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[1]高卓田,牛小云,周健,等.种植杞柳在镉污染土壤修复过程中对土壤微生物群落结构和理化性质的影响[J].应用与环境生物学报,2019,25(03):501-509.[doi:10.19675/j.cnki.1006-687x.201901016]
 GAO Zhuotian,NIU Xiaoyun,ZHOU Jian,et al.Effects of planting Sailx integra on the microbial community structure and the physical and chemical properties during cadmium contaminated soil remediation[J].Chinese Journal of Applied & Environmental Biology,2019,25(03):501-509.[doi:10.19675/j.cnki.1006-687x.201901016]
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种植杞柳在镉污染土壤修复过程中对土壤微生物群落结构和理化性质的影响()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年03期
页码:
501-509
栏目:
重金属污染及生物修复专栏
出版日期:
2019-06-25

文章信息/Info

Title:
Effects of planting Sailx integra on the microbial community structure and the physical and chemical properties during cadmium contaminated soil remediation
作者:
高卓田牛小云周健王芸黄大庄
高卓田1 牛小云1 周 健1 王 芸2 黄大庄1**
Author(s):
GAO Zhuotian1 NIU Xiaoyun1 ZHOU Jian1 WANG Yun2 & HUANG Dazhuang1**
1 College of Landscape and Travel, Agricultural?University?of Hebei, Baoding 071000, China 2 Shijiazhuang Botanical Garden Management Office, Shijiazhuang 050000, China
关键词:
杞柳镉污染养分微生物代谢活性微生物群落结构
Keywords:
Salix integra cadmium pollution nutrient microbial metabolic activity microbial community structure
分类号:
Q948.122.3
DOI:
10.19675/j.cnki.1006-687x.201901016
摘要:
研究种植杞柳在镉污染土壤修复过程中对微生物群落结构的影响,可为应用杞柳-微生物联合修复镉污染土壤提供理论依据. 以 1年生杞柳扦插苗为试验材料进行盆栽试验,设置4个镉浓度梯度处理(0、50、100以及150 mg/kg),每个处理同时设置种植杞柳组和未种植杞柳组. 种植杞柳1年后收集根际、非根际以及未种植杞柳土壤,对土壤理化性质、不同形态重金属含量进行测定,利用Biolog-ECO平板法对微生物代谢功能多样性进行测定以及利用T-RFLP法对微生物群落结构多样性进行测定. 采用冗余分析(RDA)对微生物群落结构多样性与土壤理化性质、不同形态重金属含量的相关性进行分析. 结果表明,在镉污染处理组中养分含量总体呈现种植杞柳组高于未种植杞柳组,不同土壤来源对6类碳源的平均利用率存在显著差异,碳水化合物和酚酸类相对利用率呈现种植杞柳土壤<未种植土壤,而氨基酸、羧酸、多聚物以及胺类相对利用率都在根际土壤中最高. 对照组与处理组根际微生物丰富度与多样性指数差异不显著,但优势微生物种类明显不同. RDA分析表明,与对照相比,受污染的土壤中微生物与环境因子的相关性更强,可还原态镉与优势菌株B-T-RFs(137)、B-T-RFs(141)等有较强的相关性. 综上,种植杞柳能显著促进镉污染土壤中微生物代谢活性、微生物丰富度指数以及多样性指数,对优势微生物种群、代谢组成有明显影响;影响根际与非根际土壤中优势微生物的主要环境因子不同,污染组微生物受环境影响更大. (图8 表1 参50)
Abstract:
In order to study the effects of planting Salix integra on the microbial community structure in cadmium-contaminated soil, a pot experiment was carried out with one-year old cuttings of seedlings as starting material. We set up un-planted and planted S. integra areas with four levels of cadmium treatments (0, 50, 100, and 150 mg/kg). After one year of planting, the rhizosphere soil, non-rhizosphere soil, and unplanted soil were collected. The physical and chemical properties of the soil and the fractionation of heavy metal content were determined. The Biolog-ECO plate method was used to measure the microbial metabolic functional diversity and the T-RFLP method was used to measure the diversity of the microbial community structure. Redundancy analysis (RDA) was used to analyze the correlation between microbial community structure diversity, physical and chemical properties of the soil, and fractionation of heavy metal content. The nutrient content in the cadmium-contaminated treatment group was higher than that in the un-planted group. The average utilization rate of the six types of carbon sources was significantly different among different soil sources. The relative utilization ratio of carbohydrates and phenolic acids was higher in un-planted soil than in planted soil, and the amount of amino acids, carboxylic acids, polymers, and amines was the highest in rhizosphere soil. There was no significant difference in the rhizosphere microbial richness and the diversity index between the control group and the treatment group, but the dominant microbial species were significantly different. RDA analysis showed that the microorganisms in the contaminated soil were more strongly correlated with environmental factors, and the reducible cadmium had a strong correlation with the dominant B-T-RFs (137), and B-T-RFs (141). Planting S. integra can significantly promote microbial metabolic activity, microbial richness index, and diversity index in cadmium-contaminated soil, which in turn has a significant impact on the dominant microbial population and metabolic composition. The main environmental factors affecting dominant microorganisms in rhizosphere and non-rhizosphere soils are different. Microorganisms in the treatment group are more affected by the environment.

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更新日期/Last Update: 2019-06-25