|本期目录/Table of Contents|

[1]何力,郝勃,谢从新,等.草鱼肠道纤维素酶产生菌主要种类的分离与鉴定[J].应用与环境生物学报,2009,15(03):414-418.[doi:10.3724/SP.J.1145.2009.00414]
 HE Li,HAO Bo,XIE Congxin,et al.Isolation and Identification of Major Cellulase-producing Fungi in Intestines of Grass Carp[J].Chinese Journal of Applied & Environmental Biology,2009,15(03):414-418.[doi:10.3724/SP.J.1145.2009.00414]
点击复制

草鱼肠道纤维素酶产生菌主要种类的分离与鉴定()
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
15卷
期数:
2009年03期
页码:
414-418
栏目:
生物质能源专辑
出版日期:
2009-05-15

文章信息/Info

Title:
Isolation and Identification of Major Cellulase-producing Fungi in Intestines of Grass Carp
作者:
何力郝勃谢从新罗晓松张征朱祥云
1中国水产科学研究院长江水产研究所 荆州 434000
2华中农业大学水产学院 武汉 430070
Author(s):
HE LiHAO BoXIE CongxinLUO XiaosongZHANG ZhengZHU Xiangyun
1Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Jingzhou 434000, Hubei, China
2College of Aquaculture, Huazhong Agricultural University, Wuhan 430070, China
关键词:
草鱼肠道纤维素酶产生菌分离鉴定
Keywords:
grass carp intestine cellulase producing strain isolation identification
分类号:
Q959.468.06
DOI:
10.3724/SP.J.1145.2009.00414
文献标志码:
A
摘要:
摘 要 从我国特有的草鱼肠道内分离到纤维素酶产生菌,其中4株具有较高的纤维素分解能力,测量了其羧甲基纤维素酶(CMCase)、棉花酶和滤纸酶(FPase)活性,并进行了种类鉴定. 结果表明,草鱼肠道具有一定的纤维素分解能力,可能来自于肠道细菌所产生的外源性纤维素酶的协同作用;草鱼肠道内纤维素分解菌具有较高酶活性;不同菌株所产生的相同纤维素酶的活性存在差异;其中,X7的CMCase活性最高,达0.83 U/mL,X5的棉花酶活性最高,达0.87 U/mL,X8的FPase活性最高,达0.54 U/mL. 4种菌株的菌落形态有差异,经16S rRNA基因鉴定,X5、X6、X7为枯草芽胞杆菌(Bacillus subtilis),X8为阿氏肠杆菌( Enterobacter asburiae). 图6 表3 参19
Abstract:
Abstract Cellulase producing strains were isolated from the intestines of grass carp which is endemic to China, and 4 of them were found with high cellulase activity. The activities of carboxymethyl cellulase (CMCase), cotton lyase and filter paper enzyme (FPase) were measured and the species were identified. The results showed that the intestines of grass carp had the ability to decompose cellulase, which might be the synergistic effect of exogenous cellulase produced in the intestines. The cellulase producing strains in grass carp intestines possessed high enzyme activities, which were different in the strains. CMCase in X7 had the higest activity with the value of 0.83 U/mL, while X5 and X8 had the highest cotton lyase and FPase with the values of 0.87 and 0.54 U/mL, respectively. The colony morphology was different among the 4 strains. 16S rRNA genotype identification indicated that X5, X6 and X7 belonged to Bacillus subtilis and X8 to Enterobacter asburiae. Fig 6, Tab 3, Ref 19

参考文献/References:

1 Xiao CL (肖春玲), Xu CX (徐常新). Application study on microbialcellulase. J Microbiol (微生物杂志), 2002, 22 (2): 33~35
2 Wu XR (吴显荣), Mu XM (穆小民). 纤维素酶分子生物学研究进展及趋向. 生物工程进展, 1994, 14 (4): 25~27
3 Hungate RE. The rumen microbial system. Annu Rev Ecol & Syst, 1975, 6: 39~66
4 BjorndalK A. Cellulose digestion and volatile fatty acid production in green turtle Cheloniamydas. Comp Biochem & Physiol, 1979, 63A: 127~133
5 Trust TJ, Sparrow RAH. The bacterial flora in the alimentary tract of freshwater salmonid fishes. Can J Microbiol, 1974, 20: 1219~1228
6 Teather RM, Wood PJ. Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. Appl Environ Microbiol, 1982, 43 (4): 777~780
7 Shen XL (沈雪亮), Xia LM (夏黎明). Studies on screening of cellulose producing bacteria and enzymatic characteristics. Chem & Ind For Prod (林产化学与工业), 2002 (1): 47~51
8 Yan MH (严民宏),Wang EL (王二力), Zhou YC (周元聪), Chen YC (陈远聪), Tao ZJ (陶宗晋). Studies on ultrafiltration flux rate of glucoamylase. J East China Inst Chem Technol (华东化工学院学报), 1991 (2): 82~86
9 T.K. Ghose, Measurements of cellulase activities, Pure Appl Chem 1987, 59: 257~268
10 祖若夫, 温宝龙. 微生物实验教程. 上海: 复旦大学出版社, 1993. 58~60
11 东秀珠, 材妙英. 常见细菌系统鉴定手册. 北京: 科学出版社, 2001. 253~272
12 Marmur JA. Procedure for the isolation of deoxyribonucleic acid from microorganism. J Mol Biol, 1969, 3: 208~218
13 张龙翔. 生化实验方法和技术. 北京: 高等教育出版社, 1997. 1~3
14 Horikoshi K, Nakao M, Kurono Y, Sashihara N. Cellulases of an alkalophilic Bacillus strain isolated from soil. Can J Microbiol, 1984, 30: 774~779
15 Liu F (刘飞), Zhang FQ (张凤琴), Zhao QZ (赵强忠), Zhao MM (赵谋明). Effect of adding nutrients and Bacillus subalis on purification of aquaculture wastewater. J Agro-Environ Sci (农业环境科学报), 2007, 26 (4): 1282~1286
16 Chang WS (常维山), Zhang CY (张春阳), Niu ZX (牛钟相), Zhu LX (朱立贤), Xie YM (谢幼梅). Selection, identification and application of probiotic bacterium which produces high activity proteimase. Chin J Anim Sci (中国畜牧杂志), 2003, 39 (3): 11~12
17 Jin ZX (金志雄), Wu WQ (吴文琴), Zhang Z (张珍). Study on the disintegrating starch ability of B. subtilis. Chin J Microecol (中国微生态学杂志), 2004, 16 (3): 142~143
18 Qin YX (覃映雪), Wang XL (王晓林), Yan QP (鄢庆枇), Wang B (王贇), Ye DF (叶冬凤). Study on the intestinal microflora of Epinephelus awoara. Mar Fish Res (海洋水产研究), 2007, 28 (5): 18~23
19 Beauchemin KA, Rode LM, Sewah VJH. Fibrolytic enzymes increase fiber digestibility and growth rate of steers fed dry forage. J Anim Sci, 1995, 75: 641~644

相似文献/References:

[1]吴初新,刘毅,马梅生,等.热休克、Poly I: C上调草鱼GRP78基因表达[J].应用与环境生物学报,2009,15(06):814.[doi:10.3724/SP.J.1145.2009.00814]
 WU Chuxin,LIU Yi,MA Meisheng,et al.Up-regulation of Grass Carp GRP78 Gene Expression under Heat Shock and Poly I:C Stress[J].Chinese Journal of Applied & Environmental Biology,2009,15(03):814.[doi:10.3724/SP.J.1145.2009.00814]

备注/Memo

备注/Memo:
*国家科技支撑计划项目(No. 2006BAD01A12)和中国水产科学院淡水生态与健康养殖重点实验室开放课题项目(No. FEA-2006108)资助 Supported by the Key Sci & Tech Project of the 11th Five-year Plan of China (No. 2006BAD01A12) and the Exoteric Project of Key Laboratory of Freshwater Ecology and Healthy Aquaculture, CAFS (No. FEA-2006108)
**通讯作者 Corresponding author (E-mail: xiecongxin@sohu.com)
更新日期/Last Update: 2009-07-03