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[1]陈玉凤 李竹 苏炜华 孙婷婷 汪洲涛 岑光莉 阙友雄 蒙寅恬 许莉萍 苏亚春*.甘蔗AP2/ERF基因家族的鉴定及表达[J].应用与环境生物学报,2021,27(04):1-20.[doi:10.19675/j.cnki.1006-687x.2020.09016]
 CHEN Yufeng,LI Zhu,SU Weihua,et al.Identification and expression analysis of AP2/ERF gene family in sugarcane[J].Chinese Journal of Applied & Environmental Biology,2021,27(04):1-20.[doi:10.19675/j.cnki.1006-687x.2020.09016]
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甘蔗AP2/ERF基因家族的鉴定及表达()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
27卷
期数:
2021年04期
页码:
1-20
栏目:
研究论文
出版日期:
2021-08-25

文章信息/Info

Title:
Identification and expression analysis of AP2/ERF gene family in sugarcane
作者:
陈玉凤1 李竹1 苏炜华1 孙婷婷1 汪洲涛1 岑光莉1 阙友雄12 蒙寅恬1 许莉萍12 苏亚春12*
1福建农林大学农业农村部福建甘蔗生物学与遗传育种重点实验室 福州 350002
2福建农林大学教育部作物遗传育种与综合利用重点实验室 福州 350002
Author(s):
CHEN Yufeng1 LI Zhu1 SU Weihua1 SUN Tingting1 WANG Zhoutao1 CEN Guangli1 QUE Youxiong1 2 MENG Yintian1 XU Liping1 2 & SU Yachun 1 2*
1 Key Laboratory of Sugarcane Biology and Genetic Breeding (Fujian), Ministry of Agriculture and Rural Affairs, Fujian Agriculture and Forestry University, Fuzhou 350002, China
2 Key Laboratory of Crop Genetics and Breeding and Comprehensive Utilization, Ministry of Education, College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, China
关键词:
割手密AP2/ERF基因家族ERF亚家族全基因组分析表达模式
Keywords:
Saccharum spontaneum AP2/ERF gene family ERF subfamily genome-wide analysis expression pattern analysis
DOI:
10.19675/j.cnki.1006-687x.2020.09016
摘要:
AP2/ERF(APETALA2/ethylene-responsive element binding factor)是植物中最大的转录因子家族之一,在植物的生长发育、生物和非生物逆境响应中发挥重要作用。为了解甘蔗AP2/ERF基因家族的生物学特性,本研究利用生物信息学方法从甘蔗野生种割手密(Saccharum spontaneum)基因组挖掘获得121条SsAP2/ERF基因序列,对其编码蛋白的理化性质、系统进化树、染色体定位和基因结构等进行分析。此外,从我国大陆主栽甘蔗品种ROC22中克隆获得1条SsAP2/ERF-107的同源基因ScDREB2B-2,并分析了其表达模式。结果显示,SsAP2/ERF家族基因分布于31条割手密染色体上,且存在基因聚集现象,属于AP2、ERF和RAV亚家族的基因数目分别为28、86和7。SsAP2/ERF-001~SsAP2/ERF-121蛋白之间的分子量差异较大,介于16 548.18 Da~609 263.26 Da ,不含信号肽,且99.17%无跨膜螺旋结构,含有3~10个保守基序和1个以上的外显子,推测大多数蛋白为定位于细胞核或细胞质的不稳定的亲水性蛋白。SsERF亚家族包含42个SsDREBs和44个SsERFs亚组成员,其AP2结构域的第14位氨基酸残基具有极高的保守性。克隆获得DREB(A-2)亚组成员SsAP2/ERF-107的同源基因ScDREB2B-2,两者氨基酸序列相似性达97.58%。qRT-PCR分析显示,ScDREB2B-2基因在ROC22中受PEG、NaCl和ABA诱导上调表达;在Badila中仅受PEG和NaCl诱导上调表达,而在ABA处理下的基因表达量不变,表明该基因在ROC22中可能通过ABA依赖性途径调节应答干旱和盐胁迫,而在Badila中可能通过非ABA依赖性途径调节响应干旱和盐胁迫。研究结果可为探究甘蔗SsAP2/ERF基因家族的结构和功能提供科学依据。(图8 表1参68)
Abstract:
AP2/ERF (APETALA2/ethylene responsive element binding factor) is one of the largest transcription factor families in plants, which plays an important role in plant growth, development, and biotic and abiotic stresses response. In order to understand the biological characteristics of AP2/ERF gene family in sugarcane, 121 SsAP2/ERF genes were obtained from the genome of sugarcane wild species Saccharum spontaneum by bioinformatics method. The physicochemical property, phylogenetic tree, chromosomal localization, and gene structure of the se SsAP2/ERF s were analyzed. Besides, a homologous gene of SsAP2/ERF-107, ScDREB2B-2, was cloned from the prent sugarcane cultivar ROC22 in mainland China , and its expression pattern was detected. The results showed that, the SsAP2/ERF family genes were distributed on 31 chromosomes of S. spontaneum with a phenomenon of gene aggregation. The number of genes belonging to AP2, ERF, and RAV subfamilies were 28, 86, and 7, respectively. The molecular weight of SsAP2/ERF-001 –SsAP2/ERF-121 proteins varied greatly, ranging from 16 548.18 Da to 609263.26 Da. In SsAP2/ERF family proteins, there was no signal peptide, and 99.17% of them had no transmembrane helix structure. SsAP2/ERF family proteins contained 3–10 conserved motifs as well as more than one exon. It was speculated that most of the SsAP2/ERFs were unstable and hydrophilic proteins located in the nucleus or cytoplasm. The SsERF subfamily contained 42 SsDREBs and 44 SsERFs subgroup members, and the 14th amino acid residue of AP2 domain was highly conserved. ScDREB2B-2, a member of DREB (A-2) subgroup, was then cloned. The amino acid sequence similarity of ScDREB2B-2 and its homologous gene SsAP2/ERF-107 was 97.58%. qRT-PCR analysis demonstrated that the expression of ScDREB2B-2 gene could be up-regulated by PEG, NaCl, and ABA in ROC22 but only by PEG and NaCl in Badila, suggesting that this gene may regulate the response to drought and salt stresses through an ABA-dependent pathway in ROC22, however by an ABA-independent pathway in Badila. e Thes results provide a scientific basis for exploring the structure and function of SsAP2/ERF gene family in sugarcane.

备注/Memo

备注/Memo:
收稿日期 Received: 2020-09-08 接受日期 Accepted: 2020-10-26
国家重点研发计划项目(2018YFD1000503,2019YFD1000500)、国家自然科学基金项目(31671752)和国家糖料产业技术体系(CARS-17)资助
*通信作者Corresponding author (E-mail: syc2009mail@163.com)
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更新日期/Last Update: 2020-11-06