|本期目录/Table of Contents|

[1]杨婧,栾云鹏,卢治宇,等.Trichoderma sp. WL-Go细胞提取物合成纳米金影响因素及其催化特性[J].应用与环境生物学报,2019,25(06):1451-1456.[doi:10.19675/j.cnki.1006-687x.2019.01021]
 YANG Jing,LUAN Yunpeng,LU Zhiyu,et al.Influencing factors and catalytic characteristics of gold nanoparticles biosynthesized with the cell-free extracts of Trichoderma sp. WL-Go[J].Chinese Journal of Applied & Environmental Biology,2019,25(06):1451-1456.[doi:10.19675/j.cnki.1006-687x.2019.01021]
点击复制

Trichoderma sp. WL-Go细胞提取物合成纳米金影响因素及其催化特性
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年06期
页码:
1451-1456
栏目:
研究论文
出版日期:
2019-12-30

文章信息/Info

Title:
Influencing factors and catalytic characteristics of gold nanoparticles biosynthesized with the cell-free extracts of Trichoderma sp. WL-Go
作者:
杨婧栾云鹏卢治宇白健冬章洪彬李炫莹马桥曲媛媛
1大连理工大学环境学院,工业生态与环境工程教育部重点实验室 大连 116024 2大连海事大学环境科学与工程学院,环境系统生物学研究所 大连 116026
Author(s):
YANG Jing1 LUAN Yunpeng1 LU Zhiyu1 BAI Jiandong1 ZHANG Hongbin1 LI Xuanying1 MA Qiao2 & QU Yuanyuan1**
1 Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China 2 BInstitute of Environmental Systems Biology, College of Environmental Science and Engineering, Dalian Maritime University, Dalian 116026, China
关键词:
纳米金生物合成Trichoderma sp.4-硝基苯酚催化活性
Keywords:
gold nanoparticle biosynthesis Trichoderma sp. 4-nitrophenol catalytic activity
分类号:
X172
DOI:
10.19675/j.cnki.1006-687x.2019.01021
摘要:
与传统的物理和化学合成方法相比而言,生物法合成纳米金具备环境友好、反应条件温和、低毒等优势,近年来受到了广泛关注. 利用真菌Trichoderma sp. WL-Go细胞提取物合成纳米金,探究氯金酸浓度、pH、温度等反应条件对纳米金合成的影响,最后对生物纳米金催化还原4-硝基苯酚的性能进行了考察. 结果显示,氯金酸浓度、pH以及反应温度对纳米金的合成具有重要影响,影响主要体现在合成速率以及纳米金稳定性方面. 温度的提高可加速纳米金合成,通过TEM分析可知30 ℃和60 ℃条件下合成的纳米金平均粒径分别为15.0 nm和15.1 nm,均为球形和伪球形. 对4-硝基苯酚的催化还原试验表明,在60 ℃条件下合成的纳米金催化速率优于30 ℃条件下合成的纳米金. 本研究表明利用菌株Trichoderma sp. WL-Go细胞提取物可以合成尺寸均一且分散性良好的纳米金颗粒,且温度的提高可在加速纳米金合成的同时提升其催化活性,对生物合成纳米金的工业化应用有一定积极意义. (图4 表1 参23)
Abstract:
Compared to physical or chemical synthesis methods, the biosynthesis of gold nanoparticles (AuNPs) has recently attracted extensive attention owing to its higher environmentally friendly nature, milder reaction conditions, and lower toxicity effects. In this study, cell-free extracts of Trichoderma sp. WL-Go were used to synthesize AuNPs. The effects of chloroauric acid concentration, pH, and temperature on the biosynthesis process were investigated, and the catalytic ability of as-synthesized AuNPs to reduce 4-nitrophenol was discussed. Based on our results, chlorometallic acid concentration, pH, and temperature had important effects on the synthesis of AuNPs, which were mainly reflected in the synthesis rate and stability of the AuNPs. An increase in temperature could accelerate the synthesis process and through TEM, the average particle size of the AuNPs synthesized at 30 ℃ and 60 ℃ was found to be 15.0 nm and 15.1 nm, respectively, with both spherical and pseudo-spherical appearances. According to the catalytic experimental results of the 4-nitrophenol reduction, the catalytic rate was higher for AuNPs synthesized at 60 ℃ than at 30 ℃. In conclusion, this study shows that the cell-free extracts of Trichoderma sp. WL-Go could be used to synthesize AuNPs with good dispersion and uniform size. Furthermore, an increase in temperature can accelerate the synthesis of AuNPs and enhance their catalytic activity, which have a positive significance in the industrial application of biosynthesized AuNPs.

参考文献/References:

1. Ayati A, Ahmadpour A, Bamoharram FF, Tanhaei B, M?ntt?ri M, Sillanp?? M. A review on catalytic applications of Au/TiO2 nanoparticles in the removal of water pollutant [J]. Chemosphere, 2014, 107: 163-174
2. Daniel MC, Astruc D. Gold nanoparticles: assembly, supramolecular chemistry, quantum-size-related properties, and applications toward biology, catalysis, and nanotechnology [J]. Chem Rev, 2004, 104 (1): 293-346
3. Sharma M, Pathak M, Roy B, Chand A, Dhanda G, Abbasi N, Panchal G. Green synthesis of gold nanoparticles and their characterization [J]. J Mat Nanosci, 2016, 3 (1): 8-10
4. Tabrizi A, Ayhan F, Ayhan H. Gold nanoparticle synthesis and characterization [J]. Hacet J Biol Chem, 2009, 37 (3): 217-226
5. Yazid H, Adnan R, Hamid SA, Farrukh MA. Synthesis and characterization of gold nanoparticles supported on zinc oxide via the deposition-precipitation method [J]. Turk J Chem, 2010, 34: 639-650
6. Cioffi N, Colaianni L, Ieva E, Pilolli R, Ditaranto N, Angione MA, Cotrone S, Buchholt K, Spetz AL, Sabbatini L, Torsi L. Electrosynthesis and characterization of gold nanoparticles for electronic capacitance sensing of pollutants [J]. Electrochim Acta, 2011, 56 (10): 3713-3720
7. Mccaffrey R, Long H, Jin Y, Sanders A, Park W, Zhang W. Template synthesis of gold nanoparticles with an organic molecular cage [J]. JACS, 2014, 136 (5): 1782-1785
8. Ahmad T, Irfan M, Bustam MA, Bhattacharjee S. Effect of reaction time on green synthesis of gold nanoparticles by using aqueous extract of Elaise guineensis (oil palm leaves) [J]. Procedia Eng, 2016, 148: 467-472
9. 刘紫嫣, 周豪, 沈娥, 王经伟, 张照婧, 沈文丽, 马桥, 曲媛媛, 周集体. 微生物介导的金纳米颗粒合成[J]. 微生物学通报, 2015, 42 (8): 1584-1592 [Liu ZY, Zhou H, Shen E, Wang JW, Zhang ZJ, Shen WL, Ma Q, Qu YY, Zhou JT. Recent advances on microbial mediated synthesis of gold nanoparticles [J]. Microbiol Chin, 2015, 42 (8): 1584-1592]
10. 厉舒祯, 沈文丽, 刘洋荧, 邓晔, 由胜男, 裴晓芳, 李会杰, 张照婧, 曲媛媛. 真菌介导的纳米金合成及其应用研究进展[J]. 环境科学与技术, 2016, 39 (9): 82-87 [Li SZ, Shen WL, Liu YY, Deng Y, You SN, Pei XF, Li HJ, Zhang ZJ, Qu YY. Research advances in fungi-mediated biosynthesis of gold nano-particle and its application [J]. Environ Sci Technol, 2016, 39 (9): 82-87]
11. Harman GE. Trichoderma-not just for biocontrol anymore [J]. Phytoparasitica, 2011, 39: 103-108
12. Qu YY, Shen WL, Pei XF, Ma F, You SN, Li SZ, Zhou JT. Biosynthesis of gold nanoparticles by Trichoderma sp. WL-Go for azo dyes decolorization [J]. J Environ Sci, 2017, 6: 79-86
13. Fazekas SSG, Webster RG, Datyner A. Two new staining procedures for quantitative estimation of proteins on electrophoretic strips [J]. Biochim Biophys Acta, 1963, 71: 377-391
14. Shankar SS, Ahmad A, Pasricha R, Sastry M. Bioreduction of chloroaurate ions by geranium leaves and its endophytic fungus yields gold nanoparticles of different shapes [J]. J Mater Chem, 2003, 13 (7): 1822-1826
15. Yang CX, Sun XY, Liu B. Controllable synthesis of chitosan-mediated gold nanoplate and the growth mechanisms [J]. Acta Chim Sin, 2012, 70 (3): 259-264
16. Wu XQ, Wu XW, Huang Q, Shen JS, Zhang HW. In situ synthesized gold nanoparticles in hydrogels for catalytic reduction of nitroaromatic compounds [J]. Appl Surf Sci, 2015, 331: 210-218
17. Panigrahi S, Basu S, Praharaj S, Pande S, Jana S, Pal A, Ghosh SK, Pal T. Synthesis and size-selective catalysis by supported gold nanoparticles: study on heterogeneous and homogeneous catalytic process [J]. J Phys Chem C, 2007, 111 (12): 4596-4605
18. Shen WL, Qu YY, Pei XF, Li SZ, You SN, Wang JW, Zhang ZJ, Zhou JT. Catalytic reduction of 4-nitrophenol using gold nanoparticles biosynthesized by cell-free extracts of Aspergillus sp. WL-Au [J]. J Hazard Mater, 2017, 321: 299-306
19. Brodersen SH, Gr?nbjerg U, Hvolb?k B. Understanding the catalytic activity of gold nanoparticles through multi-scale simulations [J]. J Catal, 2011, 284 (1): 34-41
20. 李炫莹, 沈文丽, 张旭旺, 裴晓芳, 李亚飞, 殷庆鑫, 由胜男, 王经伟, 曲媛媛. Trichosporon montevideense WIN合成纳米金的催化特性[J]. 微生物学通报, 2017, 44 (8): 1858-1865 [Li XY, Shen WL, Zhang XW, Pei XF, Li YF, Yin QX, You SN, Wang JW, Qu YY. Catalytic characteristics of gold nanoparticles biosynthesized by Trichosporon montevideense WIN [J]. Microbiol Chin, 2017, 44 (8): 1858-1865]
21. Das SK, Dickinson C, Lafir F, Brougham DF, Marsili E. Synthesis, characterization and catalytic activity of gold nanoparticles biosynthesized with Rhizopus oryzae protein extract [J]. Green Chem, 2012, 14: 1322-1334
22. Das SK, Liang J, Schmidt M, Laffir F, Marsili E. Biomineralization mechanism of gold by zygomycete fungi Rhizopous oryzae [J]. Acs Nano, 2012, 6 (7): 6165-6173
23. Mishra A, Kumari M, Pandey S, Chaudhury V, Gupta KC, Nautiya CS. Biocatalytic and antimicrobial activities of gold nanoparticles synthesized by Trichoderma sp. [J]. Bioresource Technol, 2014, 166: 235-242
24.

相似文献/References:

[1]马桥,曲媛媛,张旭旺,等.靛蓝的微生物合成研究新进展[J].应用与环境生物学报,2012,18(02):344.[doi:10.3724/SP.J.1145.2012.00344]
 MA Qiao,QU Yuanyuan**,ZHANG Xuwang,et al.Recent Advances in Microbial Synthesis of Indigo[J].Chinese Journal of Applied & Environmental Biology,2012,18(06):344.[doi:10.3724/SP.J.1145.2012.00344]
[2]许炳雯,李诗阳,张强,等.细菌芳烃外二醇双加氧酶研究进展[J].应用与环境生物学报,2012,18(05):873.[doi:10.3724/SP.J.1145.2012.00873]
 XU Bingwen,LI Shiyang,ZHANG Qiang,et al.Advance in Research on Bacterial Aromatic Extradiol Dioxygenase[J].Chinese Journal of Applied & Environmental Biology,2012,18(06):873.[doi:10.3724/SP.J.1145.2012.00873]
[3]苏敏,蒋永,张尧,等.生物电化学耦合H2还原CO2合成简单有机物[J].应用与环境生物学报,2013,19(05):827.[doi:10.3724/SP.J.1145.2013.00827]
 SU Min,JIANG Yong,ZHANG Yao,et al.Coupled Bioelectrochemical System for Reducing CO2 to Simple Organic Compounds in the Presence of H2[J].Chinese Journal of Applied & Environmental Biology,2013,19(06):827.[doi:10.3724/SP.J.1145.2013.00827]
[4]李筱筱,刘炜,咸漠,等.大肠杆菌乙酰酯酶(AES)的酶学性质[J].应用与环境生物学报,2017,23(06):1011.[doi:10.3724/SP.J.1145.2016.12009]
 LI Xiaoxiao,LIU Wei,XIAN Mo & WANG Wei**.Characterization of Escherichia coli acetyl esterase (AES) activity[J].Chinese Journal of Applied & Environmental Biology,2017,23(06):1011.[doi:10.3724/SP.J.1145.2016.12009]
[5]裴晓芳,沈文丽,由胜男,等.Trichosporon montevideense WIN提取物合成纳米金及其对硝基芳烃的催化特性[J].应用与环境生物学报,2017,23(03):409.[doi:2016.06004]
 PEI Xiaofang,SHEN Wenli,YOU Shengnan,et al.Biosynthesis of gold nanoparticles by the cell-free extracts of Trichosporon?montevideense WIN for catalytic reduction of nitroaromatics[J].Chinese Journal of Applied & Environmental Biology,2017,23(06):409.[doi:2016.06004]
[6]张珩琳,杨婧,周浩,等.Cupriavidus metallidurans SHE胞内提取物合成纳米金及其催化应用[J].应用与环境生物学报,2019,25(02):457.[doi:10.19675/j.cnki.1006-687x.2018.07023]
 ZHANG Henglin,YANG Jing,ZHOU Hao,et al.Biosynthesis of gold nanoparticles by the cell-free extracts of Cupriavidus metallidurans SHE and its catalytic applications[J].Chinese Journal of Applied & Environmental Biology,2019,25(06):457.[doi:10.19675/j.cnki.1006-687x.2018.07023]

更新日期/Last Update: 2019-12-25