|本期目录/Table of Contents|

[1]孙丰慧,李安明.耐碱性木聚糖酶产生菌的筛选及发酵条件研究[J].应用与环境生物学报,2008,14(03):436-439.
 SUN Fenghui,et al..Screening of an AlkaliTolerant Xylanase Producing Strain and Its Optimization of Enzyme Production[J].Chinese Journal of Applied & Environmental Biology,2008,14(03):436-439.
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耐碱性木聚糖酶产生菌的筛选及发酵条件研究()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
14卷
期数:
2008年03期
页码:
436-439
栏目:
综述
出版日期:
2008-03-25

文章信息/Info

Title:
Screening of an AlkaliTolerant Xylanase Producing Strain and Its Optimization of Enzyme Production
作者:
孙丰慧李安明
Author(s):
SUN Fenghui et al.
(1Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China) (2Graduate University of Chinese Academy of Sciences, Beijing 100049, China)
关键词:
关键词 耐碱性木聚糖酶菌株筛选发酵条件纤维菌属酶活力
Keywords:
Keywords alkalitolerant xylanase strain screening fermentation condition Cellulosimicrobium enzyme activity
Abstract:
Abstract Using the Congored staining method, an alkalitolerant xylanase producing strain X2414 was screened from alkaline soil. It was subsequently identified as Cellulosimicrobium sp. based on its morphological characters and 16S rDNA sequence. The result of the optimized fermentation conditions showed that the maximum xylanase activity (2 204 u/mL) was achieved in the medium containing 60 g/L wheat bran, 10 g/L peptone, 7 g/L K2HPO4, with inoculum size 5% and 200 r/min at pH 8.5 under 37 ℃ for 108 h. The optimal temperature of the xylanase was 60 ℃. The xylanase was found with good alkali tolerance and high activity at pH range between 4.2 and 9.4. It still remained about 80% of its activity at pH 9.4 under 50 ℃. It was stable at the pH ranging from 2.0 to 11.0, still possessing 75% of its maximal activity at pH 11 under 4 ℃ for 24 h. Fig 3, Tab 1, Ref 12

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更新日期/Last Update: 2008-07-01