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[1]朱文秀 任洪艳** 庄海浪 朱国庆.污泥脱水液培养微藻在盐胁迫下的油脂积累情况[J].应用与环境生物学报,2021,27(04):1-13.[doi:10.19675/j.cnki.1006-687x.2020.09024]
 ZHU Wenxiu,REN Hongyan,et al.The lipid accumulation of cultivating microalgae usingsludge liquor?under salt stress[J].Chinese Journal of Applied & Environmental Biology,2021,27(04):1-13.[doi:10.19675/j.cnki.1006-687x.2020.09024]
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污泥脱水液培养微藻在盐胁迫下的油脂积累情况()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
27卷
期数:
2021年04期
页码:
1-13
栏目:
研究论文
出版日期:
2021-08-25

文章信息/Info

Title:
The lipid accumulation of cultivating microalgae usingsludge liquor?under salt stress
作者:
朱文秀12 任洪艳12** 庄海浪12 朱国庆12
1江南大学环境与土木工程学院 无锡 214122
2江苏省生物质能与减碳技术工程实验室 无锡 214122
Author(s):
ZHU Wenxiu1 2 REN Hongyan1 2** Zhuang Hailang 1 2 & Zhu Guoqing1 2
1 School of Environment Science and Civil Engineering, Jiangnan University, Wuxi 214122, China
2 Jiangsu Engineering Laboratory for Biomass Energy and Carbon Reduction Technology, Wuxi 214122, China
关键词:
微藻盐胁迫污泥脱水液粗甘油油脂积累
Keywords:
microalgae salt stress sludge liquor crude glycerol lipid accumulation
DOI:
10.19675/j.cnki.1006-687x.2020.09024
摘要:
为降低富油微藻制备生物柴油的成本,选取污泥脱水液作为培养液,并选取生物柴油副产物——粗甘油(实验室配制)作为外加碳源。考察不同粗甘油添加量、不同盐浓度和盐胁迫时间下的油脂积累情况。结果表明,在添加了粗甘油的污泥脱水液中,微藻长势良好,短期内即可进入稳定期,在1 g/L粗甘油添加浓度下生物量产率和油脂产量可达0.89 g L-1 d-1和0.62 g/L,分别为未添加组的1.95倍和1.88倍;对污泥脱水液中微藻施加盐胁迫,随着盐胁迫时间的增长,微藻的油脂含量随之上升。当NaCl添加量为20 g/L胁迫培养48 h时,可获得最大油脂含量、油脂产量和最高油脂得率,分别为45.4%、1.29 g/L和1.047 g/g,为对照组的1.97、1.65和2.64倍,对脱水液中COD和氨氮的利用率分别为85.3%和99.6%。经脂肪酸分析,20 g/L NaCl 组的不饱和脂肪酸含量为78.9%,高于未添加组,符合生物柴油生产要求,可作为生物柴油生产原料。研究表明,污泥脱水液中外加粗甘油作为碳源,可优化C/N,促进微藻生长;对脱水液中微藻施加盐胁迫,在适宜的盐浓度和胁迫时间下,可显著提高藻细胞的油脂含量,促进油脂积累,为规模化培养微藻产生物柴油提供一定参考。(图4表4参39)
Abstract:
In order to reduce the cost of producing biodiesel from oil-rich microalgae, the sludge liquor was selected as the culture solution, and the biodiesel by-product—crude glycerol ( prepared in laboratory) was selected as the external carbon source. The lipid accumulation under different crude glycerol dosage , different salt concentrations and salt stress time were investigated. The results showed that in the sludge liquor supplemented with crude glycerol, microalgae grew well and could enter a stable growth period in a short time. Under the concentration of 1 g/L crude glycerol, the biomass yield and lipid production could reach 0.8 9 g L-1 d-1 and 0.62 g/L, which were 1.95 and 1.88 times of those in the untreated group respectively. After adding NaCl to sludge liquor , with the increase of culture time, the lipid content of microalgae increased. When NaCl was added to 20 g/L and culturing microalgae for 48 h, the maximum lipid content, lipid production and lipid yield were obtained, respectively 45.4%, 1.29 g/L and 1.047 g/g, which were 1.97, 1.65 and 2.64 times as much as those in the control group. And the utilization rate of COD and ammonia nitrogen in sludge liquor was 85.3% and 99.6% . According to fatty acid analysis, the content of unsaturated fatty acid in the 20 g/L NaCl group was 78.9%, higher than that in the non-added group, which met the requirements for biodiesel production and could be used as raw material for biodiesel production. The results showed that adding crude glycerol as carbon source in sludge liquor could optimize C/N and promote the growth of microalgae; salt stress on microalgae in sludge liquor could significantly increase the lipid content of algae cells and promote the accumulation of lipid under the appropriate salt concentration and stress time, which provided a certain reference for large-scale cultivation of microalgae to produce biodiesel.

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备注/Memo

备注/Memo:
收稿日期 Received: 2020-09-14 接受日期 Accepted: 2020-10-26
国家自然科学青年基金项目(21808087)资助
**通讯作者 Corresponding author (E-mail: renhongyan@jiangnan.edu.cn)
本文为已录用的作者修定稿,尚未经编辑全面修改。
引用本文请注明出处本刊;发表刊期和页码将以正式出版时的安排为准,但DOI确定不变。

更新日期/Last Update: 2020-11-06