|本期目录/Table of Contents|

[1]李筱筱,刘炜,咸漠,等.大肠杆菌乙酰酯酶(AES)的酶学性质[J].应用与环境生物学报,2017,23(06):1011-1014.[doi:10.3724/SP.J.1145.2016.12009]
 LI Xiaoxiao,LIU Wei,XIAN Mo & WANG Wei**.Characterization of Escherichia coli acetyl esterase (AES) activity[J].Chinese Journal of Applied & Environmental Biology,2017,23(06):1011-1014.[doi:10.3724/SP.J.1145.2016.12009]
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大肠杆菌乙酰酯酶(AES)的酶学性质()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
23卷
期数:
2017年06期
页码:
1011-1014
栏目:
微生物资源发掘与生物合成专栏论文
出版日期:
2017-12-25

文章信息/Info

Title:
Characterization of Escherichia coli acetyl esterase (AES) activity
作者:
李筱筱 刘炜 咸漠 王卫
1青岛科技大学化学与分子工程学院 青岛 266042 2中国科学院青岛生物能源与过程研究所 青岛 266101
Author(s):
LI Xiaoxiao1 LIU Wei2 XIAN Mo2 & WANG Wei1**
1 College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China 2 Qingdao Institute of Bioenergy and Bioprocess Technology, Qingdao 266101, China
关键词:
香叶醇乙酰酯酶(AES)大肠杆菌生物合成
Keywords:
geraniol acetyl esterase (AES) Escherichia coli biosynthesis
分类号:
Q936
DOI:
10.3724/SP.J.1145.2016.12009
摘要:
以乙酸香叶酯为底物,对大肠杆菌乙酰酯酶(Acetyl esterase,AES)的酶学性质进行解析,实现将乙酸香叶酯生物转化为香叶醇. 结果显示:大肠杆菌AES酶的最佳反应pH范围为6.0-7.5;最适反应温度为30 ℃;最佳催化时间为2 h;Mg2+、Mn2+、Co2+对酶活没有太大影响,而Cu2+、Zn2+明显抑制酶的活性. 对AES的动力学研究可知,其米氏常数Km、最大反应常数Vm分别为2.88 mmol/L、1.87 mmol/L. 该酶同样可水解乙酸松油酯、乙酸薄荷酯、乙酸香茅酯,分别产生松油醇、薄荷醇和香茅醇. 本研究得出了大肠杆菌乙酰酯酶的最适反应条件,可为后期香叶醇的生物合成优化和产量提高以及其他萜醇类化合物的生物合成提供参考依据. (图4 表1 参13)
Abstract:
The enzymatic properties of Escherichia coli acetyl esterase (AES) were analyzed using geranyl acetate as a substrate, and the biotransformation of geranyl acetate to geraniol was realized. The results show that the optimum reaction pH range of AES enzyme was 6.0-7.5, the optimal reaction temperature was 30 °C, and the optimal catalytic time was 2 h. Mg2+, Mn2+, and Co2+ had little impact on enzyme activity, whereas Cu2+ and Zn2+ noticeably inhibited enzyme activity. Kinetic studies demonstrated that the kinetic parameters Km and Vm of the enzyme were 2.88 mmol/L and 1.87 mmol/L respectively. The enzyme also hydrolyzed terpinyl acetate, mint acetate, and citronella acetate, and produced terpineol, menthol, and geraniol respectively. The study obtained optimal reaction conditions for the E. coli acetyl ester enzyme, which supports biosynthesis optimization and an increase in the output of geraniol, and provides theoretical guidance for the biosynthesis of other terpenoids.

参考文献/References:

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更新日期/Last Update: 2017-12-25