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[1]余金阳,黄潇慧,帅正彬,等.四川彭州大蒜根腐病发病土壤细菌与真菌群落结构[J].应用与环境生物学报,2020,26(04):928-935.
 YU Jinyang,HUANG Xiaohui,SHUAI Zhengbin,et al.The community structure of bacteria and fungi in soils with root rot diseased garlic plants in Pengzhou, Sichuan Province[J].Chinese Journal of Applied & Environmental Biology,2020,26(04):928-935.
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四川彭州大蒜根腐病发病土壤细菌与真菌群落结构()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
26卷
期数:
2020年04期
页码:
928-935
栏目:
研究论文
出版日期:
2020-08-25

文章信息/Info

Title:
The community structure of bacteria and fungi in soils with root rot diseased garlic plants in Pengzhou, Sichuan Province
作者:
余金阳黄潇慧帅正彬解亮刘轶豪王超仁柴丹刘蝶周晓宇陈强
1四川农业大学资源学院 成都 611130 2成都市农林科学院园艺研究所 成都 611130
Author(s):
YU Jinyang1 HUANG Xiaohui1 SHUAI Zhengbin2 XIE Liang1 LIU Yihao1 WANG Chaoren1 CHAI Dan2 LIU Die1 ZHOU Xiaoyu1 & CHEN Qiang1?
1College of Resources, Sichuan Agricultural University, Chengdu 611130, China 2Institute of Horticulture, Chengdu Academy of Agriculture and Forestry Sciences, Chengdu 611130, China
关键词:
大蒜根腐病土壤微生物群落高通量测序
Keywords:
garlic root rot soil microbial community high-throughput sequencing technology
摘要:
为了解土壤微生物群落与大蒜根腐病之间的关系,采集四川省彭州市不同地点大蒜正常生长和发病土壤样品,采用Illumina MiSeq测序技术分析土壤细菌与真菌的群落结构变化. 结果显示,供试土壤的优势细菌主要为变形菌门和绿弯菌门,优势真菌主要为子囊菌门和担子菌门;与正常土壤相比,发病土壤细菌多样性减少,真菌多样性和丰富度增加,其中硝化螺菌属、Nitrosomonadaceae-uncultured、芽孢杆菌属和鞘氨醇单胞菌属等有益细菌及青霉属、木霉属和热霉属等有益真菌丰度降低,匐柄霉属和镰刀菌属等病原真菌丰度增加. Spearman分析结果显示,匐柄霉属和镰刀菌属等病原真菌的相对丰度与土壤pH呈负相关,与土壤速效钾含量呈正相关. 本研究表明彭州大蒜根腐病发病与土壤微生物群落结构改变、土壤微生态失衡有关,结果对探明病害的发生机理具有现实意义,可为有效防治大蒜根腐病提供理论依据. (图2 表5 参36)
Abstract:
To explore differences in the soil bacteria and fungi communities of healthy and root rot garlic soil in Pengzhou, healthy and root rot garlic soil samples from different sites were collected, and the microbial community structures were analyzed by Illumina Miseq sequencing technology. Proteobacteria and Chloroflexi were the dominant bacteria, and Ascomycota and Basidiomycota were the dominant fungi. Compared to healthy soils, the bacterial diversity in garlic root rot soils decreased, while the fungal diversity and abundance increased. At the genus level, the abundance of beneficial bacteria (Nitrospira, Nitrosomonadaceae_uncultured, Bacillus, and Sphingomonas) and beneficial fungi (Penicillium, Trichoderma, and Thermomyces) decreased in garlic root rot soil, while the abundance of pathogenic fungal species (Stemphylium and Fusarium) increased. Spearman analysis showed that the abundance of pathogenic fungi was negatively correlated with soil pH, but was positively correlated with available potassium in the soil. These results suggest that the incidence of garlic root rot is related to variations in the Pengzhou soil microbial community structure and micro-ecological imbalances.

参考文献/References:

1 贾茹, 张迪, 马晓东, 姜佰文. 大蒜连作障碍研究进展[J]. 北方园艺, 2014, 19: 207-210 [Jia R, Zhang D, Ma XD, Jiang BW. Research progress of continuous cropping obstacle of garlic [J]. N Hort, 2014, 19: 207-210]
2 张丽娟, 茆军, 张志东, 谢玉清. 新疆大蒜根腐型病害根际土壤微生物群落多样性初探[J]. 新疆农业科学, 2013, 50 (11): 2109-2117 [Zhang LJ, Mao J, Zhang ZD, Xie YQ. Study of microbial community diversity in rhizosphere soil from garlic infected by root rot disease [J]. Xinjiang Agric Sci, 2013, 50 (11): 2109-2117]
3 毕武, 陈娟, 焦晓林, 高微微. 北京地区西洋参根腐病病原鉴定及其致病性[J]. 植物保护, 2011, 37 (5): 135-138 [Bi W, Chen J, Jiao XL, Gao Weiwei. Identification of the pathogens causing the root rot and their pathogenicity on American ginseng in Beijing [J]. Plant Prot, 2011, 37 (5): 135-138]
4 Golzar H, Phillips D, Mack S. Occurrence of strawberry root and crown rot in Western Australia [J]. Australas Plant Dis Notes, 2007, 2 (1): 145-147
5 张丽娟, 王玮, 谢玉清, 张志东, 顾美英, 朱静, 唐琦勇, 王博, 宋素琴. 新疆吉木萨尔地区大蒜根腐病病原菌的分离与鉴定[J]. 新疆农业科学, 2017, 54 (4): 725-734 [Zhang LJ, Wang W, Xie YQ, Zhang ZD, Gu MY, Zhu J, Tang QY, Wang B, Song SQ. Isolation and identification of the pathogens causing garlic root rot in Jimsar [J]. Xinjiang Agric Sci, 2017, 54 (4): 725-734]
6 张博. 山东省大蒜腐霉根腐病初步研究[D]. 乌鲁木齐: 新疆农业大学, 2008 [Zhang B Preliminary study on the pythium root rot of garlic in Shandong province [D]. Urumq: Xinjiang Agricultural University, 2008]
7 楼骏, 柳勇, 李延. 高通量测序技术在土壤微生物多样性研究中的研究进展[J]. 中国农学通报, 2014, 30 (15): 256-260 [Lou J, Liu Y, Li Y. Review of high-throughput sequencing techniques in studies of soil microbial diversity [J]. Chin Agric Sci Bull, 2014, 30 (15): 256-260]
8 余妙, 蒋景龙, 任绪明, 李丽, 焦成瑾, 杨玲娟, 徐皓. 西洋参根腐病发生与根际真菌群落变化关系研究[J]. 中国中药杂志, 2018, 43 (10): 2038-2047 [Yu M, Jiang JL, Ren XM, Li L, Jiao CJ, Yang LJ, Xu H. Research on relationship between occurrence of root rot and changes of fungal communities in rhizosphere of Panax quinquefolius [J]. J Tradit Chin Med, 2018, 43 (10): 2038-2047]
9 Tan Y, Cui Y, Li H. Rhizospheric soil and root endogenous fungal diversity and composition in response to continuous Panax notoginseng cropping practices [J]. Microbiol Res, 2016, 194: 10-19.
10 宋旭红, 谭均, 李隆云, 王钰, 伍晓丽. Illumina高通量测序揭示黄连根腐根际土壤真菌群落组成及多样性[J]. 中草药, 2018, 49 (22): 5396-5403 [Song XH, Tan J, Li LY, Wang Y, Wu XL. Illumina high-throughput sequencing reveals fungal community composition and diversity in root rot of Coptis chinensis in rhizosphere soil [J]. Chin Tradit Herb Drugs, 2018, 49 (22): 5396-5403]
11 鲁如坤. 土壤农业化学分析方法[M]. 北京: 中国农业科学出版社, 1999: 296-338 [Lu RK. Methods of Soil Agricultural Chemistry Analysis [M]. Beijing: Chinese Agricultural Science and Technology Press, 1999: 296-338]
12 Salzberg SL, Magoc T. FLASH: fast length adjustment of short reads to improve genome assemblies [J]. Bioinformatics, 2011, 27 (21): 2957-2963
13 Caporaso JG, Kuczynski J, Stombaugh J. QIIME allows analysis of high-throughput community sequencing data [J]. Nat Methods, 2010, 7 (5): 335-336.
14 Edgar RC. Search and clustering orders of magnitude faster than BLAST [J]. Bioinformatics, 2010, 26 (19): 2460
15 Blaxter M, Mann JT, Thomas F. Defining operational taxonomic units using DNA barcode data [J]. Int J Biol Sci, 2005, 360 (1462): 1935-1943
16 Urmas KL, R Henrik N, Kessy A. Towards a unified paradigm for sequence-based identification of fungi [J]. Mol Ecol, 2013, 22 (21): 5271-5277
17 Pitta DW, Parmar N, Patel AK, Indugu N, Prajapathi KB, Patel AB, Reddy B, Joshi C. Bacterial diversity dynamics associated with different diets and different primer pairs in the rumen of kankrej cattle [J]. PLoS ONE, 2014, 9 (11): e111710
18 王宝荣, 杨佳佳, 安韶山. 黄土丘陵区植被与地形特征对土壤和土壤微生物生物量生态化学计量特征的影响[J]. 应用生态学报, 2018, 29 (1): 247-259 [Wang BR, Yang JJ, An SS, Zhang HX, Bai XJ. Effects of vegetation and topography features on ecological stoichiometry of soil and soil microbial biomass in the hilly-gully region of the Loess Plateau, China [J]. Chin J Appl Ecol, 2018, 29 (1): 247-259]
19 Hinsinger P, Bengough AG, Vetterlein DI. Rhizosphere: biophysics, biogeochemistry and ecological relevance [J]. Plant Soil, 2009, 321 (1/2): 117-152
20 Liu J Wang FY. Effects of cinnamic acid on bacterial community diversity in rhizosphere soil of cucumber seedlings under salt stress [J]. Agric Sci Chin, 2010, 9 (2): 266-274
21 Qin, SH, Yeboah, Stephen, Cao L, Zhang, JL, Shi SL, Liu YH. Breaking continuous potato cropping with legumes improves soil microbial communities, enzyme activities and tuber yield [J]. PLoS ONE, 2017, 12 (5): e175934
22 Li SD, Miao ZQ, Gao WD. Challenges, opportunities and obligations in management of soilborne plant diseases in China [J]. Chin J Biol Control, 2011, 4: 433-440
23 林红梅, 施建飞, 李岳桦, 孙卓, 杨利民. 西洋参病原菌拮抗细菌的分离筛选与鉴定[J]. 吉林农业科学, 2013, 38 (6): 62-65 [Lin HM, Shi JF, Li YH, Sun Z, Yang LM. Isolation and identification of antagonistic bacteria of panax quinquefolium pathogeny fungus [J]. J Jilin Agric Sci, 2013, 38 (6): 62-65]
24 Li W, Liu Q, Chen P. Effect of long-term continuous cropping of strawberry on soil bacterial community structure and diversity [J]. J Integr Agric, 2018, 17 (11): 2570-2582.
25 Boughalleb-M Hamdi N, Salem I B, M Hamdi M. Evaluation of the efficiency of Trichoderma, Penicillium, and Aspergillus species as biological control agents against four soil-borne fungi of melon and watermelon [J]. Egypt J Biol Pest Control, 2018, 28 (1): 1-12
26 Lecomte C, Alabouvette C, Edel-Hermann V. Biological control of ornamental plant diseases caused by Fusarium oxysporum: a review [J]. Biol Control, 2016, 101: 17-30
27 康萍芝, 白小军, 张丽荣, 杜玉宁, 张治科. 尖孢镰刀菌黄瓜专化型生物学特性研究[J]. 北方园艺, 2018, 421 (22): 65-69 [Kang PZ, Bai XJ, Zhang LR, Du YN, Zhang ZK. Biological characteristics of Fusarium oxysporium (Schl.) f.sp.cucumerinum Owen [J]. N Hort, 2018, 421 (22): 65-69]
28 周艳芳, 王燕春, 赵伟强, 徐佳, 路培, 郑力嘉. 我国桔梗上一种新病害匍柄霉叶斑病的病原[J]. 菌物学报, 2018, 37 (4): 511-515 [Zhou YF, Wang CY, Zhao WQ, Xu J, Lu P, Zheng LJ. A new leaf spot disease of bellflower (Plotycodon grondiflorum) caused by Stemphylium plotycodontis in China [J]. Mycosystema, 2018, 37 (4): 511-515]
29 丁工尧,史德鹏,彭芳华. 苜蓿病害病原真菌种类及其病原菌研究[J]. 当代畜牧, 2016, 30: 46-47 [Ding GY, Shi DP, Peng FH. Study on the pathogenic fungi species and pathogenic bacteria of alfalfa diseases [J]. Contemp Anim Husb, 2016, 30: 46-47]
30 张志强. 大蒜叶枯病病原生物学及病菌毒素培养条件研究[D]. 杨凌: 西北农林科技大学, 2007 [Zhang ZQ. Study on the biology of pathogen and culture conditions of pathogen toxins of garlic tip blight [D]. Yangling: Northwest Agriculture and Forestry University, 2007]
31 Xu L, Ravnskov S, Larsen J. Soil fungal community structure along a soil health gradient in pea fields examined using deep amplicon sequencing [J]. Soil Biol Biochem, 2015, 46: 26-32
32 宋旭红, 王钰, 李隆云, 谭均. 石柱黄连根腐病根际土壤细菌微生态研究[J]. 中国中药杂志, 2017, 42 (7): 1304-1311 [Song XH, Wang Y, Li LY, Tan J. Research on bacteria microecology in root rot rhizosphere soil of Coptis chinensis produced in Shizhu city [J]. Chin J Chin Mater Med, 2017, 42 (7): 1304-1311]
33 漆永红, 曹素芳, 李雪萍, 李敏权. 甘南州临潭县青稞根际土壤养分含量、酶活性和微生物数量与根腐病的关系研究[J]. 草地学报, 2018, 26 (4): 877-884 [Qi YH, Cao SF, Li XP, Li MQ. Study on the relationship between root rot disease and nutrient content, enzyme activity and microbial quantity in rhizosphere soil of naked barley in lintan county, gannan state [J]. Acta Agrestia Sin, 2018, 26 (4): 877-884]
34 张建华, 杨发荣. 大理市蔬菜地土壤酸化的原因与调控措施[J]. 云南农业科技, 2004, 2: 4-6 [Zhang JH, Yang RF. Controling of soil acidization of vegetable land in Dali prefecture [J]. Yunnan Agric Sci Technol, 2004, 2: 4-6]
35 Zhang D, Meng KX, Hao YH. Comparative proteomic analysis of cucumber roots infected by Fusarium oxysporum f. sp. cucumerium Owen [J]. Physiol Mol Plant Pathol, 2016, 96: 77-84
36 Dang P, Yu X, Le H. Effects of stand age and soil properties on soil bacterial and fungal community composition in Chinese pine plantations on the Loess Plateau [J]. PLoS ONE, 2017, 12 (10): e186501

更新日期/Last Update: 2020-08-25