|本期目录/Table of Contents|

[1]凌宏志,葛菁萍,魏薇,等.亚麻温水脱胶液中细菌菌群结构分析[J].应用与环境生物学报,2009,15(05):703-707.[doi:10.3724/SP.J.1145.2009.00703]
 LING Hongzhi,GE Jingping,WEI Wei & PING Wenxiang.Analysis of Bacterial Community in Water Retting of Flax[J].Chinese Journal of Applied & Environmental Biology,2009,15(05):703-707.[doi:10.3724/SP.J.1145.2009.00703]
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亚麻温水脱胶液中细菌菌群结构分析()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
15卷
期数:
2009年05期
页码:
703-707
栏目:
研究论文
出版日期:
2009-10-25

文章信息/Info

Title:
Analysis of Bacterial Community in Water Retting of Flax
作者:
凌宏志葛菁萍魏薇平文祥
(黑龙江大学生命科学学院,微生物黑龙江省高校重点实验室 哈尔滨 150080)
Author(s):
LING Hongzhi GE Jingping WEI Wei & PING Wenxiang
(Key Laboratory of Microbiology, College of Life Sciences, Heilongjiang University, Harbin 150080, China)
关键词:
亚麻细菌多样性变性梯度凝胶电泳(DGGE)温水脱胶
Keywords:
flax bacterial diversity denaturing gradient gel electrophoresis (DGGE) water retting
分类号:
Q938.15 : Q939.97
DOI:
10.3724/SP.J.1145.2009.00703
文献标志码:
A
摘要:
为了探讨实验室条件下模拟的工厂亚麻温水脱胶液中细菌菌群结构,采用纯培养技术和PCR-DGGE技术(Denaturing gradient gel electrophoresis)对细菌菌群结构进行了研究. 用纯培养方法分离获得9类菌落,其中假单胞菌属(Pseudomonas)在有氧培养条件下总是处于优势. 梭菌属(Clostridium)在厌氧培养过程中总是处于优势. 微球菌属(Micrococcus)和葡萄球菌属(Staphylococcus)只有亚麻脱胶初期才有发现. PCR-DGGE指纹图谱显示,亚麻温水脱胶过程中条带数量较少且没有明显种群群落结构演替过程. 通过对不同时期沤麻液中16S rDNAV3片段PCR产物d、e两个DGGE条带进行分子克隆、序列测定和Blast分析,发现e条带包含的16S rDNAV3片段除e_35外均属于假单胞菌属. d条带包含着较多不同的16S rDNAV3片段,其中有传统方法没有分离到的泛菌属(Pantoea)细菌、一些NCBI未收录的序列及一些非可培养微生物序列. 纯培养技术和PCR-DGGE技术的共同使用,可以更全面准确地提供细菌多样性方面的信息. 图4 参15
Abstract:
The bacterial community structure in water retting systems of Bei’an flax in Heilongjiang, China was studied by using denaturing gradient gel electrophoresis (DGGE) and culture-dependent method, respectively. The results obtained from the culture-independent method showed that Pseudomonas and Clostridium were found the dominant genera using aerobic and anaerobic cultivation in water retting, respectively. Micrococcus and Staphylococcus were only observed within initial 16 h. The profile of DGGE fingerprints in water retting of different periods revealed that the bacterial groups group were relatively fewer. As a result, two major bands of 16S rDNA genes fragments from DGGE profiles were further eluted from gel, prior to be reamplified and sequenced. The sequences of these fragments were compared with the database of GenBank (NCBI). 9 genera were identified from the water retting with the culture-dependent method. Compared with the database of GenBank, the results displayed that 11 sequences of band d shared 92%~99% homology of the known sequences (Pantoea, Bacillus, unidentified bacterium and new bacteria), while the 12 sequences of band e shared 96%~100% homology of the known sequences (Pseudomonas). This suggests that a combination of molecular and culture-dependent methods can be used to analyze and monitor the community structure of water retting effectively, and will give us more information of microorganism community structure. Fig 4, Ref 15

参考文献/References:

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备注/Memo

备注/Memo:
黑龙江省自然科学基金项目(No. C200505),黑龙江省博士后启动基金项目(No. LBH-Q05113),黑龙江省人事厅留学回国择优资助项目,黑龙江省教育厅振兴老工业基地成果转化项目(No. 1152GZH03)和哈尔滨市科技攻关项目(No. 2007AA6CN032)资助 Supported by the Natural Science Foundation of Heilongjiang, China (No. C200505), the Post Doctorial Startup Fund of Heilongjiang, China (No. LBH-Q05113 ), the Advanced Program of Department of Personnel of Heilongjiang, China, the Education Department of Heilongjiang, China (No.1152GZH03) and the Science and Technology Foundation of Harbin, China (No. 2007AA6CN032)
更新日期/Last Update: 2009-10-26