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 LI Cui,CHAI Baofeng.Genetic Conservation of Pinus tabulaeformis Population Implicated by Molecular Markers[J].Chinese Journal of Applied & Environmental Biology,2009,15(04):459-463.[doi:10.3724/SP.J.1145.2009.00459]





Genetic Conservation of Pinus tabulaeformis Population Implicated by Molecular Markers
1山西财经大学环境经济学院,2山西大学生物技术研究所 太原 030006
LI CuiCHAI Baofeng
1School of Environment Economics, Shanxi University of Finance and Economics, Taiyuan 030006, China
2Institute of Biotechnology, Shanxi University, Taiyuan 030006, China
Pinus tabulaeformismolecular markergenetic diversitypopulation structuregenetic conservation
利用分子标记技术分析了山西5个天然油松种群140个个体的遗传特征,比较了不同种群的多态位点显性频率、遗传多样性指数、基因流和分化系数. 筛选的5个ISSR引物和5个RAPD引物共扩增到位点68个,其中多态位点50. 遗传参数的统计分析显示,多态位点的显性频率、遗传多样性指数、分化系数分别介于0.000 0~1.000 0、0.020 0~0.500 0和0.003 7~0.615 6之间,表明油松种群遗传多样性的主要来源是等位基因频率的不同和部分位点较大程度的遗传分化. 根据油松的种群遗传学特征,本研究认为只有加强天然油松林的生境保护,使其不产生片断化,才能使油松的遗传资源得到有效保护. 图2 表4 参16
Pinus tabulaeformis widely distributions in China from northeast to southwest, and plays an important role in natural forests and artificial plantations. Its genetic characteristics of DNA in 140 samples from 5 populations in Shanxi were investigated by using molecular technique. A total of 68 reproducible bands were identified using 5 RAPD primers and 5 ISSR primers, in which 50 bands were polymorphicl loci. The statistic analysis of the genetic parameters indicated that gene frequency of dominants, genetic diversity index and the relative magnitude of differentiation among populations were 0.000 0~1.000 0, 0.020 0~0.500 0 and 0.003 7~0.615 6, respectively, suggesting that the genetic diversity of P. tabulaeformis populations in Shanxi mainly lies on different allelic frequencies and on some high level genetic differentiating locus. Based on the characteristics of P. tabulaeformis populations in Shanxi, we suggested that only by protecting it’s territory and keeping them integration, could the genetic resource of pine be well conserved. Fig 2, Tab 4, Ref 16


1 徐化成主编. 油松地理变异和种源选择. 北京: 中国林业出版社, 1991. 1~20
2 Hamrick JL, Godt MJW. Conservation genetics ofendemic plant species. In: Avise JC, Hamrick JL eds. Conservation Genetics: Case Histories from Nature. New York, USA: Chapman and Hall, 1996. 281~304
3 Hogbin PM, Peakall R. Evaluation ofthe contribution ofgenetic research to the management ofthe endangered plant Zieria prostrate. Conserv Biol, 1999, 13: 514~5224 Zhang DM (张冬梅), Yang Y (杨娅), Shen XH (沈熙环), Ru GX (茹广欣). Selection of primers and establishment of SSR-PCR reaction system on Pinus tabulaeformis Carr. J Beijing For Univ (北京林业大学学报), 2007, 29 (2): 13~17
5 Liu ZL (刘占林), Yang X (杨雪). Genetic diversity and differentiation of five pines using cpSSR and AFLP markers. Acta Bot Bor-Occid Sin (西北植物学报), 2007,27 (12): 2385~2392
6 Chen K, Abbott RJ, Milne RI, Tian XM, Liu J. Phylogeography of Pinus tabulaeformis Carr. (Pinaceae), a dominant species of coniferous forest in northern China. Mol Ecol, 2008, 17: 4276~4288
7 Li C (李毳), Chai BF (柴宝峰), Wang MB(王孟本). Genetic diversity of Pinus tabulaeformis populations in North China. Bull Bot Res (植物研究), 2006, 26 (1): 98~102
8 Li C (李毳), Chai BF (柴宝峰), Liang AH (梁爱华), Wang MB(王孟本).  Comparison of extracting reagents of prolamin from Pinus tabulaeformis seed. Chin J Appl Environ Biol (应用与环境生物学报), 2006, 12 (1): 25~127
9 Zhang WH (张文辉), Liu GB (刘国彬). Review on vegetation restoration in Loess Plateau. Sci Silv Sin (林业科学), 2007, 43 (1): 102~106
10 Mehes MS, Nkongolo KK, Michael P. Genetic analysis of Pinus strobus and Pinus monticola populations from Canada using ISSR and RAPD markers: Development of genome-specific SCAR markers. Plant Syst & Evol, 2007, 267: 47~63
11 Zhao CF (赵春芳), Chen GJ (陈国娟), Wang YH (王芋华), Korpelqinen H, Li CY (李春阳). Genetic variation of Hippophae rhamnoides populations at different altitudes in the Wolong Nature Reserve based on RAPDs. Chin J Appl Environ Biol (应用与环境生物学报), 2007, 13 (6): 753~758
12 Doyle JJ, Doyle JL. A rapid DNA isolation procedure for small quantities of fresh leaf material. Phytochem Bull, 1987, 19:11~15
13 Yeh FC, Boyle TJB. Population genetic analysis of co-dominant and dominant markers and quantitative traits. Belg J Bot, 1997, 129: 15714 Schemske DW, Husband BC, Ruckelsmaus MH, Goodwillie C, Parker IM, Bishop JG. Evaluating approaches to the conservation of rare and endangered plants. Ecology, 1994, 75: 584~606
15 Qu RZ (曲若竹), Hou L (侯林), Lü HL (吕红丽), Li HY (李海燕). The gene flow of population genetic structure. Genetics (遗传), 2004, 26 (3): 77~382
16 Merrell DJ. Ecological Genetics . London: Longman, 1981


 YAN Naihong,et al..Conversion of RAPD marker to SCAR marker of wheat cereal rootknot nematoderesistant gene Rknmnl[J].Chinese Journal of Applied & Environmental Biology,2003,9(04):250.
 MA Xinrong,et al..Application of Plant Biotechnology in Ryegrass Genetic Improvement[J].Chinese Journal of Applied & Environmental Biology,2007,13(04):881.
 ZHANG Yun,ZHAO Yazhou,ZHANG Chunyu & ZHAO Xiuhai.Structure and Spatial Distribution of Pinus tabulaeformis Population in the Songshan Nature Reserve, Beijing, China[J].Chinese Journal of Applied & Environmental Biology,2009,15(04):175.[doi:10.3724/SP.J.1145.2009.00175]
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 HE Fan,WANG Dexiang,ZHANG Songzhi,et al.Reserves of Litter and Woody Debris of Two Main Forests in the Xiaolong Mountains, Gansu, China[J].Chinese Journal of Applied & Environmental Biology,2011,17(04):46.[doi:10.3724/SP.J.1145.2011.00046]
 JIANG Qingbiao,GAO Lushuang,WANG Xiaoming,et al.Correlations Between Meteorological Factors and Tree-ring Width Index of Chinese Pine (Pinus tubulaeformis) in Hunshandake Sandy Area, China[J].Chinese Journal of Applied & Environmental Biology,2012,18(04):405.[doi:10.3724/SP.J.1145.2012.00405]
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 LI Cui,CHAI Baofeng.Characteristics and Systematic Significance of Chloroplast DNA Sequences of Pinus tabulaeformis[J].Chinese Journal of Applied & Environmental Biology,2010,16(04):143.[doi:10.3724/SP.J.1145.2010.00143]


山西省回国留学人员科研基金(No. 20 0846)和国家自然科学基金(No. 30770294)资助
更新日期/Last Update: 2009-08-26