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[1]王云,彭丽云,孙雪丽,等.龙眼Hsf基因家族全基因组鉴定及体胚发生过程中的表达分析[J].应用与环境生物学报,2019,25(02):420-431.[doi:10.19675/j.cnki.1006-687x.2018.06004]
　WANG Yun,PENG Liyun,SUN Xueli,et al.Genome-wide identification of longan Hsf family members and their functional analysis during somatic embryogenesis in longan[J].Chinese Journal of Applied & Environmental Biology,2019,25(02):420-431.[doi:10.19675/j.cnki.1006-687x.2018.06004]
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龙眼Hsf基因家族全基因组鉴定及体胚发生过程中的表达分析()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年02期
页码:
420-431
栏目:
研究论文
出版日期:
2019-04-25

文章信息/Info

Title:
Genome-wide identification of longan Hsf family members and their functional analysis during somatic embryogenesis in longan
作者:
王云; 彭丽云; 孙雪丽; 高玉莹; 陈晓慧; 陈裕坤; 林玉玲; 赖钟雄
福建农林大学园艺植物生物工程研究所 福州 350002
Author(s):
WANG Yun;  PENG Liyun;  SUN Xueli;  GAO Yuying;  CHEN Xiaohui;  LIN Yuling & LAI Zhongxiong**
Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
关键词:
龙眼; 热激转录因子家族; DlHsf成员鉴定; 表达分析; miRNA预测
Keywords:
Dimocarpus longan Lour.;  heat shock transcription factor family;  DlHsf gene identification;  expression analysis;  miRNA prediction
分类号:
S667.203
DOI:
10.19675/j.cnki.1006-687x.2018.06004
摘要:
基于龙眼基因组数据库对龙眼Hsf（DlHsf）基因家族进行全基因组鉴定，并对其基因结构、启动子作用元件和CpG岛分布情况、编码蛋白的理化性质和进化情况以及它们在不同体胚发生阶段和不同组织器官的表达情况进行研究，同时对靶定DlHsf的miRNA进行预测分析. 结果表明：DlHsf基因家族共20个成员，基因结构高度保守. 它们编码的蛋白具有典型的DNA结合域（DBD），且均为不含信号肽的不稳定蛋白. 启动子分析发现，该基因家族成员的启动子除典型的热激元件（HSE）外，还含有MYB元件等与逆境胁迫及生长发育相关的元件，DlHsfA1d、DlHsfA9a、DlHsfA9b和DlHsfC1启动子存在典型的CpG岛. 进化树分析表明DlHsf可分为DlHsfA、DlHsfB和DlHsfC三类. qRT-PCR结果表明，DlHsf在胚性阶段（EC）和球形胚阶段（GE）表达量较高，在不完全胚性紧实结构阶段（ICpEC）表达量较低，在龙眼体胚发育过程中呈现“V”状趋势，表明DlHsf可能主要在EC、GE阶段发挥作用. 此外，DlHsf家族成员在花芽和花蕾中表达量较高，其次是在果肉中，推测DlHsfs可能与龙眼花发育等生长过程相关. psRNA Target预测结果表明DlHsf基因家族受到miRNA调控，且调控方式多样. 本研究表明DlHsf功能多样，可能在龙眼生长发育、胁迫响应、DNA甲基化、组织器官特异表达等生物学过程中发挥作用. （图8 表5 参38）
Abstract:
This study identified and investigated the biological functions of the heat shock transcription factor (DlHsf) family in Dimocarpus longan Lour. Based on the longan genome database, DlHsf gene family members were screened, analyzed with their gene structure, promoter acting elements and CpG island distribution, physicochemical properties obtained, and evolution analysis of their encoded proteins. Their expression in different somatic embryogenesis stages and different tissues and organs was analyzed by quantitative real-time polymerase chain reaction (qRT-PCR). The miRNA targeting DlHsf family members were predicted and analysed by psRNA target. In total, 20 DlHsf gene family members were identified. The structures of the DlHsf were highly conserved. The encoding proteins of DlHsf were all unstable hydrophobic proteins with a DNA-binding domain and without signal peptides. In addition, all their promoters contained a heat-stress-related heat shock element, and some stress and growth and development-related elements, such as MYB-related elements, suggesting DlHsf were involved in a variety of stress and growth and development-related conditions. There were also typical CpG islands in DlHsfA1d, DlHsfA9a, DlHsfA9b, and DlHsfC1 promoters. Phylogenetic relationship analysis showed that the DlHsfs were mainly classified into three categories, i.e., DlHsfA, DlHsfB, and DlHsfC. The qRT-PCR results showed that the expression of DlHsf gene family members varied at different somatic embryogenesis stages and in different tissues and organs. Their expression levels in the embryogenic callus (EC) and globular embryos (GE) were higher than that in the incomplete compact pro-embryogenic cultures. With the development of longan somatic embryos, the expression of DlHsf showed a “V”-like curve, indicating that DlHsf may play a major role in the EC and GE stages during somatic embryogenesis. Higher expression levels in flower buds and alabastrums, followed by the flesh indicated that the DlHsf gene family members influenced flowering and other growth processes during the growth and development of longan tissues and organs. psRNA target results indicated that miRNA showed diverse regulation on DlHsf. DlHsf gene family members are versatile and may play a role in longan growth, stress response, DNA methylation and tissue-specific expression, and other important biological functions.

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更新日期/Last Update: 2019-04-25