|本期目录/Table of Contents|

[1]张珩琳,杨婧,周浩,等.Cupriavidus metallidurans SHE胞内提取物合成纳米金及其催化应用[J].应用与环境生物学报,2019,25(02):457-462.[doi:10.19675/j.cnki.1006-687x.2018.07023]
 ZHANG Henglin,YANG Jing,ZHOU Hao,et al.Biosynthesis of gold nanoparticles by the cell-free extracts of Cupriavidus metallidurans SHE and its catalytic applications[J].Chinese Journal of Applied & Environmental Biology,2019,25(02):457-462.[doi:10.19675/j.cnki.1006-687x.2018.07023]

Cupriavidus metallidurans SHE胞内提取物合成纳米金及其催化应用()




Biosynthesis of gold nanoparticles by the cell-free extracts of Cupriavidus metallidurans SHE and its catalytic applications
大连理工大学环境学院,工业生态与环境工程教育部重点实验室 大连 116024
ZHANG Henglin YANG Jing ZHOU Hao LI Zheng LI Yan DAI Chunxiao & QU Yuanyuan**
Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China
纳米金Cupriavidus metallidurans生物合成4-硝基苯酚偶氮染料
gold nanoparticle Cupriavidus metallidurans biosynthesis 4-nitrophenol azo dyes
纳米金(AuNPs)凭借其在电化学和光学方面独特的性能,在催化、环境污染物的降解以及环境修复等方面近年来成为研究热点. 相比较传统的合成方法,生物法合成纳米金具有过程简单、无毒、环保、成本较低等优势;除此之外,生物法合成的纳米金颗粒具有尺寸均一、单分散性好且生物相容性好的特性. 利用Cupriavidus metallidurans SHE胞内提取物合成纳米金,结果显示最适反应条件为胞内提取物浓度为500 mg/L,HAuCl4浓度为1 mmol/L,pH为7,反应时间为7 d;透射电子显微镜(TEM)图像表明纳米金颗粒主要为球形和伪球形,平均粒径为14.3 nm;通过傅里叶转换红外线光谱(FTIR)分析结果推测提取物中的羟基、氨基、羧基等官能团参与了纳米金的稳定过程;在上述条件下合成的纳米金对4-硝基苯酚具有较好的催化还原活性,其催化速率常数k为5.98 × 10-4/s,且该纳米金能催化脱色多种偶氮染料. 本研究表明Cupriavidus metallidurans SHE能绿色合成尺寸均一、分散性良好的纳米金,且该纳米金在催化还原硝基芳烃污染物和偶氮染料方面具有潜在的应用价值. (图4 表1 参28)
Gold nanoparticles (AuNPs) with their unique properties of electrochemistry and photology have been widely used in catalysis, degradation of pollutants, environmental modification, and so on. Compared with conventional synthesis methods, AuNP biosynthesis is simpler, non-toxic, cleaner, eco-friendly, and cost effective. Furthermore, biosynthesized AuNPs have attracted much attention owing to the advantages of uniform particle size, single dispersion, and better biological capacity. In this study, a cell-free extract of Cupriavidus metallidurans SHE was used to synthesize AuNPs. The optimal conditions were 500 mg/L cell-free extract, 1 mmol/L HAuCl4, pH 7, and 7 days. Transmission electron microscope (TEM) images showed that the AuNPs were pseudospherical and spherical with an average size of 14.3 nm. Fourier transform infrared spectrometer (FTIR) analysis showed that hydroxyl, amino, and carboxyl groups played a role in the stability process. The investigation of catalytic properties proved that the AuNPs could serve as highly efficient catalysts for 4-nitrophenol reduction with a reaction rate constant k of 5.98 × 10-4/s. Additionally, it has potential for azo dye decolorization. This study showed that C. metallidurans SHE could synthesize AuNPs with good dispersion and the AuNPs had potential application in the catalytic reduction of 4-NP and azo dyes.


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[1]裴晓芳,沈文丽,由胜男,等.Trichosporon montevideense WIN提取物合成纳米金及其对硝基芳烃的催化特性[J].应用与环境生物学报,2017,23(03):409.[doi:2016.06004]
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 YANG Jing,LUAN Yunpeng,LU Zhiyu,et al.Influencing factors and catalytic characteristics of gold nanoparticles biosynthesized with the cell-free extracts of Trichoderma sp. WL-Go[J].Chinese Journal of Applied & Environmental Biology,2019,25(02):1451.[doi:10.19675/j.cnki.1006-687x.2019.01021]

更新日期/Last Update: 2019-04-25