|本期目录/Table of Contents|

[1]彭婕 乔晋楠 李文馨 张义阳 张山 乔代蓉 曹毅**.全基因组挖掘与分析类芽孢杆菌SSG-1的甘露聚糖水解酶[J].应用与环境生物学报,2021,27(04):1-11.[doi:10.19675/j.cnki.1006-687x.2020.07066]
 PENG Jie,QIAO Jinnan,LI Wenxin,et al.Genome-wide mining and analysis of mannan hydrolases of Paenibacillus sp. SSG-1[J].Chinese Journal of Applied & Environmental Biology,2021,27(04):1-11.[doi:10.19675/j.cnki.1006-687x.2020.07066]
点击复制

全基因组挖掘与分析类芽孢杆菌SSG-1的甘露聚糖水解酶()
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
27卷
期数:
2021年04期
页码:
1-11
栏目:
研究论文
出版日期:
2021-08-25

文章信息/Info

Title:
Genome-wide mining and analysis of mannan hydrolases of Paenibacillus sp. SSG-1
作者:
彭婕1 乔晋楠2 李文馨1 张义阳1 张山1 乔代蓉1 曹毅1**
1四川大学生命科学学院微生物与代谢工程四川省重点实验室 成都 610065
2成都生物制品研究所有限责任公司 成都 500643
3深圳中科欣扬生物科技有限公司 深圳 518118
Author(s):
PENG Jie1 QIAO Jinnan2 LI Wenxin1 ZHANG Yiyang1 ZHANG Shan1 QIAO Dairong1 & CAO Yi1**
 1 Sichuan Key Laboratory of Microbiology and Metabolic Engineering, College of Life Sciences, Sichuan University, Chengdu, 610065, China
2 Chengdu Biological Products Research Institute Co. LTD, Chengdu, 500643, China
3 Shenzhen Zhongke Xinyang Biological Technology Co., LTD. Shenzhen, 518118, China
关键词:
 关键词:类芽孢杆菌甘露聚糖酶基因组挖掘生物信息分析
Keywords:
Paenibacillus mannanase genome mining bioinformatics analysis
DOI:
10.19675/j.cnki.1006-687x.2020.07066
摘要:
甘露聚糖(Mannan)是半纤维素中含量仅次于木聚糖的难水解多糖,发掘将甘露聚糖水解为甘露寡糖的甘露聚糖降解酶,对实现其在生物能源、制药、食品、饲料、制浆造纸和纺织等领域的应用,具有极其重要的意义。类芽孢杆菌作为甘露聚糖水解酶的新酶源,鲜有产甘露聚糖酶的报道。利用从土壤中分离获得的产琼胶酶类芽孢杆菌SSG-1的全基因组数据,采用生物信息技术,从全基因组角度挖掘其甘露聚糖水解酶。共挖掘获得6条甘露聚糖水解酶,经Pfam、CDD和分子进化分析,WP_089548806.1为GH26家族的内切β-1,4-甘露聚糖酶,WP_089549173.1和WP_089548729.1为GH76家族的α-1,6-甘露聚糖酶,WP_089547966.1、WP_089548481.1和WP_009225474.1为GH2家族的β-甘露糖苷酶。其中,WP_089548806.1和WP_089548729.1为分泌蛋白,多序列比对和结构分析表明WP_089548806.1具有内切β-1,4-甘露聚糖酶保守的底物结合和活性位点,具有β-甘露聚糖酶典型的(β/α)8-TIM桶状结构。本研究成功从类芽孢杆菌SSG-1中挖掘出6个新型甘露聚糖水解酶,可为挖掘新型甘露聚糖水解酶资源以及揭示类芽孢杆菌SSG-1甘露聚糖酶的表达调控机制奠定重要基础。(图4 表7 参19)
Abstract:
After xylan, mannan is the second most abundant hemicellulosic polysaccharide, which is difficult to hydrolyze. The discovery of mannan-degrading enzymes that hydrolyze mannan into mannose oligosaccharides is extremely important for the applications of mannan-degrading enzymes in the fields of bioenergy, pharmaceuticals, food, feed, pulp and paper and textile. As a potential source of mannan-degrading enzymes, Paenibacillus is rarely reported to produce mannanases. In this paper, the whole genome data analysis of the agarase-producing Paenibacillus sp. SSG-1 isolated from the soil and bioinformatics technology to mine its mannan hydrolase from the perspective of the whole genome. A total of 6 mannan-degrading enzymes were discovered by genome mining. The Pfam, CDD and molecular evolution analysis showed that WP_089548806.1 was mannan endo-1,4-beta-mannosidase belonging to GH26 family, while WP_089549173.1 and WP_089548729.1 were α-1,6-D-mannanases belonging to GH76 family, and WP_089547966.1, WP_089548481.1 and WP_009225474.1 were β-mannanases belonging to GH2 family. Among them, WP_089548806.1 and WP_089548729.1 were secreted proteins. Multiple sequence alignment and structural analysis showed that WP_089548806.1 had the conserved substrate binding and active site of mannan endo-1,4-beta-mannosidase, and exhibited the typical mannanase structure of (β/α)8-TIM barrel.fold. In this study, 6 new mannan hydrolases were successfully excavated from Paenibacillus sp. SSG-1. This work has laid a solid foundation for the discovery of the novel mannan-degrading enzymes and for the future work to explore the potential mechanism on the regulation of mannanase expression in Paenibacillus sp. SSG-1.

备注/Memo

备注/Memo:
收稿日期 Received: 2020-07-30 接受日期 Accepted: 2020-09-30
四川省科技厅项目(2018NZ0007,2020YJ0304,2020YJ0305,2020YFH0076)、国家微生物资源平台项目(NIMR-2019-8-1)和四川省微生物资源平台项目(2019JDPT0012)资助
**通讯作者 Corresponding author (E-mail: cyi@scu.edu.cn)
引用本文请注明出处本刊;发表刊期和页码将以正式出版时的安排为准,但DOI确定不变。
更新日期/Last Update: 2020-10-05