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[1]胡杰,李大平,陶勇,等.不同C/N值下亚硝酸盐氧化菌和异养菌混合体系的微生物多样性[英 文][J].应用与环境生物学报,2009,15(03):351-355.[doi:10.3724/SP.J.1145.2009.00351]
 HU Jie,LI Daping,TAO Yong,et al.Microbial Diversity of Nitrite-oxidizing and Heterotrophic Bacterial Communities under Different C/N Ratios[J].Chinese Journal of Applied & Environmental Biology,2009,15(03):351-355.[doi:10.3724/SP.J.1145.2009.00351]
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不同C/N值下亚硝酸盐氧化菌和异养菌混合体系的微生物多样性[英 文]()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
15卷
期数:
2009年03期
页码:
351-355
栏目:
研究论文
出版日期:
2009-05-15

文章信息/Info

Title:
Microbial Diversity of Nitrite-oxidizing and Heterotrophic Bacterial Communities under Different C/N Ratios
作者:
胡杰李大平陶勇张金莲钟琦何晓红王晓梅
中国科学院成都生物所应用与环境微生物中心 成都 610041
Author(s):
HU JieLI DapingTAO YongZHANG JinlianZHONG QiHE XiaohongWANG Xiaomei
Center for Applied and Environmental Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China
关键词:
微生物多样性PCR-限制性片段长度多态性(RFLP)亚硝酸盐氧化菌(NOB)C/N比
Keywords:
microbial diversity PCR-restriction fragment length polymorphism (RFLP) nitrite-oxidizing bacteria (NOB) C/N ratio
分类号:
Q938.15
DOI:
10.3724/SP.J.1145.2009.00351
文献标志码:
A
摘要:
摘 要 采用PCR-RFLP技术研究了不同C/N比下亚硝酸盐氧化菌及异养菌混合体系的微生物多样性,并探讨了微生物菌群结构与其功能(硝化性能)的关系. C/N=0时,混合体系主要由自养菌和寡营养菌(85.1%)组成,包括亚硝酸盐氧化菌(NOB)、拟杆菌门、α-变形菌纲、浮霉菌门和绿色非硫细菌中的一些菌株. C/N=0.44时,混合体系中的自养菌减少,异养菌(主要是γ-变形菌纲的成员)大量出现. C/N=8.82时,γ-变形菌纲的菌株尤其是反硝化菌Pseudomonas sp.占主导(93.8%). 与此同时,随着C/N升高,该混合体系的硝化性能也由专一的亚硝酸盐氧化过程转变为同时硝化反硝化过程. 微生物菌群结构的转变较好地解释了其硝化性能的改变. 本研究揭示了微生物菌群结构与其功能的内在联系,同时表明PCR-RFLP技术与化学分析相结合是研究微生物菌群结构与功能的有力工具. 图3 表2 参13
Abstract:
Abstract The microbial diversity of nitrite-oxidizing and heterotrophic bacterial communities at three C/N ratios of feed solutions was experimentally investigated using PCR- restriction fragment length polymorphism (RFLP) technique and correlated with nitrification performances of the mixture. At C/N = 0, the dominant populations were mainly autotrophic and oligotrophic bacteria (85.1%), including nitrite-oxidizing bacteria (NOB), and some strains of Bacteroidetes, Alphaproteobacteria, Actinobacteria, and green nonsulfur bacteria. At C/N = 0.44, autotrophic bacteria reduced, while heterotrophic bacteria (members of Gammaproteobacteria) increased. At C/N =8.82, members of Gammaproteobacteria dominated (93.8%), especially denitrifiers, i.e., Pseudomonas sp. And on the other side, a striking shift of nitrification performance occurred from a normal nitrite oxidation process to a simultaneous nitrification and denitrification process along with increasing C/N. The nitrification performances at the three C/N ratios could be well explained by the obtained microbial diversities. This study demonstrated the linkage between the functional performance (nitrification performance) and the microbial diversity, suggesting that PCR- RFLP technique together with functional parameter analysis has potential as a tool for relating functional variety to bacterial community shifts. Fig 3, Tab 2, Ref 13

参考文献/References:

1 Sharma B, Ahlert RC. Nitrification and nitrogen removal. Water Res, 1977, 11: 897~925
2 Ohashi A, de Silva DG, Mobarry B, Manem JA, Stahl DA, Rittmann BE. Influence of substrate C/N ratio on the structure of multi-species biofilms consisting of nitrifiers and heterotrophs. Wat Sci Tech, 1995, 32: 75~84
3 Okabe S, Hiratia K, Ozawa Y, Watanabe Y. Spatial microbial distributions of nitrifiers and heterotrophs in mixed-population biofilms. Biotechnol Bioeng, 1996, 50: 24~35
4 Michaud L, Blancheton JP, Brunl V, Piedrahita R. Effect of particulate organic carbon on heterotrophic bacterial populations and nitrification efficiency in biological filters. Aquacult Eng, 2006, 34: 224~233
5 Urakawa H, Kita-Tsukamoto K, Ohwada K. 16S rDNA genotyping using PCR/RFLP (restriction fragment length polymorphism) analysis among the family Vibrionaceae. FEMS Microbiol Lett, 1997, 152: 125~132
6 Ramirez-Moreno S, Martinez-Alonso MR, Mendez-Alvarez S, Esteve I, Gaju N. Seasonal population changes in the restriction fragment length polymorphism (RFLP) patterns from PCR-amplified 16S rRNA genes of predominant ribotypes in microbial mat samples from the Ebro Delta (Spain). Curr Microbiol, 2003, 46: 190~198
7 Miyoshi T, Iwatsuki T, Naganuma T. Phylogenetic characterization of 16S rRNA gene clones from deep-groundwater microorganisms that pass through 0.2-micrometer-pore-size filters. Appl Environ Microbiol, 2005, 71: 1084~1088
8 Tamura K, Dudley J, Nei M, Kumar S. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol & Evol, 2007, 24: 1596~1599
9 Hill TCJ, Walsh KA, Harris JA, Moffett BF. Using ecological diversity measures with bacterial communities. FEMS Microbiol Ecol, 2003, 43: 1~11
10 Blaszczyk M. Effect of various sources of organic carbon and high nitrite and nitrate concentrations on the selection of denitrifying bacteria. II. Continuous cultures in packed bed reactors. Acta Microbiol Pol, 1983, 32: 65~71
11 Ping L, De-Li L, Nahimana L, Chen SL, Yang X, Zhao L. High nitrogen removal from wastewater with several new aerobic bacteria isolated from diverse ecosystems. J Environ Sci (China), 2006, 18: 525~529
12 Song L, Wang H, Jiang J, Gao JS, Shi HC. Isolation, idetification of 1,2,4-trichlorobenzene-degrading strain Pseudomonas nitroreducens J5-1 and cloning of chlorocatechol 1,2-dioxygenase gene. Environ Sci, 2007, 28: 1878~1881
13 Braker G, Zhou J, Wu L, Devol AH, Tiedje JM. Nitrite reductase genes (nirK and nirS) as functional markers to investigate diversity of denitrifying bacteria in pacific northwest marine sediment communities. Appl Environ Microbiol, 2000, 66: 2096~2104

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备注/Memo

备注/Memo:
?Supported by the National High-tech Research and Development Program (“863” Program) of China (Nos. 20062AA05Z103,2007AA06Z324)
**Corresponding author (E-mail: lidp@cib.ac.cn)
更新日期/Last Update: 2009-07-03