|本期目录/Table of Contents|

[1]刘嘉鹏# 谭秋月# 伍俊为 王斌 田娜 刘范 孙雪丽 程春振**.香蕉MaTIFY9基因的克隆及表达[J].应用与环境生物学报,2021,27(03):1-10.[doi:10.19675/j.cnki.1006-687x.2020.07006]
 LIU Jiapeng#,TAN Qiuyue#,WU Junwei,et al.Cloning and expression analysis of a MaTIFY9 gene in banana[J].Chinese Journal of Applied & Environmental Biology,2021,27(03):1-10.[doi:10.19675/j.cnki.1006-687x.2020.07006]
点击复制

香蕉MaTIFY9基因的克隆及表达()
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
27卷
期数:
2021年03期
页码:
1-10
栏目:
出版日期:
2021-06-25

文章信息/Info

Title:
Cloning and expression analysis of a MaTIFY9 gene in banana
作者:
刘嘉鹏# 谭秋月# 伍俊为 王斌 田娜 刘范 孙雪丽 程春振**
福建农林大学园艺学院 福州 350002
Author(s):
LIU Jiapeng# TAN Qiuyue# WU Junwei WANG Bin TIAN Na LIU Fan SUN Xueli CHENG Chunzhen**
College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 35002, China
关键词:
香蕉MaTIFY9基因克隆生物信息学分析表达模式
Keywords:
banana MaTIFY9 gene cloning bioinformatics analysis expression pattern
DOI:
10.19675/j.cnki.1006-687x.2020.07006
摘要:
为探究一个受低温诱导的香蕉MaTIFY9基因的序列特征及在不同激素和逆境胁迫处理下的表达模式,分别使用PCR和反转录PCR(RT-PCR)技术克隆该基因的gDNA和cDNA序列,分析其核苷酸和编码蛋白序列特性,利用实时荧光定量PCR(qRT-PCR)技术研究其在不同器官和不同激素、逆境胁迫处理下的表达情况。结果显示:MaTIFY9编码了一个分子式为C841H1346N236O249S7,氨基酸大小为172 aa,分子量为18989.93,等电点为9.55的不稳定亲水性蛋白。MaTIFY9无信号肽和跨膜结构,具有TIFY和Jas(CCT-2)两个保守结构域,属于JAZ亚家族,与小果野蕉TIFY9亲缘关系最近。亚细胞定位预测结果显示MaTIFY9主要定位于细胞核。MaTIFY9启动子含有大量的光响应相关元件,此外还含有许多防御及应激、SA和ABA响应元件。qRT-PCR结果显示MaTIFY9在根中表达量最高,其次是假茎和球茎,叶最低;其表达受ABA、SA和枯萎病显著诱导,受高温抑制。与其他植物TIFY类似,MaTIFY表达受多种激素和逆境调控,可能在香蕉响应非生物和生物逆境胁迫过程中发挥重要作用。(图6 表3 参32)
Abstract:
In order to investigate the sequence characteristics and the expression pattern under different phytohormone and abiotic stress treatments of a low temperature inducible banana MaTIFY9 gene , its gDNA and cDNA sequence was successfully amplified by PCR and reverse transcription PCR (RT-PCR), respectively. Series of bioinformatic analysis was performed to reveal the characteristics of its nucleotide and encoded protein sequences . Its expression patterns in different tissues and organs and under different treatments were analyzed using quantitative real time PCR ( qRT-PCR). MaTIFY9 encodes an unstable hydrophilic protein of 172 aa with molecular formula of C841H1346N236O249S7, molecular weight of 18989.93 Da, and an isoelectric point of 9.55. MaTIFY9 owns no signal peptide and transmembrane structure , but owns two conserved domains ( TIFY and Jas), and is thus estimated to be a member of JAZ subfamily. Phylogenic relationship analysis revealed that the MaTIFY9 protein shared the closest relationship with the Musa acuminata subsp. malaccensis TIFY 9. Protein subcellular localization prediction result showed that MaTIFY9 was mainly loca ted in the nucleus. The promoter of MaTIFY9 contains not only a large number of light responsive elements, but also a large number of defense and stress-, SA - and ABA -responsiveness elements. qRT-PCR results showed that MaTIFY9 expressed the highest in root, followed by pseudostem and corm, and the lowest in leaf. Its expression could be significantly induced by ABA, SA and Fusarium wilt treatments , but was inhibited by high temperature. Similar to the TIFYs from other plant species, MaTIFY9 is responsive to various phytohormone and stress treatments , and may play important roles in the banana responses to both abiotic and biotic stresses.

相似文献/References:

[1]袁先福,孙玉菡,朱成之,等.轮作联用生物有机肥促进香蕉生长[J].应用与环境生物学报,2018,24(01):60.[doi:10.19675/j.cnki.1006-687x.2017.03022]
 YUAN Xianfu,SUN Yuhan,ZHU Chengzhi,et al.Rotation combined with bio-organic fertilizer application to promote banana growth[J].Chinese Journal of Applied & Environmental Biology,2018,24(03):60.[doi:10.19675/j.cnki.1006-687x.2017.03022]
[2]黄玉吉,赖钟雄.香蕉几丁质酶基因ChiI2超表达载体和干涉表达载体的构建[J].应用与环境生物学报,2019,25(03):672.[doi:10.19675/j.cnki.1006-687x.201809019]
 HUANG Yuji LAI Zhongxiong**.Over-expression and RNAi expression vector construction for the ChiI2 gene of banana[J].Chinese Journal of Applied & Environmental Biology,2019,25(03):672.[doi:10.19675/j.cnki.1006-687x.201809019]
[3]孙雪丽,刘范,田娜,等.香蕉MaMPK1基因的克隆与表达模式[J].应用与环境生物学报,2019,25(04):985.[doi:10.19675/j.cnki.1006-687x.2018.10027]
 SUN Xueli,LIU Fan,TIAN Na,et al.Cloning and expression pattern analysis of the banana MaMPK1 gene[J].Chinese Journal of Applied & Environmental Biology,2019,25(03):985.[doi:10.19675/j.cnki.1006-687x.2018.10027]
[4]田娜,刘范,孙雪丽,等.水培炼苗对香蕉组培移栽苗生长及光合特性的影响[J].应用与环境生物学报,2020,26(03):582.[doi:10.19675/j.cnki.1006-687x.2019.06029]
 TIAN Na,LIU Fan,SUN Xueli,et al.Effects of hydroponic culture hardening on the growth and photosynthetic characteristics of banana seedlings[J].Chinese Journal of Applied & Environmental Biology,2020,26(03):582.[doi:10.19675/j.cnki.1006-687x.2019.06029]
[5]项蕾蕾,李丹,孙雪丽,等.LED补光对香蕉组培苗增殖和生理生化指标的影响[J].应用与环境生物学报,2020,26(03):590.[doi:10.19675/j.cnki.1006-687x.2019.08038]
 XIANG Leilei,LI Dan,SUN Xueli,et al.Effects of LED supplementation on proliferation, and physiological and biochemical indices of tissue-cultured banana plantlets[J].Chinese Journal of Applied & Environmental Biology,2020,26(03):590.[doi:10.19675/j.cnki.1006-687x.2019.08038]
[6]刘嘉鹏# 刘萍元# 孙雪丽 刘范 田娜 伍俊为 程春振**.香蕉含VQ基序蛋白基因MaVQ1的克隆与表达[J].应用与环境生物学报,2021,27(02):1.[doi:10.19675/j.cnki.1006-687x.2020.03026]
 LIU Jiapeng#,LIU Pingyuan#,SUN Xueli,et al.Cloning and expression analysis of a VQ motif containing protein gene in banana[J].Chinese Journal of Applied & Environmental Biology,2021,27(03):1.[doi:10.19675/j.cnki.1006-687x.2020.03026]
[7]许小琼 张春渝 陈晓慧 张梓浩 林玉玲 陈振光 赖钟雄**.香蕉SUT基因家族的全基因组鉴定及其在不同低温胁迫下的表达[J].应用与环境生物学报,2021,27(04):1.[doi:10.19675/j.cnki.1006-687x.2020.07024]
 XU Xiaoqiong,ZHANG Chunyu,CHEN Xiaohui,et al.Genome-wide identification of SUT gene family and its expression analysis under different low temperature stress in Musa nana Lour.[J].Chinese Journal of Applied & Environmental Biology,2021,27(03):1.[doi:10.19675/j.cnki.1006-687x.2020.07024]

备注/Memo

备注/Memo:

收稿日期 Received: 2020-07-06 接受日期 Accepted: 2020-09-04
国家重点研发计划课题(2019YFD1000901)、国家自然科学基金项目(31601713)和国家现代农业产业技术体系建设专项资金项目(CARS-32-11)资助 Supported by the National Key R&D Program of China (2019YFD1000901), the Natural Science Foundation of China (31601713) and the National Special Fund Project For the Construction of Modern Agricultural Technology System (CARS-32-11)
*并列第一作者 Co-first authors
**通讯作者 Corresponding author (E-mail: ld0532cheng@126.com)
点击摘要页题目后的“PDF”可下载阅读全文;本文为已录用的作者修定稿,尚未经编辑全面修改。
引用本文请注明出处本刊;发表刊期和页码将以正式出版时的安排为准,但DOI确定不变。


 

更新日期/Last Update: 2020-09-10