|本期目录/Table of Contents|

[1]韩婕 陈晓慧 申序 李晓斐 林玉玲 吴鹏飞** 赖钟雄**.龙眼SAP-PPD-KIX-TPL信号途径的基因家族的全基因组鉴定与表达模式[J].应用与环境生物学报,2021,27(04):1-17.[doi:10.19675/j.cnki.1006-687x.2020.07024]
　HAN Jie,CHEN Xiaohui,et al.Genome-wide identification and expression pattern of gene family of SAP-PPD-KIX-TPL signal pathway in Dimocarpus longan Lour. *[J].Chinese Journal of Applied & Environmental Biology,2021,27(04):1-17.[doi:10.19675/j.cnki.1006-687x.2020.07024]
点击复制

龙眼SAP-PPD-KIX-TPL信号途径的基因家族的全基因组鉴定与表达模式()
分享到：

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
27卷
期数:
2021年04期
页码:
1-17
栏目:
研究论文
出版日期:
2021-08-25

文章信息/Info

Title:
Genome-wide identification and expression pattern of gene family of SAP-PPD-KIX-TPL signal pathway in Dimocarpus longan Lour. *
作者:
韩婕1; 2 陈晓慧1 申序1 李晓斐1 林玉玲1 吴鹏飞2** 赖钟雄1**
1福建农林大学园艺植物生物工程研究所 福州 350002
2福建农林大学林学院 福州 350002
Author(s):
HAN Jie1;  2;  CHEN Xiaohui1;  SHEN Xu1;  LI Xiaofei1;  LIN Yuling1;  WU Pengfei1;  2** & LAI Zhongxiong1**
1 Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
2 Forestry College, Fujian Agriculture and Forestry University, Fuzhou 350002, China
关键词:
龙眼; SAP-PPD-KIX-TPL信号途径; 成员鉴定; 生物信息学分析; 实时荧光定量PCR
Keywords:
Dimocarpus longan Lour. ;  SAP-PPD-KIX-TPL signal pathway;  Members identification;  Bioinformatics analysis;  qRT-PCR
DOI:
10.19675/j.cnki.1006-687x.2020.07024
摘要:
STERILE APETALA（SAP）可调控PPD-KIX-TPL转录抑制复合体的稳定性，从而调节器官大小。基于龙眼基因组数据库，对龙眼SAP-PPD-KIX-TPL信号途径的基因家族进行全基因组鉴定，分析龙眼SAP、PEAPOD（PPD）和TOPLESS(TPL)家族成员的基本理化性质、基因结构及蛋白结构域、启动子顺式作用元件、蛋白互作网络、构建系统进化树；分析龙眼体胚发生过程及不同组织部位中的基因表达水平，并采用qRT-PCR检测体胚发生早期及不同激素处理下的表达模式。结果表明,DlSAP包含1个成员，具有WD40结构域，只有1个内含子。DlPPD包含7个成员，具有tify和CCT_2结构域，含有2-7个内含子，最保守基序为motif1。DlTPL包含6个成员，具有LisH、CTLH和WD40结构域，含有23-29个内含子，除motif7外，motif1-10中的剩余基序均保守。龙眼kinase-inducible domain interacting（KIX）家族包含8个成员，保守结构域为KIX。顺式作用元件预测表明，DlSAP、DlPPD和DlTPL启动子均包含大量光响应、激素应答和非生物胁迫响应元件。蛋白互作网络预测显示，SAP和PPD之间的互作关系较强，与TPL相关联的蛋白较多。qRT-PCR 结果表明，DlSAP、DlTIFY4B和DlTPR2-1在球形胚阶段表达量最高；DlSAP、DlTIFY10B-1和DlTPR2-1在不同激素处理中均出现多种表达模式。总之，龙眼SAP-PPD-KIX-TPL信号途径的基因可能参与响应多种激素和非生物胁迫应答、也可能参与胚胎发育，具有多种生物学功能。（图9 表2 参33）
Abstract:
STERILE APETALA (SAP) can regulate the stability of PPD-KIX-TPL transcriptional repressor complex, thus regulating organ size.Based on longan genomic database, this study identified the gene family of SAP-PPD-KIX-TPL signaling pathway in longan, analyzed the basic physical and chemical properties, gene structure and protein domain, cis-acting element of promoters, protein interaction network of SAP, PEAPOD(PPD) and TOPLESS(TPL) family in longan, and constructed phylogenetic tree; analyzed the gene expression in the process of longan embryogenesis and different tissues, and qRT-PCR was used to detect the expression patterns in early somatic embryogenesis and different hormone treatments. The results show that DlSAP contains 1 member, has WD40 domain and only 1 intron. DlPPD contains 7 members, has tify and CCT_2 domain, contains 2-7 introns, and the most conservative motif is motif1. DlTPL has 6 members, has LisH、CTLH and WD40domain, and 23-29 introns. Except for motif7, the remaining motif in motif1-10 are conserved. Kinase-inducible domain interacting (KIX) family of longan contains 8 members with conserved domain KIX. Cis-acting element predicted that DlSAP、DlPPD and DlTPL promoters contained a large number of light response, hormone response and abiotic stress response components. Protein interaction network prediction shows that the interaction between SAP and PPD is strong, and there are more proteins associated with TPL. These results of qRT-PCR showed that the expression of DlSAP, DlTIFY4B and DlTPR2-1 was highest in spherical embryo. DlSAP, DlTIFY10B-1 and DlTPR2-1 showed multiple expression patterns in different hormone treatments. In conclusion, the SAP-PPD-KIX-TPL signal pathway genes of longan may participate in and response to various hormones and abiotic stresses, and may also be involved in embryonic development with a variety of biological functions.

相似文献/References:

[1]邱栋梁,刘星辉,王湘平.模拟酸雨对龙眼叶绿体的伤害效应[J].应用与环境生物学报,2002,8(02):154.
　QIU Dongliang,et al..Injury effects of simulated acid rain on chloroplasts of longan leaves[J].Chinese Journal of Applied & Environmental Biology,2002,8(04):154.
[2]邱栋梁,刘星辉,郭素枝.模拟酸雨对龙眼幼果纤维素酶活性和内源激素含量的影响[J].应用与环境生物学报,2004,10(01):35.
　QIU Dongliang,et al..Effects of simulated acid rain on cellulase activity and contents of endogenous hormone in young fruit of longan[J].Chinese Journal of Applied & Environmental Biology,2004,10(04):35.
[3]林玉玲,赖钟雄.龙眼胚性愈伤组织Cu/Zn-SOD分子伴侣基因CCS的克隆及其在体胚发生过程中的表达分析[J].应用与环境生物学报,2012,18(03):351.[doi:10.3724/SP.J.1145.2012.00351]
　LIN Yuling,LAI Zhongxiong.Cloning of Copper Chaperone for Superoxide Dismutase Gene CCS from Embryogenic Callus of Dimocarpus longan Lour. and Its Expression Analysis During Somatic Embryogenesis[J].Chinese Journal of Applied & Environmental Biology,2012,18(04):351.[doi:10.3724/SP.J.1145.2012.00351]
[4]赖瑞联,林玉玲,赖钟雄.龙眼生长素受体基因TIR1的克隆及其与miR393互作关系[J].应用与环境生物学报,2016,22(01):95.[doi:10.3724/SP.J.1145.2015.05051]
　LAI Ruilian,LIN Yuling & LAI Zhongxiong**.Cloning of auxin receptor gene TIR1 and its interaction with miR393 in Dimocarpus longan Lour.[J].Chinese Journal of Applied & Environmental Biology,2016,22(04):95.[doi:10.3724/SP.J.1145.2015.05051]
[5]陈旭,曾友竞,王嘉毅,等.龙眼miR159家族成员进化特性及时空表达[J].应用与环境生物学报,2017,23(04):602.[doi:10.3724/SP.J.1145.2017.03011]
　CHEN Xu,ZENG Youjing,WANG Jiayi,et al.Effect of main grain components on the starch swelling power of Tibetan hull-less barley (Hordeum vulgare var. nudum)[J].Chinese Journal of Applied & Environmental Biology,2017,23(04):602.[doi:10.3724/SP.J.1145.2017.03011]
[6]王云,彭丽云,孙雪丽,等.龙眼Hsf基因家族全基因组鉴定及体胚发生过程中的表达分析[J].应用与环境生物学报,2019,25(02):420.[doi:10.19675/j.cnki.1006-687x.2018.06004]
　WANG Yun,PENG Liyun,SUN Xueli,et al.Genome-wide identification of longan Hsf family members and their functional analysis during somatic embryogenesis in longan[J].Chinese Journal of Applied & Environmental Biology,2019,25(04):420.[doi:10.19675/j.cnki.1006-687x.2018.06004]
[7]苏立遥,黄倏祺,蒋梦琦,等.龙眼miR403及其候选靶标对外源激素的响应模式以及在龙眼体胚中的表达模式[J].应用与环境生物学报,2019,25(04):977.[doi:10.19675/j.cnki.1006-687x.2019.03058]
　SU Liyao,HUANG Shuqi,JIANG Mengqi,et al.The response patterns of miR403 and its candidate targets to exogenous hormones and their expression profiles in the longan somatic embryo[J].Chinese Journal of Applied & Environmental Biology,2019,25(04):977.[doi:10.19675/j.cnki.1006-687x.2019.03058]
[8]李汉生,孙刚,陈晓慧,等.龙眼BRI1基因家族的全基因组鉴定及光照响应表达[J].应用与环境生物学报,2020,26(01):125.[doi:10.19675/j.cnki.1006-687x.2019.07053]
　LI Hansheng,SUN Gang,CHEN Xiaohui,et al.Genome-wide identification and response light expression analysis of the BRI1 family in Dimocarpus longan Lour[J].Chinese Journal of Applied & Environmental Biology,2020,26(04):125.[doi:10.19675/j.cnki.1006-687x.2019.07053]
[9]刘蒲东,张舒婷,陈晓慧,等.龙眼GRF家族全基因组鉴定及表达模式[J].应用与环境生物学报,2020,26(02):236.[doi:10.19675/j.cnki.1006-687x.2019.06028]
　LIU Pudong,ZHANG Shuting,CHEN Xiaohui,et al.Genomic identification and expression patterns of the longan GRF family[J].Chinese Journal of Applied & Environmental Biology,2020,26(04):236.[doi:10.19675/j.cnki.1006-687x.2019.06028]
[10]廖斌,徐小萍,李珊珊,等.苯丙氨酸和茉莉酸甲酯对龙眼胚性悬浮细胞柯里拉京积累的影响[J].应用与环境生物学报,2020,26(02):287.[doi:10.19675/j.cnki.1006-687x.2019.06001]
　LIAO Bin,XU Xiaoping,LI Shanshan,et al.Effects of phenylalanine and methyl jasmonate on the growth and corilagin accumulation of embryogenic suspension cells in Dimocarpus longan Lour.[J].Chinese Journal of Applied & Environmental Biology,2020,26(04):287.[doi:10.19675/j.cnki.1006-687x.2019.06001]

备注/Memo

备注/Memo:
收稿日期 Received: 2020-07-13 接受日期 Accepted: 2020-09-30
国家自然科学基金项目（31572088，31672127）和福建省高原学科建设经费（102/71201801101）资助
**通讯作者 Corresponding author (E-mail: Laizx01@163.com; 278613589@qq.com)
引用本文请注明出处本刊；发表刊期和页码将以正式出版时的安排为准，但DOI确定不变。
更新日期/Last Update: 2020-10-05