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[1]张常洪 张英 朱晓敏** 尹华军.西南山地针叶林根际/菌丝际土壤氮动态对氮添加的响应差异[J].应用与环境生物学报,2022,28(06):1-10.[doi:10.19675/j.cnki.1006-687x.2021.11022]
 ZHANG Changhong,ZHANG Ying,et al.Differential responses of nitrogen dynamics between the r hizosphere and hyphosphere soils of mountain coniferous forests to N addition in southwest China[J].Chinese Journal of Applied & Environmental Biology,2022,28(06):1-10.[doi:10.19675/j.cnki.1006-687x.2021.11022]
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西南山地针叶林根际/菌丝际土壤氮动态对氮添加的响应差异()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
28卷
期数:
2022年06期
页码:
1-10
栏目:
研究论文
出版日期:
2022-12-25

文章信息/Info

Title:
Differential responses of nitrogen dynamics between the r hizosphere and hyphosphere soils of mountain coniferous forests to N addition in southwest China
作者:
张常洪12 张英12 朱晓敏** 尹华军1
1中国科学院成都生物研究所,中国科学院山地生态恢复与生物资源利用重点实验室,生态恢复与生物多样性保育四川省重点实验室 成都 610041
2中国科学院大学 北京 100049
Author(s):
ZHANG Changhong1 2 ZHANG Ying1 2 ZHU Xiaomin1** & YIN Huajun 1
1 CAS Key Laboratory Ecological Restoration and Bioresource Utilization & Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China
2 University of Chinese Academy of Sciences, Beijing 100049, China
关键词:
外生菌根根际菌丝际N转化土壤酶活土壤微生物群落N沉降
Keywords:
ectomycorrhiza rhizosphere hyphosphere N transformation soil enzyme activities soil microbial community composition N deposition
DOI:
10.19675/j.cnki.1006-687x.2021.11022
摘要:
尽管森林菌根共生体对土壤氮(N)循环过程具有重要的调控作用,但N沉降下森林根系和外延菌丝是否以及如何差异化地调控土壤N素动态仍知之甚少。以西南山地两种典型的人工针叶林——华山松(Pinus armandii)和云杉林(Picea asperata)为对象,依托研究团队建立的N添加试验样地,采用不同孔径的内生长管从物理上区分根(菌根)际和菌丝际,重点比较了根际/菌丝际土壤N动态对N添加的响应方向(促进或抑制)与幅度及其潜在微生物调控因素差异。结果发现:N添加显著抑制了华山松林和云杉林根际有机N矿化酶活性(N-乙酰葡萄糖苷酶,蛋白酶,脲酶活性分别降低38%和68.3%,54.5%和58.9%,43.2%和50.9%),导致两种人工林根际土壤净氨化速率分别降低了114.8%和101.7%。此外,N添加下,伴随着土壤革兰氏阴性细菌的增加(增幅为93.1%和81.9%),两种人工林根际土壤硝化速率分别增加了127.3%和95.7%。相应地,两种人工林根际土壤NO3-分别增加了117%和101.6%。相反,两种人工林菌丝际土壤N库,净N转化速率及其相关微生物特征在N添加后均无显著变化。上述结果表明西南山地针叶林根际土壤N过程对短期N沉降的响应比菌丝际土壤更加敏感。(图7参57)
Abstract:
Although mycorrhizal symbiont plays a vital role in regulating soil nitrogen (N) cycling, whether and how roots and ectomycorrhizal? mycelia differentially regulate soil N dynamics under N deposition remains poorly understood. In this study, a simulated N deposition experiment was conducted in two typical coniferous plantations (i.e., the Pinus armandii and the Picea asperata plantations ) in the southwest mountainous, China. In-growth cores with different mesh size were used to physically distinguish the rhizosphere and the hyphosphere. We focused on comparing the direction and magnitude of soil N dynamics in response to N addition between the rhizosphere and hyphosphere and the potential microbial regulatory factors. The results showed that N addition significantly inhibited the activities of N-mineralization enzymes in the rhizosphere (The activities of β-1, 4-N-acetylglucosamine glycosidase, protease, urease were significantly decreased by 38.0% and 68.3%, 54.5% and 58.9%, 43.2% and 50.9%, respectively, in the Pinus armandii and the Picea asperata plantations ), leading to a significant decrease of net ammonification rates by -114.8% and -101.7% compared to the control, respectively. In addition, the nitrification rates in the rhizosphere of the two plantations were increased by 127.3% and 95.7% compared to the controls, respectively, accompanying with the increased gram-negative bacterial biomass in the rhizosphere under N addition. As a result, the NO3- concentration in the rhizosphere of two plantations was increased by 117% and 101.6% compared to the control, respectively. On the contrary, N addition had no significant effect on soil N pools, net N transformation rates and associated microbial characteristics in the hyphosphere soil. These results suggest that N dynamic in the rhizosphere soil is more sensitive to short-term N deposition than that in the hyphosphere soil, highlighting the importance of understanding mycorrhizal interactions effect on soil nutrient cycling under future nitrogen deposition.

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更新日期/Last Update: 2022-03-16