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[1]乔自鹏 张君毅 王明元 刘建福 王奇志**.一株胜红蓟内生真菌Letendraea sp. WZ07的鉴定、培养条件与抗氧化活性[J].应用与环境生物学报,2021,27(04):1-13.[doi:10.19675/j.cnki.1006-687x.2020.09001]
 QIAO Zipeng,ZHANG Junyi,WANG Mingyuan,et al.Identification of endophytic fungi Letendraea sp. WZ07 from Ageratum conyzoides and its fermentation extract a ntioxidant activity[J].Chinese Journal of Applied & Environmental Biology,2021,27(04):1-13.[doi:10.19675/j.cnki.1006-687x.2020.09001]
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一株胜红蓟内生真菌Letendraea sp. WZ07的鉴定、培养条件与抗氧化活性()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
27卷
期数:
2021年04期
页码:
1-13
栏目:
研究论文
出版日期:
2021-08-25

文章信息/Info

Title:
Identification of endophytic fungi Letendraea sp. WZ07 from Ageratum conyzoides and its fermentation extract a ntioxidant activity
作者:
乔自鹏 张君毅 王明元 刘建福 王奇志**
华侨大学化工学院 厦门 361021
Author(s):
QIAO Zipeng ZHANG Junyi WANG Mingyuan LIU Jianfu & WANG Qizhi**
College of Chemical Engineering, Huaqiao University, Xiamen 361021, China
关键词:
胜红蓟内生真菌分离鉴定培养条件黄酮和多酚抗氧化活性
Keywords:
Ageratum conyzoides L. endophytic fungi identification culture condition flavonoids phenolics antioxidant activity
DOI:
10.19675/j.cnki.1006-687x.2020.09001
摘要:
以药用植物胜红蓟茎部组织中分离获得的一株内生真菌并进行了形态学与ITS分类学鉴定;采用单因素实验及正交设计优化该菌株生长的最适条件;探究发酵液不同极性提取物(乙酸乙酯提取物EAE、乙醇提取物EE和石油醚提取物PEE)黄酮、多酚含量与其体外抗氧化活性(1,1-二苯基-2-三硝基苯肼(DPPH)、2,2’-二氮-双(3-乙基苯并噻唑-6-磺酸) (ABTS)自由基清除能力和Fe3+还原能力)的相关性。结果显示,该菌株为Letendraea sp. WZ07 ;WZ07菌丝生长的最适条件为:温度为28 ℃,pH为5.0,淀粉20 g/L,尿素0.5 g/L,磷酸二氢钾1 g/L,硫酸镁0.5 g/L,VB6或肌醇10 mg/L;3种有机溶剂提取物黄酮和多酚含量差异显著(P < 0.05),其中EAE含有的黄酮和多酚含量最高,分别为11.97 mg RE/g、14.70 mg GAE/g;提取物均具有清除DPPH、ABTS自由基和还原Fe3+的能力,且均呈量效关系,EAE抗氧化活性能力最强,在浓度为2 mg/mL 时,对清除DPPH自由基率可达87.23%,其IC50为242.13 μg/mL;对清除 ABTS 自由基率为94.72%,与阳性组维生素C清除能力相当,其IC50为58.38 μg/mL;对Fe3+还原力OD值为0.78,其IC50为325.17 μg/mL。相关性分析显示,不同提取物的黄酮和多酚含量均与抗氧化活性呈正相关关系,表明黄酮和多酚类成分可能是WZ07菌株起抗氧化活性的功效因子。本研究结果可为深入开发利用该真菌资源提供科学依据。(图7 表10 参51)
Abstract:
In this study, a pure culture strain (numbered as WZ07) from the stems of the medicinal plant Ageratum conyzoides L. was obtained using tissue separation and purification methods. The taxonomic characteristic was determined based on morphological and ITS sequence analysis. Optimal culture conditions of mycelia were uated based on their growth in different media. The correlation of the vitro antioxidant activity (DPPH radical scavenging, ABTS radical scavenging, Fe3+ reducing power assays) with the content of flavonoids compounds and polyphenols compounds in different polar extracts of fermentation broth (ethyl acetate extract EAE, ethanol extract EE and petroleum ether extract PEE) were studied. The results showed that the strain was classified as Letendraea sp. WZ07. The optimal conditions for mycelial growth were: temperature of 28 ℃, pH of 5.0, C/N ratio of 30/1, starch of 20 g/L , urea of 0.5 g/L , potassium dihydrogen phosphate of 1 g/L , magnesium sulfate of 0.5 g/L , VB6 and inositol of 10 m g/L. The content of flavonoids and phenolics in the three organic solvent extracts was different ( P <0.05) , of which EAE had the highest content of flavonoids(11.97 mg RE/g) and polyphenols (14.70 mg GAE/g). The antioxidant assays showed that the extracts of different organic solvents with the ability to scavenge DPPH and ABTS free radicals and reduce Fe3+, and showed a dose effect relationship, of which EAE had the strongest antioxidative activity. At the concentration of 2 mg/mL, the scavenging ability to DPPH was 87.23%(IC50 = 242.13 μg/mL), and the scavenging ability to ABTS radicals was 94.72% ( IC50= 58.38 μg/mL), which was equivalent to the ascorbic acid (VC) scavenging ability in positive group, the OD value for Fe3+ reducing power was 0.78(IC50 = 325.17 μg/mL). The correlation analysis showed that the content of flavonoids and phenolics is positively correlated with antioxidant activity in different extracts, which showed that flavonoids and polyphenols were dominant antioxidant factors. The results of this study provided a scientifc basis for the development and utilization of this fung i resources.

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备注/Memo

备注/Memo:
收稿日期 Received: 2020-09-02 接受日期 Accepted: 2020-10-15
2018年中医药公共卫生服务补助专项“全国中药资源普查项目”(财社〔2018〕43号)、福建省农业重点自然科学基金项目( 2014N0026)、泉州市校(院)地协同创新专项(重点)项目(2014Z101)、华侨大学研究生科研创新基金资助项目(18014087015)资助
**通信作者 Corresponding author(E-mail: wqz@hqu.edu.cn)
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更新日期/Last Update: 2020-11-06