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[1]刘嘉鹏# 谭秋月# 伍俊为 王斌 田娜 刘范 孙雪丽 程春振**.香蕉MaTIFY9基因的克隆及表达[J].应用与环境生物学报,2021,27(03):1-10.[doi:10.19675/j.cnki.1006-687x.2020.07006]
　LIU Jiapeng#,TAN Qiuyue#,WU Junwei,et al.Cloning and expression analysis of a MaTIFY9 gene in banana[J].Chinese Journal of Applied & Environmental Biology,2021,27(03):1-10.[doi:10.19675/j.cnki.1006-687x.2020.07006]
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香蕉MaTIFY9基因的克隆及表达()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
27卷
期数:
2021年03期
页码:
1-10
栏目:
出版日期:
2021-06-25

文章信息/Info

Title:
Cloning and expression analysis of a MaTIFY9 gene in banana
作者:
刘嘉鹏# 谭秋月# 伍俊为 王斌 田娜 刘范 孙雪丽 程春振**
福建农林大学园艺学院 福州 350002
Author(s):
LIU Jiapeng#;  TAN Qiuyue#;  WU Junwei;  WANG Bin;  TIAN Na;  LIU Fan;  SUN Xueli;  CHENG Chunzhen**
College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 35002, China
关键词:
香蕉; MaTIFY9; 基因克隆; 生物信息学分析; 表达模式
Keywords:
banana;  MaTIFY9;  gene cloning;  bioinformatics analysis;  expression pattern
DOI:
10.19675/j.cnki.1006-687x.2020.07006
摘要:
为探究一个受低温诱导的香蕉MaTIFY9基因的序列特征及在不同激素和逆境胁迫处理下的表达模式，分别使用PCR和反转录PCR（RT-PCR）技术克隆该基因的gDNA和cDNA序列，分析其核苷酸和编码蛋白序列特性，利用实时荧光定量PCR（qRT-PCR）技术研究其在不同器官和不同激素、逆境胁迫处理下的表达情况。结果显示：MaTIFY9编码了一个分子式为C841H1346N236O249S7，氨基酸大小为172 aa，分子量为18989.93，等电点为9.55的不稳定亲水性蛋白。MaTIFY9无信号肽和跨膜结构，具有TIFY和Jas（CCT-2）两个保守结构域，属于JAZ亚家族，与小果野蕉TIFY9亲缘关系最近。亚细胞定位预测结果显示MaTIFY9主要定位于细胞核。MaTIFY9启动子含有大量的光响应相关元件，此外还含有许多防御及应激、SA和ABA响应元件。qRT-PCR结果显示MaTIFY9在根中表达量最高，其次是假茎和球茎，叶最低；其表达受ABA、SA和枯萎病显著诱导，受高温抑制。与其他植物TIFY类似，MaTIFY表达受多种激素和逆境调控，可能在香蕉响应非生物和生物逆境胁迫过程中发挥重要作用。（图6 表3 参32）
Abstract:
In order to investigate the sequence characteristics and the expression pattern under different phytohormone and abiotic stress treatments of a low temperature inducible banana MaTIFY9 gene , its gDNA and cDNA sequence was successfully amplified by PCR and reverse transcription PCR (RT-PCR), respectively. Series of bioinformatic analysis was performed to reveal the characteristics of its nucleotide and encoded protein sequences . Its expression patterns in different tissues and organs and under different treatments were analyzed using quantitative real time PCR ( qRT-PCR). MaTIFY9 encodes an unstable hydrophilic protein of 172 aa with molecular formula of C841H1346N236O249S7, molecular weight of 18989.93 Da, and an isoelectric point of 9.55. MaTIFY9 owns no signal peptide and transmembrane structure , but owns two conserved domains ( TIFY and Jas), and is thus estimated to be a member of JAZ subfamily. Phylogenic relationship analysis revealed that the MaTIFY9 protein shared the closest relationship with the Musa acuminata subsp. malaccensis TIFY 9. Protein subcellular localization prediction result showed that MaTIFY9 was mainly loca ted in the nucleus. The promoter of MaTIFY9 contains not only a large number of light responsive elements, but also a large number of defense and stress-, SA - and ABA -responsiveness elements. qRT-PCR results showed that MaTIFY9 expressed the highest in root, followed by pseudostem and corm, and the lowest in leaf. Its expression could be significantly induced by ABA, SA and Fusarium wilt treatments , but was inhibited by high temperature. Similar to the TIFYs from other plant species, MaTIFY9 is responsive to various phytohormone and stress treatments , and may play important roles in the banana responses to both abiotic and biotic stresses.

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备注/Memo

备注/Memo:

收稿日期 Received: 2020-07-06 接受日期 Accepted: 2020-09-04
国家重点研发计划课题（2019YFD1000901）、国家自然科学基金项目（31601713）和国家现代农业产业技术体系建设专项资金项目（CARS-32-11）资助 Supported by the National Key R&D Program of China (2019YFD1000901), the Natural Science Foundation of China (31601713) and the National Special Fund Project For the Construction of Modern Agricultural Technology System (CARS-32-11)
*并列第一作者 Co-first authors
**通讯作者 Corresponding author (E-mail: ld0532cheng@126.com)
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更新日期/Last Update: 2020-09-10