210
栏目:
研究论文
出版日期:
2019-10-31

文章信息/Info

Title:
Eryxin inhibits estrogen biosynthesis by suppression of aromatase
作者:
史楠史小可Jovliqizi Azimova BahtigulAbduvaliev AnvarRakhmanov Alisher王飞
1中国科学院成都生物研究所 成都 610041 2塔什干医科大学 塔什干 10012 3塔什干药物研究所 塔什干 10012 4中国科学院大学 北京 100049
Author(s):
SHI Nan1 4 SHI Xiaoke1 4 Jovliqizi Azimova Bahtigul1 3 Abduvaliev Anvar2 Rakhmanov Alisher2 & WANG Fei1**
1 Chengdu Institute of Biology, Chinese academy of Sciences, Chengdu 610041, China 2 Tashkent Medical Academy, Tashkent 10012, Uzbekistan 3 Tashkent Pharmaceutical Institute, Tashkent 10012, Uzbekistan 4 University of Chinese Academy of Sciences, Beijing 100049, China
关键词:
eryxin雌二醇芳香化酶卵巢颗粒细胞疾病
Keywords:
eryxin 17β-estradiol aromatase ovarian granulosa cell disease
分类号:
R961
DOI:
10.19675/j.cnki.1006-687x.2019.01028
摘要:
雌激素水平与女性生理及心理健康密切相关,为探讨eryxin对雌激素生物合成的影响,采用Am-blue法检测eryxin对人卵巢颗粒细胞KGN细胞增殖的影响,磁微粒分离酶联免疫法检测雌二醇的合成,Western blotting检测KGN细胞芳香化酶蛋白表达水平,RT-qPCR检测KGN细胞芳香化酶基因及其启动子Promoter Ⅱ表达水平. 结果显示,在1-150 μg/mL范围内eryxin对细胞增殖没有影响,但是可以显著抑制KGN细胞中雌二醇的合成,且具有剂量依赖性;eryxin对芳香化酶过表达的HEK-293A细胞中雌二醇的合成没有影响;eryxin显著下调芳香化酶的mRNA和蛋白质表达水平,抑制芳香化酶启动子Promoter Ⅱ表达;降低eryxin中蛋白质含量可显著削弱其抑制雌激素合成的活性. 本研究表明eryxin抑制人卵巢颗粒细胞雌激素的合成是通过其抑制芳香化酶mRNA及蛋白表达来实现的,结果可为进一步发现新型抗雌激素生物合成药物治疗相关相关疾病奠定基础. (图6 表1 参21)
Abstract:
Estrogen levels are a major factor affecting the physiological and mental health of women. The effect of eryxin on estrogen biosynthesis was investigated. The Am-blue method was used to detect the effect of eryxin on the proliferation of human ovarian granulosa (KGN) cells. The effects of eryxin on estrogen biosynthesis in KGN cells and aromatase-overexpressing HEK-293A (HEK-293Aaro) cells were examined by enzyme-linked immunosorbent assay (ELISA). The expression of aromatase protein in KGN cells were detected by Western blotting. We also examined the expression of aromatase gene and Promoter II by RT-qRCR. The results showed that 1-150 μg/mL of eryxin had no effect on cell proliferation but it potently inhibited the biosynthesis of 17β-estradiol in KGN cells in a dose-dependent manner; it had no effect on the catalytic activity of aromatase in HEK-293Aaro cells. Eryxin significantly down-regulated the mRNA and protein expression of aromatase protein by decreasing the expression of aromatase Promoter Ⅱ. Boiling and enzymolysis significantly attenuated the inhibitory effect of eryxin on estrogen biosynthesis in KGN cells. Eryxin suppressed estrogen biosynthesis in KGN cells by the inhibition of aromatase expression, and it warrants further investigation as a candidate for the treatment of estrogen-related diseases.

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更新日期/Last Update: 2019-10-25