|本期目录/Table of Contents|

[1]张姣惠,胡可慧,陈珍珍,等.亚抑菌浓度氟苯尼考作用下质粒pSD11对大肠杆菌适应性的影响[J].应用与环境生物学报,2019,25(05):1211-1214.[doi:10.19675/j.cnki.1006-687x.2018.12045]
 ZHANG Jiaohui,HU Kehui,CHEN Zhenzhen,et al.Effect of plasmid pSD11 on the fitness of Escherichia coli in sub-minimum inhibitory concentration of florfenicol[J].Chinese Journal of Applied & Environmental Biology,2019,25(05):1211-1214.[doi:10.19675/j.cnki.1006-687x.2018.12045]
点击复制

亚抑菌浓度氟苯尼考作用下质粒pSD11对大肠杆菌适应性的影响
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年05期
页码:
1211-1214
栏目:
研究论文
出版日期:
2019-10-31

文章信息/Info

Title:
Effect of plasmid pSD11 on the fitness of Escherichia coli in sub-minimum inhibitory concentration of florfenicol
作者:
张姣惠胡可慧陈珍珍邓辉黄小红
1福建农林大学动物科学学院 福州 350002 2中西兽医结合与动物保健福建省高等学校重点实验室 福州 350002
Author(s):
ZHANG Jiaohui12 HU Kehui12 CHEN Zhenzhen12DENG Hui12** & HUANG Xiaohong12**
1Fujian Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health, Fujian Agriculture And Forestry University, Fuzhou 350002, China 2University Key Laboratory for Integrated Chinese Traditional and Western Veterinary Medicine and Animal Healthcare in Fujian Province, Fuzhou 350002, China
关键词:
质粒pSD11cfr大肠杆菌氟苯尼考适应性最低筛选浓度(MSC)
Keywords:
plasmid pSD11 cfr Escherichia coli florfenicol fitness the minimum selectable concentrations (MSC)
分类号:
Q939.97 : Q786
DOI:
10.19675/j.cnki.1006-687x.2018.12045
摘要:
通过分析cfr阳性质粒pSD11对大肠杆菌的适应性影响, 并测定氟苯尼考对携带质粒pSD11的大肠杆菌MG1655(Escherichia coli MG1655)的最低筛选浓度(MSC),评价亚抑菌浓度氟苯尼考作用下多重耐药基因cfr在大肠杆菌中的持续存在和扩散传播的风险. 通过电转化的方法将质粒pSD11导入模式菌E. coli MG1655中,获得耐药菌株E. coli MG1655/pSD11. 通过测定生长曲线,比较无抗生素作用下耐药菌株E. coli MG1655/pSD11与同源敏感菌株E. coli MG1655的生长动力学参数,评价质粒pSD11对宿主菌适应性的影响. 并通过比较分析不同亚抑菌浓度(0.031 25,0.125,0.25,0.5,1.0和2.0 μg/mL)氟苯尼考作用下的生长速率,测定氟苯尼考对携带质粒pSD11的宿主菌最小筛选浓度(MSC). 携带质粒pSD11的耐药菌E. coli MG1655/pSD11的延滞时间(2.853 3 ± 0.031 8 h/λ)相对于同源敏感菌E. coli MG1655极显著延长(2.825 3 ± 0.024 4 h/λ),且世代时间也显著延长(分别为:1.705 7 ± 0.006 4 h/G和1.526 9 ± 0.007 8 h/G). 氟苯尼考对耐药菌E. coli MG1655/pSD11的MSC为0.042 5 μg/mL约为相应敏感菌的MICsus的1/100. 本研究表明携带cfr基因的质粒pSD11在无抗生素选择下对宿主菌有明显的适应性影响,但是在极低的氟苯尼考浓度的作用下存在选择优势,能够平衡适应性成本使宿主菌得到富集与传播,以期为对该质粒在环境中的传播风险评估及氟苯尼考的临床应用提供的参考. (图2 表2 参18)
Abstract:
The sustainability and spread of the multi-resistant gene cfr in Escherichia coli was evaluated by analyzing the fitness effect of the cfr-carrying plasmid pSD11 in E. coli, and determining the minimum selectable concentration (MSC) of florfenicol for pSD11-harboring E. coli. Plasmid pSD11 was transferred into E. coli MG1655 by electroporation to obtain the drug-resistant strain E. coli MG1655/pSD11. The growth kinetics parameters of this strain and the homologous sensitive strain E. coli MG1655 were compared by measuring the growth curve without antibiotics. The MSC for strain E. coli MG1655/pSD11 was determined by analyzing and comparing the growth rates of strain E. coli MG1655/pSD11 and E. coli MG1655 at different sub-minimum inhibitory concentrations (0.031 25, 0.062 5, 0.125, 0.25, 0.5, 1.0, and 2.0 μg/mL) of florfenicol. The lag time of the drug-resistant with plasmid pSD11 strain E. coli MG1655/pSD11 (2.853 3 ± 0.031 8 h/λ) is significantly longer than that the homologous sensitive E. coli MG1655 (2.825 3 ± 0.024 4 h/λ), and the generation time was also significantly longer ± 0.006 4 h/G and 1.526 9 ± 0.007 8 h/G, respectively). The MSC of florfenicol for strain E. coli MG1655/pSD11 was 0.042 5 μg/mL, which was 1/100 of the MICsus value of the homologous sensitive strain E. coli MG1655. The cfr-carrying plasmid pSD11 has a significant adaptive effect on the host bacteria. However, it can also possess the host strain of selective advantage under extremely low concentration of florfenicol, which can balance the fitness cost of plasmid and benefit the enrichment and transmission of host bacteria.

参考文献/References:

1 Schwarz S, Werckenthin C, Kehrenberg C. Identification of a plasmid-borne chloramphenicol-florfenicol resistance gene in Staphylococcus sciuri [J]. Antimicrob Agents Chemother, 2000, 44 (9): 2530-2533
2 Vester B. The cfr and cfr-like multiple resistance genes [J]. Res Microbiol, 2018, 169 (2): 61-66
3 Sun J, Deng H, Li L, Chen MY, Fang LX, Yang QE, Liu YH, Liao XP. Complete nucleotide sequence of cfr-carrying IncX4 plasmid pSD11 from Escherichia coli [J]. Antimicrob Agents Chemother, 2015, 59 (1): 738-741
4 Wang X, Zhu Y, Hua X, Chen F, Wang C, Zhang Y, Liu S, Zhang W. F14:A-:B- and incx4 inc group cfr-positive plasmids circulating in escherichia coli of animal origin in northeast china [J]. Veterinary Microbiol, 2018, 217 (1): 53-57
5 Gullberg E, Albrecht LM, Karlsson C, Sandegren L, Andersson DI. Selection of a multidrug resistance plasmid by sublethal levels of antibiotics and heavy metals [J]. MBio, 2014, 5 (5): 1918-1914
6 Diarmaid H. Selection and evolution of resistance to antimicrobial drugs [J]. Iubmb Life, 2015, 66 (8): 521-529
7 Zhao Q, Wang Y, Wang S, Wang Z, Du X, Jiang H, Xia X, Shen Z, Ding S, Wu C, Zhou B, Wu Y, Shen J. Prevalence and abundance of florfenicol and linezolid resistance genes in soils adjacent to swine feedlots [J]. Sci Rep, 2016, 6: 32192-32199
8 Peng Z, Shen Z, Zhang C, Li S, Bing W, Shang J, Yue X, Qu Z, Li X, Wu L. Surveillance of antimicrobial resistance among Escherichia coli from chicken and swine, china, 2008-2015 [J]. Veterinary Microbiol, 2017, 203 (1): 49-55
9 朱森康, 黄磊, 李燕飞, 钟卫鸿, 徐志南. 制备高效大肠杆菌电转化感受态细胞和电转化条件的研究[J]. 生物技术通报, 2011, 2011 (10): 206-209 [Zhu SK, Huang L, Li YF, Zhong WH, Xu ZN. Preparation of efficient electrotransformation competent cells of Escherichia coli and condition of electrotransformation [J]. Biotechnol bull, 2011, 2011 (10): 206-209]
10 Liu H, Nan C, Feng C, Shuang T, Rui L. Impact of electro-stimulation on denitrifying bacterial growth and analysis of bacterial growth kinetics using a modified gompertz model in a bio-electrochemical denitrification reactor [J]. Bioresource Technol, 2017, 232 (1): 344-353
11 San Millan A, MacLean RC. Fitness costs of plasmids: a limit to plasmid transmission [J]. Microbiol Spectr, 2017, 5 (5): 1-12
12 Andersson DI, Hughes D. Antibiotic resistance and its cost: Is it possible to reverse resistance? [J]. Nat Rev Microbiol, 2010, 8 (4): 260-271
13 Deng H, Sun J, Ma J, Li L, Fang LX, Zhang Q, Liu YH, Liao XP. Identification of the multi-resistance gene cfr in escherichia coli isolates of animal origin [J]. PLoS One, 2014, 9 (7): e102378
14 LaMarre JM, Locke JB, Shaw KJ, Mankin AS. Low fitness cost of the multidrug resistance gene cfr [J]. Antimicrob Agents Chemother, 2011, 55 (8): 3714-3719
15 Khan S, Beattie TK, Knapp CW. The use of minimum selectable concentrations (MSC) for determining the selection of antimicrobial resistant bacteria [J]. Ecotoxicology, 2017, 26 (2): 283-292
16 Lindsey HA, Gallie J, Taylor S, Kerr B. Evolutionary rescue from extinction is contingent on a lower rate of environmental change [J]. Nature, 2013, 494 (7438): 463-467
17 Stevenson C, Hall JPJ, Brockhurst MA, Harrison E. Plasmid stability is enhanced by higher-frequency pulses of positive selection [J]. Proc Biol Sci, 2018, 285 (1870): 1-8
18 Zhang QQ, Ying GG, Pan CG, Liu YS, Zhao JL. Comprehensive evaluation of antibiotics emission and fate in the river basins of china: source analysis, multimedia modeling, and linkage to bacterial resistance [J]. Environ Sci Technol, 2015, 49 (11): 6772-6782

更新日期/Last Update: 2019-10-25