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[1]孙志鹏,蒲擎宇,尚鹏程,等.云南红豆杉活性成分10-DAB累积分布规律[J].应用与环境生物学报,2019,25(05):1161-1167.[doi:10.19675/j.cnki.1006-687x.2019.01006]
 SUN Zhipeng,Pu Qingyu,SHANG Pengcheng,et al.Cumulative distribution regularities of the active component 10-DAB of Taxus yunnanensis[J].Chinese Journal of Applied & Environmental Biology,2019,25(05):1161-1167.[doi:10.19675/j.cnki.1006-687x.2019.01006]
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云南红豆杉活性成分10-DAB累积分布规律
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年05期
页码:
1161-1167
栏目:
研究论文
出版日期:
2019-10-31

文章信息/Info

Title:
Cumulative distribution regularities of the active component 10-DAB of Taxus yunnanensis
作者:
孙志鹏蒲擎宇尚鹏程曾小珂詹秀文王刚
四川农业大学林学院 成都 611830
Author(s):
SUN Zhipeng Pu Qingyu SHANG Pengcheng ZENG XiaokeZHAN Xiuwen & WANG Gang**
College of Forestry, Sichuan Agricultural University, Chengdu, 611830, China
关键词:
云南红豆杉HPLC10-DAB累积生长时期海拔
Keywords:
Taxus yunnanensis HPLC 10-DAB accumulation growth stage altitude
分类号:
Q949.660.6
DOI:
10.19675/j.cnki.1006-687x.2019.01006
摘要:
为研究云南红豆杉10-DAB的累积规律及单株10-DAB累积量,采用HPLC法测定不同海拔、不同生长时期、不同采收期的云南红豆杉枝叶中10-DAB含量,同时测定枝叶生物量. 结果显示:红豆杉中10-DAB累积受季节影响显著,从4月份到10月份呈现逐渐递增趋势,10月份10-DAB累积达到最高,之后呈下降趋势;生长年限为2年时,其10-DAB累积最高,且与3、4、5年相比具有显著差异(P < 0.05),之后随生长年限增加10-DAB累积逐年下降;10-DAB累积在海拔(900 ± 50)m以下无显著性差异(P > 0.05),高于(900 ± 50)m后随海拔增加而降低,且与(500 ± 50)m相比有极显著性差异(P < 0.01);生长年限相较于海拔,权重占比更大,对10-DAB的累积影响更大;从单株10-DAB累积量来看,海拔高于900 m,单株枝叶10-DAB累积量随海拔升高而呈下降趋势;3年生单株枝叶10-DAB累积量大于2、4和5年生,单株10-DAB累积量最高可达0.697 ± 0.026 g. 本研究表明,生长年限、季节、海拔、生物量均显著影响云南红豆杉单株10-DAB累积量,适宜种植云南红豆杉10-DAB药用原料林的最佳海拔范围应在500-900 m,最佳采收生长年限为第3-4年,采收季节10月份;结果可为云南红豆杉最佳生长条件的选择及科学采收提供理论依据. (图6 表2 参33)
Abstract:
We used high performance liquid chromatography to measure the accumulation of 10-Deacetylbaccatin (10-DAB) in the branches and leaves of Taxus yunnanensis at different altitudes, different growth stages, and different harvest periods. We also measured the biomass of leaves and branches. The accumulation of 10-DAB in T. yunnanensis was significantly influenced by season: from April to October, it showed a gradually increasing trend, and reached a maximum in October, and then showed a decreasing trend. The rate of 10-DAB accumulation was highest in two-year-old plants; after this, the rate of 10-DAB accumulation showed a decreasing trend. The total accumulated amount of 10-DAB reached a maximum in the branches and leaves of three-year-old single plants. The highest total accumulated amount of 10-DAB from a single plant was 0.697 ± 0.026 g. There was no significant difference in the rate of accumulation of 10-DAB in samples collected from below 900 ± 50 m altitude. In samples collected from above 900 ± 50 m altitude, the rate of 10-DAB accumulation decreased with increasing altitude; moreover, there was a very significant difference in samples collected at 900 ± 50 m altitude when compared with samples collected at 500 ± 50 m altitude. Compared with altitude, age was a more important factor influencing rate of 10-DAB accumulation. Age, season, altitude, and biomass all significantly influenced accumulation of 10-DAB in single plants of T. yunnanensis. From the point of view of 10-DAB production for medicinal purposes, the optimum altitude range for T. yunnanensis plantation is 500-900 m, the optimum harvest age is three to five years old, and the optimum harvesting season is October.

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更新日期/Last Update: 2019-10-25