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[1]李冠军 梁安洁 姚成硕 林勇明 李键**.盐胁迫对木麻黄内生真菌固体培养次生代谢产物的影响[J].应用与环境生物学报,2021,27(04):1-12.[doi:10.19675/j.cnki.1006-687x.2020.08052]
　LI Guanjun,LIANG Anjie,YAO Chengshuo,et al.Effect of salt stress on the secondary metabolites of endophytic fungi of Casuarina equisetifolia in solid culture[J].Chinese Journal of Applied & Environmental Biology,2021,27(04):1-12.[doi:10.19675/j.cnki.1006-687x.2020.08052]
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盐胁迫对木麻黄内生真菌固体培养次生代谢产物的影响()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
27卷
期数:
2021年04期
页码:
1-12
栏目:
研究论文
出版日期:
2021-08-25

文章信息/Info

Title:
Effect of salt stress on the secondary metabolites of endophytic fungi of Casuarina equisetifolia in solid culture
作者:
李冠军1; 2 梁安洁1; 2 姚成硕1; 2 林勇明1; 2 李键1; 2**
1福建农林大学林学院 福州 350002
2福建省高校森林生态系统过程与经营重点实验室 福州 350002
Author(s):
LI Guanjun1; 2;  LIANG Anjie1; 2;  YAO Chengshuo1; 2;  LIN Yongming1; 2 &LI Jian1; 2**
1 College of Forestry, Fujian Agriculture and Forestry University , Fuzhou 350002, China 2 Key Laboratory of Forest Ecosystem Process and Management of Fujian Province , Fuzhou 350002, China
关键词:
固体; 内生真菌; 载体优化; 次生代谢产物
Keywords:
solid;  endophytic fungi;  carrier optimization;  metabolite
DOI:
10.19675/j.cnki.1006-687x.2020.08052
摘要:
为探索合适的木麻黄内生真菌固化载体配比，以具有耐盐促生功能的木麻黄内生真菌（Y6 炭团菌属Hypoxylon、Z1 葡萄座腔菌属Botryosphaeria）为研究对象，通过单因素实验和正交实验优化固体发酵培养基组成，通过高效液相色谱仪（HPLC）和气相色谱-质谱联用仪（GC-MS）测定不同盐度固体培养基培养下内生真菌（Y6、Z1）及其混菌（1:1）次生代谢产物。结果显示：（1）优化的Y6菌培养基载体组合（质量比）为麦麸65%、豆饼粉15%、米糠10%、玉米芯粉10%，Z1菌培养基载体组合为麦麸65%、豆饼10%、米糠15%、玉米芯10%；（2）在高盐（6‰、9‰）培养条件下培养2株内生真菌及其混菌产生的3种植物激素含量总体高于低盐（3‰）培养条件，混菌在高盐度培养下产生的玉米素（t-z）、赤霉素（GA3）、吲哚乙酸（IAA）高于2种单菌株培养；（3）Y6菌、Z1菌及其混菌共有且相对含量较高的物质为cis-9-octadecenoic acid，Y6菌和混菌共有的代谢物质为L-(-)-arabitol，以及Z1菌生成的Z-9-hexadecenal、ethyl cis-9-octadecenoate、9-hexadecenoic acid, ethyl ester和混菌生成的Z-9-hexadecenal、n-tetratetracontane相对含量同样较高。本研究明确了适合木麻黄菌株生长的主要载体及配比，盐度胁迫、混合培养均能够改变内生真菌次生代谢产物的种类和含量；结果可为内生真菌固体菌肥的开发和理解其促生机制提供研究基础和经验累积，亦为海岸带森林可持续经营提供新的思路。（图5 表5 参50）
Abstract:
Research on the appropriate ratio of solidified carriers of endophytic fungi of Casuarina equisetifolia, the endophytic fungi (Y6 Hypoxylon, Z1 Botryosphaeria) with salt-tolerant and growth-promoting functions were research object. The composition of the solid fermentation medium was optimized by single factor experiment and orthogonal experiment, and metabolites of endophytic fungi (Y6, Z1) and mixed fungi (1:1) were determined by high performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS). The results showed that optimized fungi Y6 medium carrier combination (mass ratio) was wheat bran 65%, soybean meal 15%, rice bran 10%, corn cob flour 10%, fungi Z1 medium carrier combination was wheat bran 65%, bean cake 10%, rice bran 15%, corn cob flour 10%. The content of the three plant hormones produced by the two endophytic fungi and their mixed fungi under high salt (6 ‰, 9‰) culture was generally higher than that of low salt (3‰) culture, and the content of the three plant hormones produced by mixed fungi were higher than the two single fungi under high salt culture. The cis-9-octadecenoic acid was produced by fungi Y6, Z1 and mixed fungi, the L-(-)-arabitol was produced by fungi Y6 and mixed fungi, the relative content of Z-9-hexadecenal, Ethyl cis-9-octadecenoate, 9-Hexadecenoic acid, ethyl ester produced by Z1 fungi, and the Z-9-hexadecenal, n-tetratetracontane produced by mixed fungi were also higher. The main carriers and proportions suitable for the growth of fungi were clarified, salinity stress and mixed culture can change the types and contents of secondary metabolites of endophytic fungi, which can provide research foundation and experience accumulation for the development of solid fertilizers for endophytic fungi and understanding of the growth-promoting mechanism, and provide new ideas for sustainable management of coastal forests.

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备注/Memo

备注/Memo:
收稿日期 Received: 2020-08-31 接受日期 Accepted: 2020-09-30
国家自然科学基金项目（32071753）、福建农林大学科技创新专项基金项目（CXZX2016055，CXZX2018122）和福建农林大学林学院林学高峰学科建设项目（71201800705）资助
**通讯作者Corresponding author (E-mail: jianli@fafu.edu.cn)
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更新日期/Last Update: 2020-10-05