|本期目录/Table of Contents|

[1]蒋佳宏,王东,胡源,等.拟南芥TAK蛋白激酶的结构预测与功能分析[J].应用与环境生物学报,2009,15(05):610-614.[doi:10.3724/SP.J.1145.2009.00610]
 JIANG Jiahong,WANG Dong,HU Yuan & DU Linfang.Structure Prediction and Function Analysis of TAK Kinase in Arabidopsis[J].Chinese Journal of Applied & Environmental Biology,2009,15(05):610-614.[doi:10.3724/SP.J.1145.2009.00610]
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拟南芥TAK蛋白激酶的结构预测与功能分析()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
15卷
期数:
2009年05期
页码:
610-614
栏目:
研究论文
出版日期:
2009-10-25

文章信息/Info

Title:
Structure Prediction and Function Analysis of TAK Kinase in Arabidopsis
作者:
蒋佳宏王东胡源杜林方
(1四川大学生物资源与生态环境教育部重点实验室 成都 610064)
(2四川大学纳米生物医学技术与膜生物学研究所 成都 610041)
Author(s):
JIANG Jiahong WANG Dong HU Yuan & DU Linfang
(1Key Laboratory of Ministry of Education for Bio-resources and Eco-environment, College of Life Sciences, Sichuan University, Chengdu 610064, China)
(2Institute for Nanobiomedical Technology and Membrane Biology, Sichuan University, Chengdu 610041, China)
关键词:
TAK磷酸化LHCII核心结构域同源建模抗体
Keywords:
TAK phosphorylation LHCII structure of core domain homologous modeling antibody
分类号:
Q946.5
DOI:
10.3724/SP.J.1145.2009.00610
文献标志码:
A
摘要:
TAK1、TAK2和TAK3属于TAK蛋白激酶家族,由拟南芥核基因编码,其中TAK1参与了主要捕光色素复合物LHCⅡ蛋白磷酸化调控与光系统的状态转移. 本文使用生物信息学手段对TAK1、TAK2和TAK3蛋白进行了较为系统的分析,发现TAK1、TAK2和TAK3为单次跨膜蛋白,具有保守的激酶活性域、疏水性强的N端跨越类囊体膜、亲水性高的C端处于基质中等特点. 在PDB中找到了不少与TAK1、TAK2和TAK3同源性大于30%的蛋白序列,其中大部分也带有酪氨酸激酶保守域,使用同源建模的方法,建立了拟南芥蛋白激酶TAKs核心结构域的三维结构,围绕蛋白激酶的结构和作用机制关系进行了探讨,设计出多肽抗体,并进行蛋白印迹检测抗体的专一性,为TAKs蛋白激酶进一步的功能研究奠定了基础. 图5 表1 参15
Abstract:
TAK1, TAK2 and TAK3 belong to TAK kinase family and are nuclear-encoded kinases. TAK1 is involved in the phosphorylation of LHCII and the state transitions. Few studies on the structure or function of TAKs have been done because they are membrane combined proteins. In this research, TAKs were studied using a systematical bioinformatical method and all the members of TAK kinase family were found with a hydrophobic N-terminal connected with a transmembrane region located in thylakoid membrane and a hydrophilic C-terminal (kinase catalytic domains) on the stromal side of thylakoid. Lots of sequences which contained the same TyrKc kinase catalytic domains with a similarity more than 30% were found in PDB, and three dimensional structures of core domains of TAK1, TAK2 and TAK3 were then predicted by using homologous modeling method. The analysis of the relationship between the structure and function of TAKs provided the foundation for further studying the functions of TAK1, TAK2 and TAK3. We designed an antibody on the base of prediction of the three dimensional structures, and its western blotting showed that there was an immunoaffinity reaction. Fig 5, Tab 1, Ref 15

参考文献/References:

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备注/Memo

备注/Memo:
教育部新世纪人才支持计划(No. NCET-04-0861)和四川大学“985”项目资助 Supported by the Program for New Century Excellent Talents in Universities of China (No. NCET-04-0861) and the Grant “985” of Sichuan University, China
更新日期/Last Update: 2009-10-26