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[1]刘生财,潘君飞,王晓,等.MeJA对苋菜悬浮细胞类黄酮和类胡萝卜素累积及其代谢相关基因表达的影响[J].应用与环境生物学报,2019,25(05):1168-1175.[doi:10.19675/j.cnki.1006-687x.2018.12039]
 LIU Shengcai#,PAN Junfei#,WANG Xiao,et al.Effects of methyl jasmonate on the contents and related metabolic genes of flavonoids and carotenoids in suspension cells of Amaranthus tricolor L.[J].Chinese Journal of Applied & Environmental Biology,2019,25(05):1168-1175.[doi:10.19675/j.cnki.1006-687x.2018.12039]
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MeJA对苋菜悬浮细胞类黄酮和类胡萝卜素累积及其代谢相关基因表达的影响
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年05期
页码:
1168-1175
栏目:
研究论文
出版日期:
2019-10-31

文章信息/Info

Title:
Effects of methyl jasmonate on the contents and related metabolic genes of flavonoids and carotenoids in suspension cells of Amaranthus tricolor L.
作者:
刘生财潘君飞王晓赵春丽赖钟雄张梓浩
福建农林大学园艺植物生物工程研究所 福州 350002
Author(s):
LIU Shengcai# PAN Junfei# WANG Xiao ZHAO Chunli LAI Zhongxiong ** & ZHANG Zihao
Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
关键词:
苋菜悬浮细胞MeJA类黄酮类胡萝卜素基因表达
Keywords:
Amaranthus tricolor L. suspension cells methyl jasmonate flavonoids carotenoids gene expression
分类号:
S636.4 : Q78
DOI:
10.19675/j.cnki.1006-687x.2018.12039
摘要:
茉莉酸甲酯(MeJA)对植物生长及次生代谢产物的合成具有重要的调控作用. 为了解MeJA对苋菜细胞中类黄酮和类胡萝卜素合成的影响,以苋菜悬浮细胞为材料,检测不同浓度MeJA和添加时间处理对类黄酮和类胡萝卜素累积的影响,结合实时荧光定量PCR技术,分析类黄酮和类胡萝卜素合成相关基因表达与其累积的关系. 结果显示,200 μmol/L MeJA处理下,苋菜悬浮细胞干重、类黄酮和类胡萝卜素产量均达到最大,在悬浮细胞培养的第4天添加200 μmol/L时类黄酮和类胡萝卜素的含量和产量达到最大;与对照(MeJA 0 μmol/L)相比,不同浓度MeJA处理下,PAL、F3H、CHI、CHS基因表达量均极显著上调,与类黄酮含量具有正相关性,PDS、PSY和ZDS基因也极显著上调表达,与类胡萝卜素含量具有正相关性;随着MeJA添加时间的延迟,PAL、F3H、CHS和CHI基因的表达模式与类黄酮含量无明显相关性,PDS和PSY表达模式基本一致,且与类胡萝卜素含量的变化趋势保持一致,ZDS基因则出现下调表达. 本研究表明MeJA浓度和添加时间对苋菜悬浮细胞中类黄酮和类胡萝卜合成起着重要调控作用,可为进一步探讨MeJA诱导苋菜悬浮细胞类黄酮和类胡萝卜素合成机制奠定基础. (图8 表1 参28)
Abstract:
Methyl jasmonate (MeJA) plays an important role in the regulation of plant growth and secondary metabolite synthesis. The effects of different concentrations of MeJA and time of addition on the content and yield of flavonoids and carotenoids were studied using suspension cells of amaranth (Amaranthus tricolor L.). The relationship between the expression of flavonoids and carotenoids-related genes and their accumulation was analyzed by real-time quantitative polymerase chain reaction (qPCR). The results showed that the dry weight, and flavonoid and carotenoid production of amaranth suspension cells reached the maximum at 200 μmol/L MeJA treatment. The content and yield of flavonoids and carotenoids reached the maximum when MeJA was added at 200 μmol/L on the fourth day of suspension cell culture. Under different concentrations of MeJA, the expression levels of PAL, F3H, CHI and CHS were significantly up-regulated, and were positively correlated with flavonoid content; PDS, PSY and ZDS genes were also significantly up-regulated and were positively correlated with carotene content. When MeJA was added later, the expression patterns of PAL, F3H, CHS and CHI genes were not significantly correlated with flavonoid content; PDS and PSY expression patterns were similar, and were consistent with the change of carotenoid content; and the ZDS gene was down-regulated. The concentration and addition time of MeJA played an important role in the regulation of flavonoids and carotenoids in suspension cells of amaranth. The study lays a foundation for further study on the mechanism of MeJA-induced flavonoids and carotenoids in amaranth suspension cells.

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更新日期/Last Update: 2019-10-25