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[1]叶雷 谭伟 张伟伟 戴怀斌 张波 李小林**.毛木耳代料栽培适宜碳氮比及菌棒中固碳、固氮细菌群落结构[J].应用与环境生物学报,2021,27(04):1-13.[doi:10.19675/j.cnki.1006-687x.2020.08050]
 YE Lei,TAN Wei,ZHANG Weiwei,et al.The suitable carbon-nitrogen ratio on Auricularia cornea substitute cultivation and the community structure of carbon and nitrogen assimilating bacteria[J].Chinese Journal of Applied & Environmental Biology,2021,27(04):1-13.[doi:10.19675/j.cnki.1006-687x.2020.08050]
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毛木耳代料栽培适宜碳氮比及菌棒中固碳、固氮细菌群落结构()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
27卷
期数:
2021年04期
页码:
1-13
栏目:
研究论文
出版日期:
2021-08-25

文章信息/Info

Title:
The suitable carbon-nitrogen ratio on Auricularia cornea substitute cultivation and the community structure of carbon and nitrogen assimilating bacteria
作者:
叶雷12 谭伟12 张伟伟3 戴怀斌3 张波12 李小林12**
1 四川省农业科学院土壤肥料研究所 成都 610066
2 农业部西南区域农业微生物资源利用科学观测实验站 成都 610066
3 什邡市农业农村局 什邡 618400
Author(s):
YE Lei12 TAN Wei12 ZHANG Weiwei3 DAI Huaibin3 ZHANG Bo12 & LI Xiaolin 12**
1 Soil and Fertilizer Institute, Sichuan Academy of Agricultural Sciences , Chengdu 610066, China
2 Scientific Observing and Experimental Station of Southwestern Region Agromicrobiological Resource Utilization, Ministry of Agriculture , Chengdu 610066, China
3 Shifang Agricultural and Rural Bureau , Shifang 618400, China
关键词:
碳氮比毛木耳代料栽培农艺性状cbbL基因norB基因
Keywords:
carbon-nitrogen ratio Auricularia cornea Substitute cultivation Agronomic characters cbbL gene norB gene
DOI:
10.19675/j.cnki.1006-687x.2020.08050
摘要:
为解析毛木耳代料栽培基质碳氮比组成对其生育的影响以及在最适碳氮比的菌棒中参与卡尔文循环的固碳基因和固氮基因多样性,以毛木耳“上海1号”为供试菌株,设计5个不同碳氮比基质,在同一条件下进行出耳试验,对相关农艺性状指标进行测定,并采用高通量测序技术对最佳碳氮比处理组菌棒中固碳和固氮基因多样性进行分析。结果显示:(1)在碳氮比约60:1(C3)时最佳。菌丝长速最快为4.43 mm/d;料袋发菌污染率最低为4.27%;菌丝长势好;第一潮鲜耳单簇表面积达3 624.09 cm2/簇,干耳边缘厚达0.71 mm;单袋干耳单产达164.79 g/袋,转化率达14.98%,其次是45:1(C4)和75:1(C2)。(2)菌丝满袋期C3菌棒含cbbL固碳基因主要分布在Proteobacteria和Actinobacteria,相对丰度分别为90.8%和0.2%,且以β-Proteobacteria为主,主要归属于Cupriavidus 41.7% 、Rubrivivax 33.6%、Methylococcus 6.5% ;而含cbbM基因的固碳细菌未检测出。(3)norB固氮基因主要归属于Firmicutes 3.2% 和Proteobacteria 0.4% ,主要优势菌属为Geobacillus 2.1% ,Macrococcus 0.9% ,Pseudomonas 0.2% ,Staphylococcus 0.1% 和Bacillus 0.1% 等。本研究筛选获得了毛木耳代料栽培较佳基质碳氮比60:1,首次对菌丝满袋期参与固碳和固氮基因细菌群落进行了解析,为实现毛木耳精准化栽培和研究参与基质碳、氮素物质转化功能微生物菌群奠定了基础。(图5 表5 参38)
Abstract:
To interpretation the effect of matrix carbon-nitrogen ratio (C/N) on the growth and development of Auricularia cornea and the diversity of carbon-nitrogen assimilation genes involved in Calvin cycle (a common carbon fixation pathway for chemolithoautotrophic microorganisms). The "Shanghai No. 1" was used as the test strain. designed 5 substrate with different C/N and test them under the same conditions, agronomic characters were measured, the high-throughput sequencing technology was used to analyze the diversity of carbon- nitrogen fixation genes with the optimum C/N substrate. Results: (1) The suitable C/N was about 60:1 (C3), followed by 45:1 (C4) and 75:1 (C2). C3 was the best treatment, with the fastest mycelial growth rate 4.43 mm/d, the lowest contamination rate of bags 4.27%; the surface areas reach to 3,624.09 cm2/tuft (the fresh fruiting body of first harvested), edge thickness 0.71 mm (the dry fruiting body of first harvested); yield 164.79 g/bag (weight of dry fruiting bodies was harvested three times from one bag), converted efficiency 14.98%. (2) The cbbM gene was not detected in C3 samples, while the cbbL gens was found in C3 sample. The cbbL-containing genetic communities were dominated by Proteobacteria and Actinobacteria with relative abundances of 90.8% and 0.2%, respectively, in which β-Proteobacteria were dominant, mainly attributable to Cupriavidus 41.7%, Rubivivax 33.6% and Methylococcus 6.5%. (3) norB-containing genetic communities were dominated by Firmicutes 3.2% and Proteobacteria 0.4% , mainly including Geobacillus 2.1%, Macrococcus 0.9%, Pseudomonas 0.2%, Staphylococcus 0.1% and Bacillus 0.1%. In this paper, we obtained the best C/N (that is 60:1) for the substitute cultivation of Auricularia cornea . The bacterial community of participates in carbon-nitrogen assimilating were analyzed for the first time when the bag full of hyphae, and laying a foundation for the realization of accurate cultivation and the research on the functional microbial flora involved in the conversion of carbon-nitrogen in the Auricularia cornea substrate.

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备注/Memo

备注/Memo:
收稿日期 Received: 2020-08-31 接受日期 Accepted: 2020-10-10
国家食用菌产业技术体系毛木耳和药用菌栽培岗位(CARS-20)和四川食用菌创新团队资助项目(SCCXTD-2020-07)资助
**通讯作者 Corresponding author (E-mail: kerrylee_tw@sina.com)
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更新日期/Last Update: 2020-11-06