|本期目录/Table of Contents|

[1]陈青,王红妹,李晓红,等.氯乙酰胺类除草剂微生物降解研究进展[J].应用与环境生物学报,2019,25(05):1252-1260.[doi:10.19675/j.cnki.1006-687x.2018.12056]
 CHEN Qing,WANG Hongmei**,et al.Research progress in microbial degradation of chloroacetanilide herbicides[J].Chinese Journal of Applied & Environmental Biology,2019,25(05):1252-1260.[doi:10.19675/j.cnki.1006-687x.2018.12056]
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氯乙酰胺类除草剂微生物降解研究进展
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年05期
页码:
1252-1260
栏目:
综述
出版日期:
2019-10-31

文章信息/Info

Title:
Research progress in microbial degradation of chloroacetanilide herbicides
作者:
陈青王红妹李晓红尉鸿飞苏朋何健蒋建东
1枣庄学院生命科学学院 枣庄 277100 2南京农业大学农业部农业环境微生物生物工程重点实验室 南京 210095 3南京农业大学资源与环境科学学院 南京 210095
Author(s):
CHEN Qing1 3 WANG Hongmei1** LI Xiaohong1 WEI Hongfei1 SU Peng1 HE Jian2** & JIANG Jiandong2
1 College of Life Sciences, Zaozhuang University, Zaozhuang 277100, China 2 Key Laboratory of Microbiological Engineering Agricultural Environment, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China 3 College of Environmental and Resource Sciences, Nanjing Agricultural University, Nanjing 210095, China
关键词:
氯乙酰胺类除草剂微生物降解生物修复
Keywords:
chloroacetanilide herbicide microbial degradation bioremediation
分类号:
X172
DOI:
10.19675/j.cnki.1006-687x.2018.12056
摘要:
氯乙酰胺类除草剂主要分子结构由苯环和氯乙酰胺基团组成,在世界范围内的产量和使用量仅次于草甘膦和磺酰脲类除草剂. 其大量使用导致在环境中残留量不断增加,已经对生态环境和人类健康造成了严重威胁. 该类除草剂在环境中的消解主要是通过微生物代谢实现的,本文从微生物降解菌株资源、降解途径、关键基因和酶等几个方面综述了国内外微生物降解该类除草剂的最新研究进展. 细菌主要采用厌氧谷胱甘肽-共轭脱氯和好氧脱烷基-苯环开环代谢途径降解该类除草剂;真菌降解该类除草剂的途径多样化且比较复杂,尚需进一步研究. 从细菌中克隆出N-脱烷基酶、酰胺酶和羟化酶基因,但是对于上述酶学特性研究不够深入. 同时指出,目前有关微生物降解该类除草剂的研究主要集中于甲草胺、乙草胺和丁草胺,对于异丙草胺和异丙甲草胺微生物降解的代谢途径和分子机制方面的研究仍较少,在今后的工作中要加强异丙草胺和异丙甲草胺微生物降解方面的研究. 此外,微生物降解该类除草剂的新型基因为抗除草剂作物新品种的构建提供了良好的基因资源. (图6 表1 参69)
Abstract:
Chloroacetanilide herbicides are a class of highly efficient preemergence herbicides that are widely used across the world. Presently, the production and use of the chloroacetanilide herbicides is less than glyphosate and sulfonylurea herbicides. Due to their widespread use, long persistence, and high-water solubility, some of these herbicides have frequently been detected in the environment and have shown toxic effects on humans. Microbial metabolism is the most important factor in the degradation of chloroacetanilide herbicides in the environment. In this paper, we summarized the latest research progress on the microbial strain isolation, identification, biodegradation pathway, and molecular mechanisms (such as key genes and enzymes) of these herbicides. The anaerobic-bacteria degradation of the pathway is glutathione-acetanilide conjugates which can be further converted to sulfonated metabolites. The aerobic-bacteria degradation of the pathway is mainly initiated by N/C-dealkylation events, followed by aromatic ring hydroxylation and cleavage processes. The fungal degradation pathways of chloroacetanilide herbicides are more complex, and therefore further research is needed. Though, the genes of N-dealkylase, amidase and hydroxylase have been cloned, the characteristics of these enzymes have not been thoroughly studied. Currently, the research has focused on biodegradation of alachlor, acetochlor and butachlor; however, relatively little is known about the metabolic pathways and molecular mechanisms for biodegradation of propisochlor and metolachlor. Therefore, we should strengthen the research on biodegradation of propisochlor and metolachlor. In particular, research is needed on the chiral selectivity of the microorganisms and enzymes to the isomers of metolachlor. In addition, new genes for microbial degradation of this herbicide may provide genetic resources for transgenic crops.

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更新日期/Last Update: 2019-10-25