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[1]马涛,赵敏,王成业,等.美洲大蠊可培养真菌多样性[J].应用与环境生物学报,2019,25(05):1145-1153.[doi:10.19675/j.cnki.1006-687x.2018.10026]
 MA Tao,ZHAO Min,WANG Chenye & FENG Ying**.Diversity of cultivable fungi isolated from Periplaneta americana[J].Chinese Journal of Applied & Environmental Biology,2019,25(05):1145-1153.[doi:10.19675/j.cnki.1006-687x.2018.10026]
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美洲大蠊可培养真菌多样性
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年05期
页码:
1145-1153
栏目:
研究论文
出版日期:
2019-10-31

文章信息/Info

Title:
Diversity of cultivable fungi isolated from Periplaneta americana
作者:
马涛赵敏王成业冯颖
中国林业科学研究院,资源昆虫研究所 昆明 650233
Author(s):
MA TaoZHAO Min WANG Chenye & FENG Ying**
Research Institute of Resource Insects, Chinese Academy of Forestry, Kunming 650233, China
关键词:
酵母菌丝状真菌美洲大蠊饲养野生安全风险
Keywords:
yeast filamentous fungi Periplaneta americana reared wild risk of safety
分类号:
Q969.253.1 : Q949.320.8
DOI:
10.19675/j.cnki.1006-687x.2018.10026
摘要:
为了解养殖环境下美洲大蠊携带真菌情况、促进其饲养和利用中真菌的卫生安全防控,以饲养和野生样品为材料,分离培养其体表和体内真菌,基于菌落形态,通过ITS rDNA片段测序和聚类分析进行菌种鉴定,并分析不同环境下美洲大蠊体表和体内真菌多样性和相似性. 研究共分离鉴定211个菌株,划分为63个OTUs,至少隶属45属27科18目,以子囊菌门和担子菌门为主,其中酵母菌占绝对优势(76.8%),丝状真菌占分离菌株的23.2%. 野生环境下美洲大蠊的真菌数量和多样性(131株,Shannon-Wiener指数H = 3.391)均高于饲养环境(80株,H = 3.140),二者物种相似度较低(Sorenson系数为0.3421);无论饲养还是野生其体表真菌数量和多样性均高于体内,体内真菌物种半数以上(饲养61.1%,野生57.9%)在体表同时检出,其中假丝酵母属、链状假丝酵母、尼泊尔德巴利酵母和哈萨克斯坦酵母属4个类群分布最广,在饲养和野生美洲大蠊的体表和体内均有检出. 上述结果表明,美洲大蠊携带真菌与其生长环境具有相关性,控制饲养环境可降低虫体带菌数量;野生和饲养美洲大蠊体表体内均检出多种与条件致病菌和产毒真菌相关的物种,开发利用时应加以重视. (图4 表3 参37)
Abstract:
Fungi carried by Periplaneta americana have mainly been studied in terms of the health problems they may cause as pests. To date, there have been no reported studies on fungi carried by artificially reared P. americana. In this study, the fungi carried by artificially reared P. americana were explored to provide a theoretical basis and reference for the prevention and control of fungi, to enable the safe breeding and utilization of cockroaches. Reared and wild P. americana were collected and fungal strains were isolated from the external surface and internal parts of the insects. Isolates were identified based on rDNA ITS sequencing and cluster analysis. Fungal diversity was compared for different parts of the insect body and different habitats of the insect. A total of 211 strains of fungi were isolated and grouped into 63 operational taxonomic units (OTUs), which belonged to at least 45 genera, 27 families, and 18 orders under Ascomycota, Basidiomycota, and Zygomycota phyla. The majority of these strains belonged to Ascomycota and Basidiomycota phyla. Among the identified strains, 76.8% were yeasts, grouped into 37 OTUs, and 23.2% were filamentous fungi, grouped into 26 OTUs. Both the quantity and diversity of fungi associated with P. americana varied according to its living environment. One hundred and thirty-one strains were isolated from insects in their natural environment, with a Shannon-Wiener index of 3.391, whereas 80 strains were isolated from the reared insects, with a Shannon-Wiener index of 3.140. Moreover, the similarity between living environments was low, with a Sorenson coefficient of In both the natural and artificial rearing environment, more fungal strains and a higher diversity of fungi were obtained from the insect body surface than from its internal parts. More than half of the fungal species identified in internal parts (wild 57.9%, reared 61%) were also detected on the body surface. Candida, Diutina catenulata, Debaryomyces nepalensis, and Kazachstania are the most prevalent groups detected in the external and internal parts of P. americana from both artificial and natural environments. Our results revealed the great potential of P. americana for carrying fungi. The correlation between fungi carried by P. americana and its living environment suggested that controlling the feeding environment may reduce the safety risks associated with rearing these insects. A large variety of fungal species from both wild and reared insects were possible opportunistic pathogens and toxic or spoilage fungi. This requires serious consideration during the utilization of P. americana.

参考文献/References:

1. 吴福祯. 中国大蠊属的几种蜚蠊及其分布、生活习性与经济重要性(蜚蠊目: 蜚蠊科) [J]. 昆虫学报, 1982, 25 (4): 416-422 [Woo FC. Species of the genus Periplaneta Burmeister from China, with reference to their bionomics and economic importance (Blattaria: Blattidae) [J]. Acta Entomol Sin, 1982, 25 (4): 416-422]
2. Nasirian H. Infestation of cockroaches (Insecta: Blattaria) in the human dwelling environments: a systematic review and meta-analysis [J]. Acta Trop, 2017, 167: 86-98
3. Fotedar R, Shriniwas UB, Verma A. Cockroaches (Blattella germanica) as carriers of microorganisms of medical importance in hospitals [J]. Epidemiol Infect, 1991, 107 (1): 181-187
4. Salehzadeh A, Tavacol P, Mahjub H. Bacterial, fungal and parasitic contamination of cockroaches in public hospitals of Hamadan, Iran [J]. J Vector Dis, 2007, 44 (2): 105-110
5. Nasirian H. Contamination of cockroaches (Insecta: Blattaria) to medically fungi: a systematic review and meta-analysis [J]. J Mycol Med, 2017, 27 (4): 427-448
6. 陈伟, 郑义延, 吴珍泉. 室内人工繁殖美洲大蠊的产卵习性[J]. 福建农林大学学报(自然科学版), 2004, 33 (3): 318-321 [Chen W, Zheng YY, Wu ZQ. The egg laying habit of American cockroach (Periplaneta americana L.) in laboratory [J]. J Fujian Agric For Univ (Nat Sci Ed), 2004, 33 (3): 318-321]
7. 戴云, 曾茗, 项朋志.蜚蠊的药用价值[J]. 中药材, 2005, 28 (9): 848-850 [Dai Y, Zeng M, Xiang PZ. Medicinal value of cockroaches [J]. J Chin Med Mater, 2005, 28 (9): 848-850]
8. 张李香, 吴珍泉. 饲料营养成分对美洲大蠊雌成虫取食和生殖的影响[J]. 福建农林大学学报 (自然科学版), 2006, 35 (3): 239-242 [Zhang LX, Wu ZQ. Effects of forage nutritional components on the feeding and reproduction of the female adults of American cockroach[J]. J Fujian Agric For Univ (Nat Sci Ed), 2006, 35 (3): 239-242]
9. 刘昊, 李忠荣, 刘景, 王长康, 周琼. 美洲大蠊对肉鸡生产性能、血液生化指标及小肠绒毛高度的影响[J]. 北京农学院学报, 2009, 24 (1): 28-32 [Liu H, Li ZR, Liu J, Wang CK, Zhou Q. Effects of Periplaneta americana on growth performance, serum biochemical parameters and length of small intestinal villus in broilers [J]. J Beijing Univ Agric, 2009, 24 (1): 28-32]
10. 史未名. 美洲大蠊的药用研究进展[J]. 中国民族民间医药, 2012, 21 (3): 50-51 [Shi WM. The medical research advanced on Periplaneta americana [J]. Chin J Ethnomed Ethnopharm, 2012, 21 (3): 50-51]
11. 张明海, 王钰龙, 张风祥. 蟑螂规模化养殖获得成功[J]. 农业知识, 2013 (12): 25-25 [Zhang MH, Wang YL, Zhang FX. Success achieved in large-scale breeding cockroaches [J]. Agric Knowl, 2013 (12): 25-25]
12. 冯颖, 陈晓鸣, 赵敏. 中国食用昆虫[M]. 北京: 科学出版社, 2016:287-291 [Feng Y, Chen XM, Zhao M. Edible Insects of China [M]. Beijing: Science Press, 2016: 287-291]
13. 周琼, 吴珍泉, 李忠荣, 林虬, 刘景, 王长康. 美洲大蠊的营养价值与饲用安全性的分析[J]. 福建农业学报, 2007, 22 (3): 276-278 [Zhou Q, Wu ZQ, Li ZR, Lin Q, Liu J, Wang CK. Nutritive values and feeding safety of Periplaneta americana L. [J]. Fujian J Agric Sci, 2007, 22 (3): 276-278]
14. 袁东强, 何钊, 孙龙, 李娴, 赵敏, 冯颖. 美洲大蠊油脂的提取及性质分析[J]. 中国粮油学报, 2016, 31 (3): 84-90 [Yuan DQ, He Z, Sun L, Li X, Zhao M, Feng Y. Periplaneta americana fat extraction and properties analysis [J]. J Chin Cereal Oil Ass, 2016, 31 (3): 84-90]
15. 李娴, 何钊, 丁伟峰, 张欣, 孙龙, 冯颖. 美洲大蠊油脂对过氧化氢诱导sh-sy5y细胞氧化损伤的保护作用[J]. 环境昆虫学报, 2018, 40 (1): 36-42 [LI X, HE Z, Ding WF, Zhang X, Sun L, Feng Y. The protective effects of Periplaneta americana(Blattaria: Blattidae) oil on oxidative damage in SH-SY5Y cells induced by H2O2 [J]. J Environ Entomol, 2018, 40 (1): 36-42]
16. 孙龙, 冯颖, 何钊, 陈智勇, 陈晓鸣. 蟑螂水溶性多糖提取、分析及免疫活性研究[J]. 林业科学研究, 2009, 22 (2): 256-261 [Sun L, Feng Y, He Z, Chen ZY, Chen XM. Study on extraction, analysis of water-soluble polysaccharide from cockroaches and its immunologic activitie [J]. For Res, 2009, 22 (2): 256-261]
17. 王奎, 冯颖, 何钊, 孙龙, 陈智勇. 美洲大蠊中两个有抑菌活性的单甘酯的研究[J]. 时珍国医国药, 2013, 24 (9): 2102-2103 [Wang K, Feng Y, He Z, Sun L, Chen ZY. Study on two antimicrobial monoglycerides of Periplaneta americana L. [J]. Lishizhen Med Mater Med Res, 2013, 24 (9): 2102-2103]
18. 王奎, 冯颖, 孙龙,何钊, 陈智勇. 美洲大蠊乙酸乙酯提取物的分离和抑菌活性分析[J]. 林业科学研究, 2013, 26 (2): 163-166 [Wang K, Feng Y, Sun L, HE Z, Chen ZY. Isolation of ethyl acetate extract from Periplaneta americana and its antimicrobial activity [J]. For Res, 2013, 26 (2): 163-166]
19. 晋方佑,李治滢, 王永霞, 董明华, 严亚萍, 李绍兰, 杨丽源.云南程海湖可培养真菌多样性分析[J]. 应用与环境生物学报, 2013, 19 (6): 1025-1030 [Jin FY, Li ZY, Wang YX, Dong MH, Yan YP, Li SL, Yang LY. Diversity of cultivable fungi isolated from the Chenghai Lake in Yunnan province [J]. Chin J Appl Environ Biol, 2013, 19 (6): 1025-1030]
20. Lemos AA, Lemos JA, Prado MA, Pimenta FC, Gir E, Silva HM, Silva MRR. Cockroaches as carriers of fungi of medical importance [J]. Mycoses, 2006, 49 (1): 23-25
21. Isaac C, Orue PO, Iyamu MI, Ehiaghe JI, Isaac O. Comparative analysis of pathogenic organisms in cockroaches from different community settings in edo state, Nigeria [J]. Korean J Parasitol, 2014, 52 (2): 177-181
22. Haghi SFM, Aghili SR, Gholami S, Salmanian B, Nikokar SH, Geravi MK, Hajati H. Isolation of medically important fungi from cockroaches trapped at hospitals of Sari, Iran [J]. Bull Environ Pharmacol Life Sci, 2014, 3: 29-36
23. Davari B, Hassanvand AE, Nasirian H, Ghiasian SA, Salehzadeh A, Nazari M. Comparison of cockroach fungal contamination in the clinical and non-clinical environments from Iran [J]. J Entomol Acarol Res, 2017, 49 (2): 109-115
24. Kassiri H, Zarrin M, Veys-Behbahani R. Pathogenic fungal species associated with digestive system of Periplaneta americana (Blattaria: Blattidae) trapped from residential dwellings in Ahvaz city, southwestern Iran [J]. J Arthropod Borne Dis, 2018, 12 (1): 16-23
25. Schoch CL, Seifert KA, Huhndorf S, Robert V, Spouge JL, Levesque CA, Chen W, Fungal Barcoding Consortium. Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi [J]. PNAS, 2012, 109 (16): 6241-6246
26. 张宇, 郭良栋. 真菌DNA条形码研究进展[J]. 菌物学报, 2012, 31 (5): 809-820 [Zhang Y, Guo LD. Progress of fungal DNA barcode [J].Mycosystema, 2012, 31 (5): 809-820]
27. James MJ, Martin JL, David AG. 现代食品微生物学[M]. 7版. 北京: 中国农业大学出版社, 2008: 592-607 [James MJ, Martin JL, David AG. Modern Food Microbiology [M]. 7th ed. Beijing: China Agricultural university Press, 2008: 592-607]
28. Garnier L, Valence F, Mounier J. Diversity and control of spoilage fungi in dairy products: an update [J]. Microorganisms, 2017, 5 (3): pii E42
29. 陈涛, 刘嘉蓉, 刘勇, 童骁. 我国家居蜚蠊细菌的研究[J]. 微生物学通报, 1997, 24 (6): 347-349 [Chen T, Liu JR, Liu Y, Tong X. Studies on the bacteria of domestic cockroaches on China [J]. Microbiol China, 1997, 24 (6): 347-349]
30. Pai HH, Chen WC, Peng CF. Cockroaches as potential vectors of nosocomial infections [J]. Infect Control Hosp Epidemiol, 2004, 25 (11): 979-984
31. van Huis, A. Potential of insects as food and feed in assuring food security [J]. Annu Rev Entomol, 2013, 58: 563-583
32. Seabrooks L, Hu L. Insects: an underrepresented resource for the discovery of biologically active natural products [J]. Acta Pharm Sin B, 2017, 7 (4): 409-426
33. Feng Y, Chen XM , Zhao M , He Z, Sun L, Wang CY, Ding WF. Edible insects in China: Utilization and prospects [J]. Insect Sci, 2018, 25: 184-198
34. EFSA Scientific Committee. Risk profile related to production and consumption of insects as food and feed [J]. EFSA J, 2015, 13 (10): 4257
35. Grabowski NT, Klein G. Microbiological analysis of raw edible insects [J]. J Insects Food Feed, 2017, 3 (1):7-14
36. 赵敏, 冯颖, 何钊, 马涛, 王成业, 孙龙, 王金迪. 我国昆虫食用安全的研究进展[J]. 中国蚕业, 2018, 39 (2): 35-41 [Zhao M, Feng Y, He Z, Ma T, Wang CY, Sun L, Wang JD. Research advances of insects for food safety in China [J]. Ser China, 2018, 39 (2): 35-41]
37. Marshall DL, Dickson JS, Nguyen NH. Ensuring food safety in insect based foods: mitigation microbiological and other foodborne hazards [M]//Dossey AT, Morales-Ramos JA, Rojas MG. Insects as Sustainable Food Ingredients: Production, Processing and Food Applications. San Diego, CA, USA: Academic Press, 2016: 223-253

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更新日期/Last Update: 2019-10-25