|本期目录/Table of Contents|

[1]刘静,张道勇,潘响亮,等.藻菌生物膜胞外聚合物(EPS)与Al3+的配位作用机理[J].应用与环境生物学报,2009,15(03):347-350.[doi:10.3724/SP.J.1145.2009.00347]
 LIU Jing,ZHANG Daoyong,PAN Xiangliang,et al.Characterization of the Complexation Between Al3+ and Extracellular Polymeric Substances Prepared from Alga-bacteria Biofilm[J].Chinese Journal of Applied & Environmental Biology,2009,15(03):347-350.[doi:10.3724/SP.J.1145.2009.00347]
点击复制

藻菌生物膜胞外聚合物(EPS)与Al3+的配位作用机理()
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
15卷
期数:
2009年03期
页码:
347-350
栏目:
研究论文
出版日期:
2009-05-15

文章信息/Info

Title:
Characterization of the Complexation Between Al3+ and Extracellular Polymeric Substances Prepared from Alga-bacteria Biofilm
作者:
刘静张道勇潘响亮王立英
1中国科学院地球化学研究所环境地球化学国家重点实验室 贵阳 550002
2中国科学院新疆生态与地理研究所 乌鲁木齐 830011
3中国科学院研究生院 北京 100049
Author(s):
LIU JingZHANG DaoyongPAN XiangliangWANG Liying
1State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550002, China
2Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China
3Graduate University of the Chinese Academy of Sciences, Beijing 100049, China
关键词:
胞外聚合物Al3+三维荧光光谱荧光猝灭傅立叶转换红外光谱生物膜条件稳定常数
Keywords:
extracellular polymeric substance (EPS) Al3+ three-dimensional excitation emission matrix (3DEEM) fluorescence quenching Fourier transform infrared spectroscopy biofilm conditional stability constant
分类号:
X172 : Q936
DOI:
10.3724/SP.J.1145.2009.00347
文献标志码:
A
摘要:
摘 要 利用三维荧光光谱(3DEEM)和傅立叶转换红外光谱(FTIR)研究了藻菌生物膜EPS与Al3+的相互作用机理. 3DEEM结果表明,生物膜EPS含有3个荧光峰. 其中,峰A (Ex/Em=225~235 nm/300~330 nm)和峰B (Ex/Em=275~280 nm/325~330 nm)荧光较强,属类蛋白峰,峰C (Ex/Em=335 nm/432~434 nm)荧光较弱,属类腐殖酸峰. 峰A和峰B都能不同程度地被Al3+猝灭,它们的条件稳定常数(logK)分别为5.89和6.95. Al3+-EPS体系的峰A和峰B荧光强度明显受溶液pH值的影响. 在pH为2~4之间时,荧光强度随pH的增大而增大,在4~7之间随pH的增大而减小,在7~11之间随pH增大而增大. FTIR光谱图分析表明,Al3+主要与EPS中所含的—NH—、C==O等发生强的配位作用. 图6 参21
Abstract:
Three-dimensional excitation emission matrix fluorescence spectroscopy (3DEEM) and fourier transform infrared (FTIR) spectroscopy were used to study the extracellular polymeric substances (EPS) produced by alga-bacteria biofilm. Three excitation/emission (Ex/Em) fluorescence peaks at Ex/Em 225~235/300~330 nm (Peak A), 275~280/325~330 nm (Peak B), and 335/432~434 nm (Peak C) were identified in the 3DEEM, respectively. Peaks A and B were referred to as protein-like fluorescence and peak C as humic-like fluorescence. The fluorescence intensity at both peaks A and B decreased as Al3+ concentration increased. The values of logK (conditional stability constant) for peaks A and B were 5.89 and 6.95, respectively. It was also found that solution pH strongly affected the fluorescence intensity at peaks A and B for the Al3+-EPS complexation. The fluorescence intensity at peaks A and B increased consistently with solution pH increasing from 2 to 4, decreased with solution pH increasing from 4 to 7, and increased again with solution pH increasing from 7 to 11. FTIR analysis demonstrated that —NH— and C==O groups in EPS were responsible for binding with Al3+. Fig 6, Ref 21

参考文献/References:

1 Zhang DY (张道勇), Zhao YS (赵勇胜), Pan XL (潘响亮). The role of EPS in removing cadmium in sewage by algae-bacteria biofilm. Res Environ Sci (环境科学研究), 2004, 17 (5): 52~55
2 Zhang DY, Wang JL, Pan XL Cadmium sorption by EPSs produced by anaerobic sludge under sulfate-reducing conditions. J Hazardous Mat, 2006, 138: 589~593
3 Kuwae T, Hosokawa Y. Determination of abundance and biovolume of bacteria in sediments by dual staining with 49, 6-diamidino-2-phenylindole and acridine orange: Relationship to dispersion treatment and sediment characteristics. Appl & Environ Microbiol, 1999, 65 (8): 3407~3412
4 Liu Y, Lam MC, Fang HHP. Adsorption of heavy metals by EPS of activated sludge. Water Sci & Technol, 2001, 43 (6): 59~66
5 Guibaud G. Effect of pH on cadmium and lead binding by extracellular polymeric substances (EPS) extracted from environmental bacterial strains. Colloids & Surfaces B: Biointerfaces, 2008, 63: 48~54
6 Zhang BT (张波涛), Dong DM (董德明), Yang F (杨帆), Li Y (李鱼), Wu YH (吴雨华). Effect of ionic strength in solution on Pb2 + and Cd2+ adsorption to surface coatings. J Jilin Univ Earth Sci Ed (吉林大学学报地球科学版), 2004, 34 (4): 566~570
7 Dong DM (董德明), Ji L (纪亮),Hua XY (花修艺), Li Y (李鱼),Zheng N (郑娜). Studies on the characteristics of Co, Ni and Cu and adsorption to natural surface coatings. Chem J Chin Univ (高等学校化学学报), 2004, 25 (2): 247~251
8 Zhao JY, Nelson DJ. Fluorescence study of the interaction of Suwannee River fulvic acid with metal ions and Al3+-metal ion competition. J Inorganic Biochem, 2005, 99: 383~396
9 Kot A, NamiesNik J, The role of speciation in analytical chemistry. TrAC Trends Anal Chem, 2000, 19 (2/3), 69~79
10 Sheng GP, Zhang ML, Yu HQ. Characterization of adsorption properties of extracellular polymeric substances (EPS) extracted from sludge. Colloids & Surfaces B: Biointerfaces, 2008, 62: 83~90
11 Adav SS, Lee DJ. Extraction of extracellular polymeric substances from aerobic granule with compact interior structure. J Hazardous Mat, 2008, 154: 1120~1126
12 Guibaud G, Comte S. Comparison of the complexation potential of extracellular polymeric substances (EPS), extracted from activated sludges and produced by pure bacteria strains, for cadmium, lead and nickel. Chemosphere, 2005, 59: 629~638
13 Baker A. Fluorescence properties of some farm wastes: Implications for water quality monitoring. Water Res, 2002, 36: 189~195
14 Sheng GP, Yu HQ. Characterization of extracellular polymeric substances of aerobic and anaerobic sludge using three-dimensional and emission matrix fluorescence spectroscopy. Water Res, 2006, 40: 1233~1239
15 Wu FC, Midorikawa T, Tanoue E. Fluorescence properties of organic ligands for copper (II) in Lake Biwa and its rivers. Geochem J, 2001, 35: 333~346
16 Lamelas C, Benedetti M, Wilkinson KJ, Slaveykova VI. Characterization of H+ and Cd2+ binding properties of the bacterial exopolysaccharides. Chemosphere, 2006, 65: 1362~1370
17 Fu PQ (傅平青), Liu CQ (刘丛强), Wu FC (吴丰昌). Three-dimensional excitation emission matrix fluorescence spetroscopic charcterization of the complexation between mercury (1I) and dissolved organic matter. Environ Sci (环境科学), 2004, 25 (6): 140~144
18 Joaquim CG. Esteves da Silva. Fluorescence quenching of anthropogenie fulvie acids by Cu (Ⅱ), Fe (Ⅲ) and U. Talanta, 1998, 45: 1155~1165
19 Lu X, Jaffe R. Interaction between Hg (II) and natural dissolved organic matter:A fluorescence spectroscopy based study. Water Res, 200l, 35 (7): 1793~1803
20 Pan XL, Wang JL, Zhang DY. Biosorption of Pb (II) by Pleurotus ostreatus immobilized in calcium alginate gel. Proc Biochem, 2005, 40: 2799~2803
21 Wei GH (韦革宏), Li SJ (李砉俭), Xu WL (徐万里), Zhu ME (朱铭莪), Gong BS (龚报森), Characteristics of infrared spectra of pure cultures and exopolysaccharide from rhizobia. Chin J Appl Environ Biol (应用与环境生物学报), 1999, 5 (3): 31~34

相似文献/References:

[1]康福星,龙健,王倩,等.水环境中微生物胞外聚合物(EPS)对重金属和富营养元素的环境生物化学效应及其研究展望[J].应用与环境生物学报,2010,16(01):129.[doi:10.3724/SP.J.1145.2010.00129]
 KANG Fuxing,LONG Jian,WANG Qian,et al.Its Researching Prospect and Environmental & Biochemical Effect of Microbial Extracellular Polymeric Substances to Heavy Metals and Eutrophic Elements[J].Chinese Journal of Applied & Environmental Biology,2010,16(03):129.[doi:10.3724/SP.J.1145.2010.00129]
[2]王硕,于水利,徐巧,等.好氧颗粒污泥特性、应用及形成机理研究进展[J].应用与环境生物学报,2014,20(04):732.[doi:10.3724/SP.J.1145.2014.01009]
 WANG Shuo,YU Shuili,XU Qiao,et al.Characteristics, application and formation mechanisms of aerobic granular sludge: recent advances[J].Chinese Journal of Applied & Environmental Biology,2014,20(03):732.[doi:10.3724/SP.J.1145.2014.01009]
[3]李崇华 马贵党 尹 斌 焦小轩 季荣博 张春华 葛 滢**.莱茵衣藻胞外聚合物的提取和红外光谱表征*[J].应用与环境生物学报,2019,25(01):1.[doi:10.19675/j.cnki.1006-687x.2018.04020]

备注/Memo

备注/Memo:
*国家“863”项目(No. 2006AA06Z339),中国科学院“百人计划”项目和国家自然科学基金项目(Nos. 40673070,40872169)资助 Supported by the National High-tech Research and Development Program of China (863 Program, No. 2006AA06Z339), the Program of 100 Distinguished Young Scientists of the Chinese Academy of Sciences, and the National Natural Science Foundation of China (Nos. 40673070, 40872169)
**通讯作者 Corresponding author (E-mail: xiangliangpan@163.com)
更新日期/Last Update: 2009-07-03