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[1]唐楚珺 高李文 彭紫薇 刘雨晖 陈辉 王玉哲 胡亚林 刘先**.连栽杉木人工林土壤氮循环功能基因丰度特征[J].应用与环境生物学报,2022,28(05):1-10.[doi:10.19675/j.cnki.1006-687x.2021.09003]
 TANG Chujun,GAO Liwen,PENG Ziwei,et al.Changes in relative abundance of functional genes related to nitrogen cycling in continuously cultivated Chinese fir (Cunninghamia lanceolate) plantations[J].Chinese Journal of Applied & Environmental Biology,2022,28(05):1-10.[doi:10.19675/j.cnki.1006-687x.2021.09003]
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连栽杉木人工林土壤氮循环功能基因丰度特征()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
28卷
期数:
2022年05期
页码:
1-10
栏目:
出版日期:
2022-10-25

文章信息/Info

Title:
Changes in relative abundance of functional genes related to nitrogen cycling in continuously cultivated Chinese fir (Cunninghamia lanceolate) plantations
作者:
唐楚珺1 高李文1 彭紫薇1 刘雨晖2 陈辉2 王玉哲1 胡亚林1 刘先1**
1福建农林大学林学院 福州 350002
2福建农林大学莘口教学林场 三明 365002
Author(s):
TANG Chujun1 GAO Liwen1 PENG Ziwei1 LIU Yuhui2 CHEN Hui2 WANG Yuzhe1 HU Yalin1 & LIU Xian1**
1 College of Forestry, Fujian Agriculture and Forestry University , Fuzhou 350002, China
2 Xinkou forest farm of Fujian Agriculture and Forestry University , Sanming 365002, China
关键词:
连栽杉木人工林氮循环功能基因丰度
Keywords:
continuous planting Chinese fir plantation nitrogen cycling functional genes abundance
DOI:
10.19675/j.cnki.1006-687x.2021.09003
摘要:
研究不同连栽代数杉木人工林土壤氮循环功能基因丰度的变化特征及其调控因子,以期为亚热带人工林定向调控土壤氮(N)素转化过程,提高N素利用效率并减少其负面效应提供科学依据。以中亚热带不同连栽代数(一代FRP、二代SRP、三代TRP)杉木人工林和格氏栲天然林(NF)为研究对象,采用荧光定量PCR技术测定冬季和夏季土壤细菌(16S rRNA )、真菌(18S rRNA)和参与氮循环功能基因AOA、AOB、nifH、nirK、nirS、nosZ丰度,并分析其与土壤理化因子的相关关系。结果显示,天然林转变为杉木人工林后,整体上各氮循环功能基因丰度在天然林与一代林之间无显著差异。不同连栽代数杉木林土壤AOA和AOB基因丰度在两季变化规律一致,AOA基因丰度随连栽代数增加呈显著递减趋势(P < 0.05 ),AOB基因丰度无显著差异。连栽杉木林土壤16S rRNA、18S rRNA、nifH、nirK、nirS和nosZ基因丰度变化特征因季节而异,冬季土壤nifH(7.05 - 8.15 copies/g)、nirS(6.78 - 8.08 copies/g)和nosZ(7.39 - 8.08 copies/g)基因丰度随连栽代数增加而下降;夏季土壤nirK(8.26 - 9.26 copies/g)基因丰度随连栽代数增加而先增加后下降;杉木林土壤细菌16S(10.36 - 11.03 copies/g)基因拷贝数高于真菌18S(7.34 - 8.20 copies/g)基因拷贝数,且二者均为夏季略高于冬季。土壤含水量、可溶性有机氮和硝态氮可以较大程度地解释土壤功能基因丰度的变化。本研究表明,亚热带杉木人工林中,土壤AOA相比于AOB在数量上更占据优势地位,且其丰度随着连栽代数增加呈下降趋势,说明硝化作用可能随连栽代数增加而减弱,这也许是土壤系统在氮素匮乏的条件下所采取的一种保氮策略。
Abstract:
This study investigated soil nitrogen (N) cycling functional gene abundance and its driving factors in different generations of Chinese fir plantations. We aimed to achieve the artificially regulation of N transformation process, enhance the N use efficiency and decrease the negative effect. The continuously cultivated Chinese fir plantations (first-generation (FRP), second-generation (SRP) and third-generation (TRP)) and Castanopsis kawakamii?natural forest (NF) in the mid-subtropics were selected for the study. Gene copy numbers of 16S rRNA, 18S rRNA, AOA, AOB, nifH, nirk, nirS and nosZ were measured using Q-PCR technique. The correlation between soil functional gene abundance and physiochemical parameters were also analyzed. The results showed that after conversion from natural forest to Chinese fir plantations, there was no significant difference between NF and FRP in the abundance of N–cycling functional genes. Soil AOA and AOB gene abundances exhibited similar trends across different generations in both seasons. With the increase of planting generations, the abundance of soil AOA in winter and summer both decreased sharply (P < 0.05 ), while the abundance of soil AOB remained largely unchanged. The variations in abundances of 16S rRNA, 18S rRNA, nifH, nirK, nirS and nosZ genes among different generations were season-specific. In winter, gene abundances of nifH (7.05 - 8.15 copies/g dry soil), nirS (6.78 - 8.08 copies/g dry soil) and nosZ (7.39 - 8.08 copies/g dry soil) decreased with increasing planting generation. In summer, nirK (8.26 - 9.26 copies/g dry soil) gene copy number firstly increased and then decreased with increasing planting generations. The 16S rRNA (10.36 - 11.03 copies/g dry soil) gene copy number was higher than that of 18S rRNA (7.34 - 8.20 copies/g dry soil) in all stands, and both were slightly higher in summer than in winter. Soil moisture content, soluble organic nitrogen and nitrate nitrogen are responsible for, to a large extent, the changes in soil functional genes. In subtropical Chinese fir plantations, soil AOA abundance was significantly higher than that of AOB, and soil AOA abundance significantly decreased with increasing plantation generations, indicating that nitrification weakened as the plantation generation increased. This might be a nitrogen-preserving strategy for adapting the nitrogen-deficient status.

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更新日期/Last Update: 2021-11-24