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[1]曹慧丽 樊丹丹 姚敏杰 李香真**.【综述】土壤反硝化过程速率测定方法[J].应用与环境生物学报,2021,27(04):1-13.[doi:10.19675/j.cnki.1006-687x.2020.09007]
 CAO Huili,FAN Dandan,YAO Minjie & LI Xiangzhen**.Methods for measuring soil denitrification rate[J].Chinese Journal of Applied & Environmental Biology,2021,27(04):1-13.[doi:10.19675/j.cnki.1006-687x.2020.09007]
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【综述】土壤反硝化过程速率测定方法()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
27卷
期数:
2021年04期
页码:
1-13
栏目:
综 述
出版日期:
2021-08-25

文章信息/Info

Title:
Methods for measuring soil denitrification rate
作者:
曹慧丽1 樊丹丹1 姚敏杰1 李香真2**
1福建农林大学资源与环境学院,土壤修复福建省高校工程中心 福州 350002
2中国科学院环境与应用微生物重点实验室,环境微生物四川省重点实验室,中国科学院成都生物研究所 成都 610041
Author(s):
CAO Huili1 FAN Dandan1 YAO Minjie1 & LI Xiangzhen2**
1 Engineering Research Center of Soil Remediation of Fujian Province University, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002,China
2 Key Laboratory of Environmental and Applied Microbiology, CAS, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China
关键词:
土壤反硝化过程反硝化测定方法N2O乙炔抑制法15N同位素示踪法N2直接测定法
Keywords:
soil denitrification N2O acetylene inhibition method 15N isotope tracer method direct N2 quantification
DOI:
10.19675/j.cnki.1006-687x.2020.09007
摘要:
土壤反硝化过程是指土壤中的硝酸盐、亚硝酸盐等含氮物质在反硝化微生物的作用下还原成氮气(N2)、一氧化氮(NO)、氧化亚氮(N2O)等气体的过程,是氮循环中重要的过程之一。反硝化的中间产物N2O是一种重要的温室气体,其中从土壤中释放的量占地球总排放量的70%。反硝化作用主要由硝酸盐还原酶(Nitrate reductase, Nar)、亚硝酸还原酶((Nitrite reductase, Nir)、一氧化氮还原酶(Nitric oxide reductase, Nor)和氧化亚氮还原酶(Nitrous oxide reductase, Nos)所催化,相应的编码基因分别为nar、 nir、nor和nos。土壤反硝化速率测定方法有很多种,不同的方法因技术、设备、实验设计等原因存在不同的问题,因此了解不同方法的优缺点,在自己的研究中选择合适的方法至关重要。目前,测定土壤反硝化的方法主要有(1)乙炔抑制法;(2)15N同位素示踪法;(3)N2直接测定法;(4)硝酸盐消失法;(5)质量守恒法;(6)化学计量法等。每种方法都有其优势和局限性:乙炔抑制法,操作简单,但是不适合土壤养分含量低的土壤;15N同位素示踪法测定结果比较精确,但是价格昂贵,成本高;N2直接测定法则需要精密的仪器。今后,反硝化过程测定方法的发展不仅是测量方法的改进,也需要注重精密仪器的研发。(表3参62)
Abstract:
Soil denitrification refers to the process of reducing nitrate, nitrite and other nitrogen-containing substances in the soil into nitrogen (N2), nitric oxide (NO) and nitrous oxide (N2O) gas catalyzed by denitrifying microorganism s. Denitrification is one of most important processes in nitrogen cycle. N 2O, an intermediate product of denitrification, is an important greenhouse gas, and its emission from soil accounts for 70% of total global emissions. Denitrification is mainly catalyzed by Nitrate reductase (Nar), Nitrite reductase ( Nir), Nitric oxide reductase (Nor) and Nitrous oxide reductase (Nos) , and the corresponding coding genes are nar, nir, nor and nos. There are some methods for determining soil denitrification rate, and each has its own specific problems due to used technique, equipment and experimental design. Therefore, it is important to understand the advantages and disadvantages of various methods and choose appropriate method in our research work . Currently, the main methods for measuring soil denitrification include (1) acetylene inhibition method, (2) 15N isotope tracer method, (3) direct N2 quan tification, (4) nitrate disappearance method, (5) mass balance approaches, (6) stoichiometry approaches etc.. Each method has its own advantages and limitations. Acetylene inhibition method is cheap and easy to operate, but it is not suitable for nutrient -poor soil; 15N isotope tracer method is accurate, but it is expensive; Direct N2 quantification requires sophisticated equipments. Future development of denitrification method include the improvement of measurement procedures and the development of precision instruments.

备注/Memo

备注/Memo:
收稿日期 Received: 2020-09-06 接受日期 Accepted: 2020-11-06
国家自然科学基金面上项目(32071548, 44077206, 31670503)、国家重点研发计划(2018YFE0107000)、生物多样性监测网络(Sino BON)资助。The National Natural Science Foundation of China (32071548,44077206,31670503);Research and Development Program of China (grant no. 2018YFE0107000 ), and China Biodiversity Observation Networks (Sino BON).
**通讯作者Corresponding author(E-mail: lixz@cib.ac.cn)
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更新日期/Last Update: 2020-11-06