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[1]许小琼 张春渝 陈晓慧 张梓浩 林玉玲 陈振光 赖钟雄**.香蕉SUT基因家族的全基因组鉴定及其在不同低温胁迫下的表达[J].应用与环境生物学报,2021,27(04):1-19.[doi:10.19675/j.cnki.1006-687x.2020.07024]
 XU Xiaoqiong,ZHANG Chunyu,CHEN Xiaohui,et al.Genome-wide identification of SUT gene family and its expression analysis under different low temperature stress in Musa nana Lour.[J].Chinese Journal of Applied & Environmental Biology,2021,27(04):1-19.[doi:10.19675/j.cnki.1006-687x.2020.07024]
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香蕉SUT基因家族的全基因组鉴定及其在不同低温胁迫下的表达()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
27卷
期数:
2021年04期
页码:
1-19
栏目:
研究论文
出版日期:
2021-08-25

文章信息/Info

Title:
Genome-wide identification of SUT gene family and its expression analysis under different low temperature stress in Musa nana Lour.
作者:
许小琼 张春渝 陈晓慧 张梓浩 林玉玲 陈振光 赖钟雄**
福建农林大学园艺植物生物工程研究所 福州 350002
Author(s):
XU Xiaoqiong ZHANG Chunyu CHEN Xiaohui ZHANG Zihao LIN Yuling CHEN Zhenguang & LAI Zhongxiong**
Institute of Horticultural Plant Bioengineering, Fujian Agriculture and Forestry University, Fuzhou 35002, China
关键词:
香蕉蔗糖转运蛋白基因家族全基因组鉴定
Keywords:
Musa nana Lour. SUT gene family genome-wide identification
DOI:
10.19675/j.cnki.1006-687x.2020.07024
摘要:
为探究香蕉蔗糖转运蛋白(sucrose/H+cotransporter 或 sucrose transporters ,SUCs或SUTs)基因家族成员的分子特性及其在低温胁迫下的表达模式,采用生物信息学方法鉴定了香蕉蔗糖转运蛋白基因家族成员,并进行蛋白结构域及特性、基因及蛋白序列组分、分子进化树、基因启动子顺式作用元件以及在不同低温处理下表达模式的分析。结果显示:香蕉A(Musa acuminata)基因组与香蕉B(Musa balbisiana)基因组中各存在6条SUT基因,分别命名为MaSUT1-6、MbSUT1-6,且均分为3大类:SUT2、SUT3、SUT4;根据基因序列组分分析可知,MaSUT的GC含量低于MbSUT的GC含量,且二者均低于水稻SUT基因的GC含量;蛋白互作显示,MaSUT、MbSUT成员可与多种蛋白存在互作关系;MaSUT家族成员启动子包含激素、光、非生物胁迫响应相关元件,且该家族成员均包含光响应元件;MaSUT、MbSUT基因成员在不同低温胁迫处理下存在表达显著下调(4℃)、表达显著上调(28℃、13℃、4℃)、以及在各温度处理下均不表达的5种表达模式,而MaSUT1-2、MaSUT4、MbSUT1、MbSUT3-4在低温(13、4、0 ℃)处理下表达量均高于对照(28 ℃),MaSUT3和MbSUT2在低温处理下表达量则相对下降。本研究表明香蕉A、B基因组的SUT家族成员功能具有差异性,并且部分成员可能在香蕉响应低温胁迫的过程中发挥重要作用。
Abstract:
To explore the molecular characteristics of sucrose transporter (sucrose/H +cotransporter or sucrose transporters, SUCs or SUTs) gene family members in banana and their expression patterns under low temperature stress. In this study, bioinformatics methods were used to identify members of the banana sucrose transporter gene family, and analysis of the protein domains and characteristics, gene and protein sequence components, molecular evolutionary tree, gene promoter cis-acting elements, and expression patterns under different low temperature treatments .The results showed that there were 6 SUT genes in the banana A ( Musa acuminata) genome and the banana B (Musa balbisiana) genome, named as MaSUT1-6 and MbSUT1-6 respectively, and they were divided into 3 categories: SUT2, SUT3, SUT4; according to the analysis of gene sequence components, it coul d be seen that the GC content of MaSUT was lower than that of MbSUT, both MaSUT and MbSUT were lower than the GC content of rice SUT genes; protein interaction prediction showed that MaSUT and MbSUT members coul d interact with a variety of proteins; MaSUT family member promoters contained hormone, light, and abiotic stress response related elements, and the family members all contained light response elements; MaSUT and MbSUT gene members had the five expression patterns as follow, a significant down-regulation (4°C), a significant up-regulation (28°C, 13 ℃, 4℃) and not expression under each temperature treatment, the expression levels of MaSUT1-2, MaSUT4, MbSUT1, MbSUT3-4 were higher than those under low temperature (13, 4, 0 ℃) treatment. In contrast (28 ℃), the expression levels of MaSUT3 and MbSUT2 were relatively decreased under low temperature treatment. This study showed that the functions of SUT family members in banana A and B genomes were different, and some members might play an important role in banana response to low temperature stress.

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备注/Memo

备注/Memo:
收稿日期 Received: 2020-08-02 接受日期 Accepted: 2020-09-30
国家自然科学基金项目(31572088)、福建省高原学科建设经费(102/71201801101)和福建农林大学科技创新专项基金(KFA19037A,CXZX2017314)资助
**通信作者 Corresponding author(E-mail: Laizx01@163.com)
引用本文请注明出处本刊;发表刊期和页码将以正式出版时的安排为准,但DOI确定不变。
更新日期/Last Update: 2020-10-05