|本期目录/Table of Contents|

[1]陈杰,许长征,曹颖倩,等.不同重金属对拟南芥根系特征的影响比较[J].应用与环境生物学报,2017,23(06):1122-1128.[doi:10.3724/SP.J.1145.2017.03003]
 CHEN Jie,XU Changzheng,CAO Yingqian,et al.Heavy-metal-induced morphological changes of root characteristics in Arabidopsis thaliana[J].Chinese Journal of Applied & Environmental Biology,2017,23(06):1122-1128.[doi:10.3724/SP.J.1145.2017.03003]
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不同重金属对拟南芥根系特征的影响比较()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
23卷
期数:
2017年06期
页码:
1122-1128
栏目:
研究论文
出版日期:
2017-12-25

文章信息/Info

Title:
Heavy-metal-induced morphological changes of root characteristics in Arabidopsis thaliana
作者:
陈杰 许长征 曹颖倩 李菁 罗锋 雷波
1西南大学生物能源与环境修复中心 重庆 400715 2西南大学生命科学学院 重庆 400715 3重庆市环境科学研究院 重庆 400020
Author(s):
CHEN Jie1 XU Changzheng2 CAO Yingqian1 LI Jing1 LUO Feng1** & LEI Bo3**
1Research?Center?of Bioenergy?&?Bioremediation,?Southwest?University,?Chongqing?400715,??China 2College of Life Sciences,?Southwest?University,?Chongqing?400715,?China 3Chongqing Academy of Environmental Science, Chongqing?400020,?China
关键词:
拟南芥重金属胁迫根系主成分分析
Keywords:
Arabidopsis?thaliana?heavy?metal stress?root?system PCA
分类号:
X173
DOI:
10.3724/SP.J.1145.2017.03003
摘要:
重金属胁迫严重影响着植物根系统发育,为了解不同重金属胁迫对植物根系系统的影响,选取模式植物拟南芥根系作为研究对象,选用镉(Cd)、镍(Ni)、铅(Pb)、铜(Cu)、砷(As)5种重金属(类重金属)元素进行胁迫处理,每种重金属分别设置5个浓度梯度,主要观察总根长(TR length)、初生根长(PR length)、一级侧根长(1°LR length)、一级侧根密度(1°LR dentisty)以及侧根未着生区占初生根长度的比值(BZ/PR)这5个指标的变化. 结果显示拟南芥根系在重金属胁迫下初生根根长生长受到明显抑制,低浓度的Cu和Pb能刺激侧根生长. 主成分分析(PCA)显示植物必需元素Cu和类重金属三价元素As分别在PC1和PC2上出现明显偏离. 结合根系特征数据分析,Cu、Cd、Pb、Ni元素胁迫时,随着环境中胁迫加剧侧根发生位置会向根尖移动,但是低浓度Cu元素胁迫时侧根生长受到轻微促进,一级侧根长度和一级侧根密度都有略微增加,而拟南芥根系在受到类重金属元素As胁迫时倾向于促进侧根生长. 上述结果表明不同重金属胁迫下拟南芥的侧根生长呈现不同的响应情况,这可为植物抗重金属胁迫研究提供思路. (图6 表1 参19)
Abstract:
The growth of a plant root system is seriously affected by heavy metal stress. However, the effects of different heavy metal stressors on the growth of a root system are different. In this study the roots of Arabidopsis thaliana, a herbaceous model plant, were treated with a heavy metal (Cd, Ni, Pb, Cu, or As) at five concentrations. Growth parameters of the roots including total root length, primary root length, lateral root length, lateral root density, and the length of the branching zone were measured. Principal component analysis (PCA) was performed to explore root morphological changes in response to different heavy metal stressors. Generally, the growth of the root system of A. thaliana was significantly inhibited under heavy metal stress. However, low-dose Cu or Pb stimulated the growth of lateral roots. The PCA results showed that Cu, an essential element for plants, and As, a metalloid without any physiological necessity, exhibited significant deviations from PC1 and PC2, respectively. Under Cu, Cd, Pb, or Ni stress, the position of lateral root formation moved to the root tip with the aggravating environmental stress. However, low-dose Cu treatment slightly stimulated the growth of lateral roots, and 1°LR length and 1°LR density increased slightly. As for As, this stressor tended to stimulate the growth of lateral roots. In comparison with three other heavy metals (Cd, Ni, and Pb), Cu and As had greater influences on the growth of lateral roots, representing a different strategy in response to heavy metal stress. The lateral roots of A. thaliana showed different responses to different heavy-metal stressors. These findings will benefit future research on heavy metal resistance in plants.

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更新日期/Last Update: 2017-12-25