|本期目录/Table of Contents|

[1]陆静,张赤红,吴瑜.四川大叶黄精组培快繁技术[J].应用与环境生物学报,2019,25(05):1222-1227.[doi:10.19675/j.cnki.1006-687x.2018.11043]
 LU Jing,ZHANG Chihong** & WU Yu**.Investigating tissue culture technique of a Polygonatum kingianum variety distributed in Sichuan[J].Chinese Journal of Applied & Environmental Biology,2019,25(05):1222-1227.[doi:10.19675/j.cnki.1006-687x.2018.11043]
点击复制

四川大叶黄精组培快繁技术
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年05期
页码:
1222-1227
栏目:
研究简报
出版日期:
2019-10-31

文章信息/Info

Title:
Investigating tissue culture technique of a Polygonatum kingianum variety distributed in Sichuan
作者:
陆静张赤红吴瑜
中国科学院成都生物研究所 成都 610041
Author(s):
LU Jing ZHANG Chihong** & WU Yu**
Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China
关键词:
大叶黄精根茎芽组织培养快繁体系
Keywords:
Polygonatum kingianum var. grandifolium root bud tissue culture micro propagation system
分类号:
Q813.12
DOI:
10.19675/j.cnki.1006-687x.2018.11043
摘要:
黄精市场需求量不断增加,较低的自然繁殖速度难以满足人们的需求. 通过组织培养技术,建立黄精快速繁殖技术体系,可以高效获得大量优质种苗在生产上应用. 首次以四川主载品种大叶黄精地下根茎芽为外植体进行组培快繁体系的初步研究. 结果显示:外植体消毒先用0.5 g/L的多菌灵溶液预处理12 h,再用0.2% HgCl2灭菌6 min + 6 min,污染率可得到有效控制;四川大叶黄精根茎芽最适宜的初代培养基为MS + 6-BA 0.5 mg/L + NAA 0.5 mg/L,萌发率达35%,长势健壮;不定芽分化培养基为MS + 6-BA 3.0 mg/L+ NAA 0.2 mg/L,平均不定芽诱导个数为4.33个;壮苗培养基为MS + 6-BA 1.5 mg/L + 2,4-D 0.5 mg/L + GA3 0.5 mg/L;生根培养基为1/2 MS + NAA 1.0 mg/L + IBA 0.2 mg/L + AC 0.2 g/L,生根率达89%. 本研究表明以大叶黄精的地下根茎芽为外植体可快速获得大量组培苗,其中细胞分裂素6-BA在大叶黄精生长过程中起着重要作用,结果可为四川提供便捷优质大叶黄精供苗途径. (表5 参26 附图4)
Abstract:
Current methods to propagate Polygonatum sibiricum are struggling to meet the rapidly increasing demand for Polygonatum. In order to provide high quality P. kingianum Coll. et Hemsl. var. grandifolium D. M. Liu et W. Z. Zeng, predominant cultivars of P. sibiricum in Sichuan, we developed a rapid micropropagation system using rhizome buds as explants. The results show that the contamination rate can be effectively controlled when the explants were pretreated with 0.5 g/L carbendazim solution for 12 h, before being sterilized with 0.2% HgCl2 for 6 min + 6 min. The optimal medium for primary culture was MS (Murashige and Skoog) + 6-BA 0.5 mg/L + NAA 0.5 mg/L; the induction rate for this medium was 35.0%. The optimal medium for adventitious bud induction was MS + 6-BA 3.0 mg/L + NAA 0.2 mg/L; the optimal medium for cultivating strong seeding was MS + 6-BA 1.5 mg/L + 2,4-D 0.5 mg/L + GA3 0.5 mg/L; the medium 1/2 MS + NAA 1 mg/L + IBA 0.2 mg/L + AC 0.2 g/L was suitable for the rooting and the rooting rate was 89%. A large number of tissue culture plantlets can be obtained quickly using the rhizome buds of P. sibiricum as explants. The cytokinin 6-BA plays an important role in the growth of P. sibiricum.

参考文献/References:

1 国家药典委员会. 中华人民共和国药典[M]. 北京: 中国医药科技出版社, 2015 [National Pharmacopoeia Commission. Chinese Pharmacopoeia [M]. Beijing: China Medical Science and Technology Press, 2015]
2 中国科学院中国植物志编辑委员会. 中国植物志[M]. 北京: 科学出版社. 2004 [Chinese Flora Editorial Board of the Chinese Academy of Sciences. Chinese Flora [M]. Beijing: Science Press. 2004]
3 四川植物志编委会. 四川植物志[M]. 成都: 四川人民出版社, 1981 [Sichuan Flora Editorial Board. Sichuan Flora [M]. Chengdu: Sichuan People’s Publishing House, 1981]
4 Luo CP, Li W, Chuna Z, Wu Y. Discriminating ?ve Polygonatum medical materials and monitoring their chemical changes associated with traditional process by FT-IR spectroscopy coupled with multivariate analysis [J]. Vibr Spectr, 2018, 99: 104-112
5 程强强, 罗嗣义, 刘相凤, 肖复明. 多花黄精不定芽诱导及植株再生[J]. 南方林业科学, 2018, 46 (6): 47-50 [Cheng QQ, Luo SY, Liu XF, Xiao FM. The adventitious buds induction and plant regeneration of Polygonatum cyrtonema [J]. S Chin For Sci, 2018, 46 (6): 47-50]
6 周新华, 厉月桥, 王丽云, 肖智勇, 钟文斌, 陈传松, 邓煌蔚, 黄维荣. 多花黄精根茎芽高效组培增殖和生根体系研究[J]. 经济林研究, 2016, 34 (1): 51-56 [Zhou XH, Li YQ, Wang LY, Xiao ZY, Zhong WB, Chen CS, Deng HW,Huang WR. An efficient proliferation and inducing roots system of tissue culture in Polygonatum cyrtonema [J]. Non-wood For Res, 2016, 34 (1): 51-56]
7 刘芳源, 王钰, 开桂青, 谢枫. 多花黄精组培体系建立及薯蓣皂苷等含量测定[J]. 生物学杂志, 2017, 34 (6): 93-96 [Liu FY, Wang Y, Kai GQ, Xie F. Establishment of tissue culture system and determination of yam saponins and diosgenin in Polygonatum sibiricum Hua [J]. J Biol, 2017, 34 (6): 93-96]
8 莫勇生, 卢拓方, 邱展鸿, 张红岩, 孙文波. 多花黄精组培苗快速繁殖体系建立研究[J]. 中国现代中药, 2018, 20 (4): 445-449 [Mo YS, Lu TF, Qiu ZH, Zhang HY, Sun WB. Study on the tissue culture and rapid propagation system of Polygonatum cyrtonema Hua [J]. Mod Chin Med, 2018, 20 (4): 445-449]
9 刘红美, 方小波, 夏开德, 杨苏文. 多花黄精组织培养快繁技术的研究[J]. 种子, 2010, 29 (12): 13-17 [Liu HM, Fang XB, Xia KD, Yang SW. Study on rapid micro propagation technology by tissue culture of P. cyrtonema Hua [J]. Seed, 2010, 29 (12): 13-17]
10 周建金, 罗晓锋, 叶炜, 江金兰. 多花黄精组培快繁技术研究[J]. 福建农业科技, 2012, 9: 59-61 [Zhou JJ, Luo XF, Ye W, Jiang JL. Tissue culture technique with rapid propagation for Polygonatum cyrtonema Hua [J]. Fujian Agric Sci Technol, 2012, 9: 59-61]
11 李莺, 王虹, 王晓臣, 赵兵, 杨婷. 鸡头黄精的组织培养与快速繁殖[J]. 陕西农业科学, 2011 (5): 104-108 [Li Y, Wang H, Wang XC, Zhao B, Yang T. Tissue culture and rapid micro propagation of Polygonatum sibircum Red. [J]. Shaanxi J Agric Sci, 2011 (5): 104-108]
12 黄登艳, 陈泽雄. 黄精根茎高效离体再生体系的建立[J]. 湖北农业科学, 2014, 53 (5): 1182-1184+1188 [Huang DY, Chen ZX. Establishing efficient regeneration system in vitro for rhizomes of Polygonatum sibiricum Delar. ex Redoute [J]. Hubei Agric Sci, 2014, 53 (5): 1182-1184+1188]
13 尹宏, 韩娇, 袁新普, 邹翠霞, 姜长阳. 黄精无性系建立的研究[J]. 西南农业学报, 2009, 22 (4): 1065-1068 [Yin H, Han J, Yuan XP, Zou CX, Jiang CY. Establishment of asexual line of Polygonaturm sibiricum Redoute [J]. Southwest Chin J Agric Sci, 2009, 22 (4): 1065-1068]
14 魏中华, 徐娟, 郭明霞, 石爱民, 孙继攻. 国内多菌灵的研究进展[J]. 安徽农业科学, 2015, 43 (3): 125-127+141 [Wei ZH, Xu J, Guo MX, Shi AM, Su JG. Research progress of carbendazim in China [J]. J Anhui Agric Sci 2015, 43 (3): 125-127+141]
15 赵双双, 潘洁莉, 刘巧, 蒋福升, 陈开, 丁志山, 李美芽. 农用真菌抑菌剂对白及种子萌发及小苗生长分化的影响[J]. 浙江农业科学,2017, 58 (4) : 638-641 [Zhao SS, Pan JL, Liu Q, Jiang FS, Chen K, Ding ZS, Li MY. Effects of agricultural fungal inhibitors on seed germiniation and seeding growth and differentiation of Bletilla striata [J]. Zhejiang Agric Sci, 2017, 58 (4) : 638-641]
16 王斯彤. 小叶朴组织培养无菌体系的建立[J]. 安徽农业科学, 2016,44 (31): 153-155 [Wang ST. The establishment of tissue culture sterile system of Celtis bungenana [J]. J Anhui Agric Sci, 2016, 44 (31): 153-155]
17 Ikeuchi M, Ogawa Y, Iwase A, Sugimoto K. Plant regeneration: cellular origins and molecular mechanisms [J]. Development, 2016, 143 (9): 1442-1451
18 钱子刚. 药用植物组织培养[M]. 北京: 中国中医药出版社, 2007: 30-31 [Qian ZG. Tissue Culture of Medicinal Plants [M]. Beijing: China Press of Traditional Chinese Medicine, 2007: 30-31]
19 吕娟, 龙秋梅, 普建春. 不同植物生长调节剂对草莓组培苗快繁的影响[J]. 现代农业科技, 2017, 21: 121+125 [Lü J, Long QM, Pu JC. Effect of different growth regulators on tissue culture and rapid propagation of strawberry [J]. Mod Agric Sci Technol, 2017, 21: 121+125]
20 赵展平, 何芳, 唐军荣, 罗旭璐, 赵平, 黄荐, 丁勇. 樟叶越桔组培苗生根和移栽技术研究[J]. 广西植物, http://kns.cnki.net/kcms/detail/45.1134.q.20181208.0956.002.html [Zhao ZP, He F, Tang JR, Luo XL, Zhao P, Huang J, Ding Y. Rooting and transplanting techniques of tissue culture plantlets of Vaccinium dunalianum [J]. Guihaia, http://kns.cnki.net/kcms/detail/45.1134.q.20181208.0956.002.html]
21 Abdulaziz M, Bahrany AI. Effect of phytohormones on in vitro shoot multiplication and rooting of lime Citrus aurantifolia (Christm.) Swing [J]. Sci Hort-Amsterdam, 2002, 95 (4): 285-295
22 AI-Juboory KH, Skirvin RM, Williams DJ. Callus induction and adventitious shoot regeneration of gardenia (Gardenia jasminoides Ellis) leaf explants [J]. Sci Hort-Amsterdam, 1998, 72 (3-4): 171-178
23 Fracro F, Echeverrigaray S. Micropagation of Cunila galioides, a popular medicinal plant of South Brazil [J]. Plant Cell Tiss Organ Cult, 2001, 64 (1): 1-4
24 张琳娜, 何俊, 张翔, 孟静. 弥勒苣苔组培苗生根及移栽基质的筛选 [J]. 西部林业科学, 2018, 47 (4): 69-73 [Zhang LN, He J, Zhang X, Meng J. Root induction and matrix optimization of Paraisometrum mileense tissue culture seedling [J]. J W Chin For Sci, 2018, 47 (4): 69-73]
25 徐航, 赵英, 韩晓燕, 牛建新. 沙棘组培苗瓶外生根技术研究[J]. 经济林研究, 2018, 36 (3): 182-186 [Xu H, Zhao Y, Han XY, Niu JX. Research of Hippophae rhamnoides plantlets ex vitro rooting technique [J]. Non-wood For Res, 2018, 36 (3): 182-186]
26 董林娜, 路群, 周根余. 北美红杉无性快繁技术的研究[J]. 上海农业学报, 2005 , 21 (4): 67-70 [Dong LN, Lu Q, Zhou GY. Study on technique of rapid asexual propagation of Sequoia sempervirens Lindl [J]. Acta Agric Shanghai, 2005, 21 (4): 67-70]

更新日期/Last Update: 2019-10-25