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[1]雷美华,叶冰莹,王冰梅,等.甘蔗蔗糖合成酶基因的克隆[J].应用与环境生物学报,2008,14(02):177-179.
 LEI Meihua,et al..Cloning of Sucrose Synthase Gene from Sugarcane (Saccharum officenarum L.)[J].Chinese Journal of Applied & Environmental Biology,2008,14(02):177-179.
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甘蔗蔗糖合成酶基因的克隆()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
14卷
期数:
2008年02期
页码:
177-179
栏目:
综述
出版日期:
2008-02-15

文章信息/Info

Title:
Cloning of Sucrose Synthase Gene from Sugarcane (Saccharum officenarum L.)
作者:
雷美华叶冰莹王冰梅黄祖新张华许莉萍陈由强陈如凯
Author(s):
LEI Meihuaet al.
关键词:
关键词 甘蔗蔗糖合成酶基因(SuSy2)克隆氨基酸
Keywords:
Keywords sugarcane sucrose synthase (SuSy2) gene cloning amino acid
Abstract:
Abstract Sucrose synthase 2 (SuSy2) is a key enzyme regulating sucrose metabolism in higher plants, which promotes sucrose into various metabolic pathways. The degenerated oligonucleotides to highly conserved region of SuSy2 were used to prime the amplification of specific fragment by PCR in samples of sugarcane DNA. One fragment of about 7.5 kb was cloned and its complete nucleotide sequence was determined. Its nucleotide sequence and the protein from its coding region was 99.5% and 100% identical to the deduced proteins from the sugarcane SuSy2 cDNA. The sequence also included about 1.9 kb upstream promoter regulation,sixteen extrons, fifteen introns, 3- untranslatded region, and the open read frame coded 802 amino acids. The aminoacid sequence had a conservative sucrose metabolism key enzyme family Ser/Thr bit point.All that laid the foundation for its transcriptional expression regulation mechanism. Fig 2, Ref 19

相似文献/References:

[1]罗俊,王清丽,张华,等.不同甘蔗基因型光合特性的数值分类[J].应用与环境生物学报,2007,13(04):461.
 LUO Jun,et al..Phenetic Classification for Photosynthetic Characters of Different Sugarcane Varieties[J].Chinese Journal of Applied & Environmental Biology,2007,13(02):461.

更新日期/Last Update: 2008-04-23