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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:

28卷

期数:

2022年06期

页码:

1-10

栏目:

研究论文

出版日期:

2022-12-25

文章信息/Info

Title:

The degradation of phenol and cyanide by Alcaligenes faecalis JQBF100

作者:

蒋郑峰 陈昱光 蒋汉语 刘幽燕 李青云**

广西大学化学化工学院 南宁 530004

Author(s):

JIANG Zhengfeng;  CHENG Yuguang;  JIANG Hanyu;  LIU You- yang;  LI Qingyun**

School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China

关键词:

粪产碱杆菌; 苯酚; 氰化物; 动力学; 抑制作用

Keywords:

Alcaligenes faecalis;  phenol;  cyanide;  kinetics;  inhibitory effect

DOI:

10.19675/j.cnki.1006-687x.2021.11005

摘要:

焦化废水中的苯酚和氰化物都是难以生物降解的有毒环境污染物. 对前期筛选获得的1株降解菌Alcaligenes faecalis JQBF100开展苯酚、氰化物降解的动力学研究. 结果表明，采用Haldane底物抑制动力学模型可以较好地描述菌株对苯酚、氰化物的降解，在苯酚浓度为10-1000 mg/L、氰化物浓度为5-300 mg/L的单底物体系，菌株的最大比生长速率μmax分别为0.173 h-1、0.316 h-1，抑制常数KS分别为134.04 mg/L 、197.31 mg/L，苯酚对菌株的降解表现出较显著的抑制作用. 采用SKIP模型对苯酚、氰化物双底物的降解进行预测，拟合得到交互系数I酚,氰与I氰,酚的值分别为2.82和0.80，反映双底物体系中苯酚对菌株降解氰化物的抑制作用相对更大. 此外，对苯酚、氰化物双底物降解过程进行分析发现，菌株在双底物胁迫条件下会产生胞外聚合物EPS，并且氰化物的降解产物甲酰胺和甲酸可以被细胞利用生长，在苯酚-甲酰胺、苯酚-甲酸体系，菌株对800 mg/L苯酚的降解效率比单独降解时分别提高约41%和14%. 本研究表明在苯酚-氰化物双底物体系中菌株Alcaligenes faecalis JQBF100能快速降解氰化物，并且氰的解毒有利于加快菌株对苯酚的降解. （表1 图2 参15）

Abstract:

Phenol and cyanide in coking wastewater are both toxic environmental pollutants and are difficult to be biodegraded. In this paper, t he degradation kinetics of phenol and cyanide by Alcaligenes faecalis JQBF100 , previously isolated, were studied . The results showed that the Haldane inhibition kinetic model could be used to describ e the degradation inhibitory effects of phenol and cyanide by JQBF100. In the single-substrate system of 10 -1000 mg/L phenol and 5-300 mg/L cyanide, the maximum specific growth rate (μmax) were 0.173 h-1 and 0.316 h -1 respectively , and the inhibition constants were 134.04 mg/L and 197.31 mg/L, implying that the inhibitory effect of phenol on the strain was more obvious. In the dual-substrate system, the SKIP model was used to uate the interaction effects between phenol and cyanide. The fitted interaction coefficients I phenol,cyanide and I cyanide,phenol were 2.82 and 0. 80, respectively, suggesting that relatively greater inhibitory effect of phenol on cyanide degradation. Additionally, Alcaligenes faecalis JQBF100 was found to produce extracellular polymeric substance (EPS) in the dual-substrate stresses, and it could use the cyanide degradation products of formamide and formic acid to grow . In comparison with phenol degradation alone, t he degradation efficiencies of 800 mg/L phenol were increased by around 41% and 14% for the system of phenol- formamide and phenol-formic acid , respectively. The present study showed that cyanide could be rapidly degraded by Alcaligenes faecalis JQBF100 in the dual-substrate system of phenol and cyanide, and the detoxification of cyanide would be beneficial to accelerate phenol degradation.

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更新日期/Last Update: 2022-03-16