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[1]李海红,佟欣宇,宦臣臣,等.高效异养硝化-好氧反硝化菌株TS-1筛选及降解特性[J].应用与环境生物学报,2020,26(04):791-799.
 LI Haihong,TONG Xinyu,HUAN Chenchen & YAN Zhiying.Characterizing the heterotrophically nitrifying and aerobically denitrifying TS-1 strain[J].Chinese Journal of Applied & Environmental Biology,2020,26(04):791-799.
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高效异养硝化-好氧反硝化菌株TS-1筛选及降解特性()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
26卷
期数:
2020年04期
页码:
791-799
栏目:
工业与环境微生物功能研究专栏
出版日期:
2020-08-25

文章信息/Info

Title:
Characterizing the heterotrophically nitrifying and aerobically denitrifying TS-1 strain
作者:
李海红佟欣宇宦臣臣闫志英
1西安工程大学环境与化学工程学院 西安 710000 2中国科学院成都生物研究所 成都 610041
Author(s):
LI Haihong1 TONG Xinyu1 HUAN Chenchen2 & YAN Zhiying2?
1 College of Environmental and Chemical Engineering, Xi’an Polytechnic University, Xi’an 710000, China 2 Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China
关键词:
异养硝化好氧反硝化Paracoccus denitrificans混合氮源生物脱氮
Keywords:
heterotrophic nitrification aerobic denitrification Paracoccus denitrificans mixed nitrogen source biological nitrogen removal
摘要:
从实验室定向驯化的活性污泥中分离筛选出一株具有异养硝化-好氧反硝化功能的菌株TS-1. 通过生理生化及16S rRNA基因序列鉴定其为脱氮副球菌,通过单因素和正交实验对其去除NH4+-N的最佳条件进行优化,并通过对比进一步探究其在不同氮源条件下对各形态无机氮的去除规律. 结果表明该菌株最适碳源为丁二酸钠,最佳C/N为15,最佳接种量为5%,最适温度为30 ℃、pH为8.0. 以初始浓度约为100 mg/L的NH4+-N、NO3--N和NO2--N分别为单一氮源时,菌株TS-1对各形态氮的去除率为97.49%、100%和95.94%;维持各形态氮初始浓度不变,将其两两混合时发现混合氮源中若包含NO2--N会使菌株OD600值达到最大值所用时间延长,氮源中含有NH4+-N会降低菌株对其他形态氮源的去除率,以及NO3--N的添加会使菌株对NH4+-N的去除能力降低;3种形态氮源同时存在的条件下,该菌对各氮源去除能力由强至弱为NO2--N > NH4+ -N > NO3--N. 本研究从活性污泥中分离筛选出一株具有高效异养硝化-好氧反硝化功能的菌株TS-1,通过研究碳源、氮源、温度、pH得到了最佳降解条件,可为废水短程脱氮提供参考. (图9 表4 参37)
Abstract:
In this study, the TS-1 strain with its heterotrophic nitrification and aerobic denitrification functions was isolated from an activated sludge that was acclimated in a laboratory. According to its physiological and biochemical characteristics as well as the 16S rRNA gene sequence, it was identified as Paracoccus denitrificans. Through single factor and orthogonal experiments, the NH4+-N removal conditions were optimized, while comparing the different forms and sources of inorganic nitrogen. The strain’s optimal carbon source, C/N ratio, inoculation amount, temperature, and pH were identified as sodium succinate, 15, 5%, 30 ℃, and 8.0, respectively. In addition, the individual removal rates of NH4+-N, NO3--N, and NO2--N by the TS-1 strain using an initial concentration of approximately 100 mg/L, were 97.49%, 100%, and 95.94%, respectively. When the initial concentration of the nitrogen forms was unchanged, but NO2--N was included, the time required for the strain to reach maximum optical density at 600 nm was prolonged, while including NH4+-N reduced the removal rate of other nitrogen sources using pressure, but adding NO3--N reduced this ability. Under the condition that the three forms of nitrogen can exist simultaneously, the bacteria’s removal ability of each nitrogen source was NO2--N > NH4+-N > NO3--N. Hence, the highly efficient heterotrophically nitrifying and aerobically denitrifying TS-1 strain was successfully isolated from an activated sludge, in which the optimal degradation conditions were identified by examining the carbon source, nitrogen source, temperature, and pH, to provide a reference for the short-range denitrification of wastewater.

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更新日期/Last Update: 2020-08-25