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 WANG Qilin,NIU Xiangli,LIU Yongsheng.Post-transcriptional Processing of Two Functions of Unknown Transcription Factor Genes in Zea mays[J].Chinese Journal of Applied & Environmental Biology,2009,15(04):483-487.[doi:10.3724/SP.J.1145.2009.00483]





Post-transcriptional Processing of Two Functions of Unknown Transcription Factor Genes in Zea mays
四川大学生命科学学院生物资源与生态环境教育部重点实验室 成都 610064
WANG QilinNIU XiangliLIU Yongsheng
Key Laboratory for Bio-resource and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, China
Zea mayspost-transcriptional processingtranscription factorshort-direct repeat
Q78 : S513.01
ZmBH、ZmWD是玉米中分别具有转录因子bHLH和WD重复蛋白结构的两个功能未知基因,在不同组织中及各种胁迫诱导条件下,这两个基因均发生不同程度的剪接,产生大小各异的转录本. 序列分析发现其剪接方式与经典的mRNA剪接不同,即剪接位点的选择不符合GT-AG或AT-AC规则,而是在剪接位点具有短正向重复序列SDR (Short-direct repeats). ZmBH的转录后加工导致序列发生移码而提前终止,而ZmWD则缺失了大部分结构域序列,经由这种方式产生的转录后加工产物可影响蛋白编码,因而可能是对这两个基因功能进行调控的一种方式. 图6 表1 参18
ZmBH and ZmWD, containing bHLH or WD40-repeat demain, are unknown function transcription factor-like genes in maize. Under different treatments, various transcripts were determined in the two genes. Sequence analyses demonstrated that unlike the classic splicing processes, the splicing sites were neither GT-AG nor AT-AC, but short-direct repeats (SDRs), which present surrounding splicing junctions in the individual splicing events. The unusual post-transcriptional processing led to a premature termination by introducing stop codon in ZmBH, and a deletion of functional domain in ZmWD. Therefore, the majority of the detected transcripts resulting from SDR-mediated post-transcriptional processing might have no capacity to encode functional proteins, suggesting a regulation mechanism of ZmBH and ZmWD. Fig 6, Tab 1, Ref 18


1 Cech TR. RNA splicing: Three themes with variations. Cell, 1983, 34: 713~716
2 Bailey PC, Martin C, Toledo-Ortiz G. Update on the basic helix-loop-helix transcription factor gene family in Arabidopsis thaliana. Plant Cell, 2003, 15: 2497~2502
3 Jurica MS, Moore MJ. Pre-mRNA splicing: Awash in a sea of proteins. Mol Cell, 2003, 12: 5~14
4 Danielle M, Jennifer N. Three redundant brassinosteroid, early response genes encode putative bHLH transcription factors required for normal growth. Genetics, 2002, 162: 1445~1456
5 Ni M, Tepperman JM. Quail P H. PIF3, a phytochrome-interacting factor necessary for normal photoinduced signal transduction, is a novel basic helix-loop-helix protein. Cell, 1998, 95: 657~667
6 Heisler MG, Atkinson A, Bylstra YH, Walsh R, Smyth DR. SPATULA, a gene that controls development of carpel margin tissues in Arabidopsis, encodes a bHLH protein. Development, 2001, 128: 1089~1098
7 Gonzalez E, Solano R, Rubio V, Leyva A, Paz-Ares J. Phoshate Transporter Traffic Facilitator1 is a plant-specific SEC12-related protein that enables the endoplasmic reticulum exit of a high-affinity phosphate transporter in Arabidopsis. Plant Cell, 2005, 17: 3500~3512
8 d’Enfert C, Gensse M, Gaillardin C. Fission yeast and a plant have functional homologues of the Sar1 and Sec12 proteins involved in ER to Golgi traffic in budding yeast. EMBO J. 1992, 11: 4205~4211
9 Levy B, McKie JM, Duncan AM, Hirschhorn K, Bancroft C. Cloning and characterization of human PREB: A gene that maps to a genomic region associated with trisomy 2p syndrome. Mamm Genome, 2000, 11: 675~681
10 Taylor Clelland CL, Craciun L, Bancroft C, Lufkin T. Mapping and developmental expression analysis of the WD-repeat gene Preb. Genomics, 2000, 63: 391~399
11 Fliss MS, Hinkle PM, Bancroft C. Expression cloning and characterization of PREB (prolactin regulatory element binding), a novel WD motif DNA-binding protein with a capacity to regulate prolactin promoter activity. Mol Endocrinol, 1999, 13: 644~657
12 Blanc V, Davidson NO. C-to-U RNA Editing: Mechanisms leading to genetic diversity. J Biol Chem. 2003, 278: 1395~1398
13 Goodrich J, Carpenter R, Coen ES. A common gene regulates pigmentation pattern in diverse plant species. Cell, 1992, 68: 955~964
14 Smith TF, Gaitatzes C, Saxena K. The WD repeat: a common architecture for diverse functions. Trends Biochem Sci, 1999, 24: 181~185
15 Rossi V, Varotto S, Locatelli S. The maize WD-repeat gene ZmRbAp1 encodes a member of the MSI/RbAp sub-family and is differentially expressed during endosperm development. Mol Genet Genomics, 2001, 265: 576~584
16 Niu XL, Zheng WJ, Lu B-R, Ren GJ, Huang WZ, Wang SH, Liu JL,Tang ZZ, Luo D, Wang YG, Liu YS. An unusual posttranscriptional processing in two betaine aldehyde dehydrogenase (BADH)loci of cereal crops directed by short-direct repeats in response to stress conditions. Plant Physiol, 2007, 143: 1929~1942
17 Luo D, Niu XL, Wang YG, Zheng WJ, Chang LJ, Wang QL, Wei X,Yu G, Lu BR, Liu YS. Functional defect at rice choline monooxygenase locus from an unusual post-transcriptional processing associated with the sequence elements of short direct repeats. New Phytol, 2007, 175: 439~447
18 Fan J, Niu XL, Wang YG, Ren GJ, Yang Y , Lu BR, Liu YS. Short, direct repeats (SDRs)-mediated post-transcriptional processing of a transcription factor gene OsVP1 in rice (Oryza sativa). J Exp Bot, 2007, 58: 3811~3817


 Geng Ruishuang,Miao Shuhua,He Liming.MAIZE SEED PROTEIN MUTANTS RESISTANT TO LYSINE PLUS THREONINE[J].Chinese Journal of Applied & Environmental Biology,1995,1(04):1.
 Song Songquan,Jian Weijun,Fu Jiarui.EFFECT OF Cd2+ ON SEED VIGOR OF ZEA MAYS L. AND ANTAGONISM OF Ca2+ TO Cd2+[J].Chinese Journal of Applied & Environmental Biology,1997,3(04):1.
 ZHANG Aiqun,HE Liyuan,ZHAO Huie,et al.Effects of Phosphorus Deficiency and Al toxicity on Biological and Nutrient Characteristics of Maize Seedlings[J].Chinese Journal of Applied & Environmental Biology,2009,15(04):48.[doi:10.3724/SP.J.1145.2009.00048]
 ZHANG Yiwei,DI Xiaoyan,WU Dongmei,et al.Effects of Combined Pollution of SO2 and PAHs on Seed Germination and Growth of Maize in Shanxi Industrial and Mining Area[J].Chinese Journal of Applied & Environmental Biology,2013,19(04):134.[doi:10.3724/SP.J.1145.2013.00134]
 ZHOU Lunli,XIE Guangning,ZHANG Zhenbao,et al.Responses of two maize inbred lines of different aluminium tolerance to Al-induced oxidative stress[J].Chinese Journal of Applied & Environmental Biology,2014,20(04):669.[doi:10.3724/SP.J.1145.2014.04013]
 YANG Shanshan,WANG Qian,HU Hongling,et al.Allelopathy of Cinnamomum septentrionale leaf litter on maize growth and relieving effect of fertilization[J].Chinese Journal of Applied & Environmental Biology,2015,21(04):770.[doi:10.3724/SP.J.1145.2015.04033]
 LIAO Yuhua,HU Hongling**,CHEN Yufeng,et al.Heterologous overexpression of Populus euphratica CPD improved tobacco resistance to salt, high nitrogen, and drought stress[J].Chinese Journal of Applied & Environmental Biology,2017,23(04):635.[doi:10.3724/SP.J.1145.2016.09028]
 LI Shanjia,WANG Junqiang,et al.Effect of different base fertilizer treatments on maize soil enzyme activity and glomalin-related protein[J].Chinese Journal of Applied & Environmental Biology,2017,23(04):357.[doi:10.3724/SP.J.1145.2016.04045]
 YUAN Xianfu,SUN Yuhan,ZHU Chengzhi,et al.Rotation combined with bio-organic fertilizer application to promote banana growth[J].Chinese Journal of Applied & Environmental Biology,2018,24(04):60.[doi:10.19675/j.cnki.1006-687x.2017.03022]
 YANG Shanshan,WANG Qian,HU Tingxing,et al.Physiological responses to allelopathy of decomposing Cinnamomum septentrionale leaf litter of three crops (corn, cucumber, and cowpea)[J].Chinese Journal of Applied & Environmental Biology,2018,24(04):292.[doi:10.19675/j.cnki.1006-687x.2017.05016]


国家自然科学基金项目(No. 30770466)资助
更新日期/Last Update: 2009-08-26