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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:

27卷

期数:

2021年04期

页码:

1-10

栏目:

研究论文

出版日期:

2021-08-25

文章信息/Info

Title:

Analysis of microbial community functional diversity in rare earth element, lead and fluorine compound polluted soil based on Biolog-ECO

作者:

王雪梅1; 2 黄利群1 刘成1 李佳芮1 胡金朝1 刘国2**

1西昌学院资源与环境学院 西昌 615013
2成都理工大学国家环境保护水土污染协同控制与联合修复重点实验室 成都 610059

Author(s):

WANG Xue-mei1; 2;  HUANG Li-qun1;  LIU Cheng1;  LI Jia-rui1;  HU Jin-zhao1;  LIU Guo2**

1 College of Resources and Environment, Xichang University , Xichang 615013, China
2 State Environmental Protection Key Laboratory of Synergetic Control and Joint Remediation for Soil &Water Pollution, Chengdu University of Technology, Chengdu 610059, China

关键词:

Biolog-ECO; 稀土、铅和氟复合污染; 土壤微生物; 群落功能多样性

Keywords:

Biolog-ECO;  rare earth complex pollution;  soil microorganism;  community functional diversity

DOI:

10.19675/j.cnki.1006-687x.2020.08015

摘要:

为考察四川省牦牛坪稀土矿区稀土、铅和氟复合污染对农田微生物群落功能多样性的影响，采用Biolog-ECO法分析稀土矿区高污染（H区）和低污染区（L区）农田土壤根际与非根际微生物群落功能多样性特征及其与土壤理化性质的相关关系。结果显示，不同污染区土壤微生物对 31 种碳源的利用能力存在显著性差异（P < 0.05），H区的非根际土壤微生物利用能力、多样性指数均最低，其AWCDi ≥ 0.8的碳源仅1种，而根际土壤有27种，占总碳源的87.1%，植物生长显著促进根际细菌活性；而L区土壤根际与非根际微生物差异显著的碳源仅5种（P < 0.05）。冗余分析（Redundancy Analysis，RDA）表明在非根际条件下稀土元素（REE）、铅、氟、碳氮比（C/N）与AWCD、Shannon指数等多样性指数呈负相关，其中REE贡献了87.4%的微生物群落特征解释率，是微生物群落变化的主要驱动因子。根际条件下4个环境因子仅解释了48%的特征值，说明植物的参与改变了污染物对微生物群落的影响。因此在高浓度稀土、铅和氟污染农田土壤可考虑采用植物和微生物联合修复措施，而对于低浓度污染土壤植物生长对细菌的促进效果不显著，可考虑采用其他修复措施。

Abstract:

In order to explore the impact of rare earth element (REE), lead and fluorine compound pollution on soil microbial communities, the microbial community functional diversity in soil was investigated with the Biolog-ECO system which collected at the farmland in high pollution (H zone) and low pollution area (L zone) of Maoniuping rare earth mining area, Sichuan Province. The results showed significant differences in the utilization ability of 31 carbon sources among different contaminated soil microorganisms ( P < 0.05). The utilization capacity and diversity index of non rhizosphere soil microorganisms in H zone were the lowest. There were only one carbon source with AWCDi ≥ 0.8 in non rhizosphere soil, and 27 species in rhizosphere soil, accounting for 87.1% of the total carbon source, which indicated plant growth significantly promoted the activity of rhizosphere bacteria. However, there were only 5 carbon sources with significant difference between rhizosphere and non rhizosphere in L zone ( P < 0.05). The RDA analysis showed that the rare earth complex pollution and C/N were all significantly negatively correlated with the average well color development( AWCD)，Shannon-wiener index and other diversity indexes in non-rhizosphere soil. The REE contributed 87.4% of the interpretation rate of microbial community characteristics, which was the main driver of microbial community change. While only 48% of the characteristic values were explained by the four environmental factors under the rhizosphere conditions, indicating that the participation of plants changed the impact of pollutants on microbial communities. It can be concluded that the combined phytoremediation and microbial remediation can be used to alleviate soil pollution in high concentrations of rare earth, lead and fluorine compound pollution. However, for low concentrations pollution, other remediation measures should be considered.

备注/Memo

备注/Memo:

收稿日期 Received: 2020-08-10 接受日期 Accepted: 2020-09-30
国家自然科学基金项目（41967033）、国家环境保护水土污染协同控制与联合修复重点实验室开放基金（GHBK-002）和四川省科技厅项目（19YYJC2826）资助
**通讯作者 Corresponding author (E-mail: liuguo@cdut.edu.cn)
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更新日期/Last Update: 2020-10-05